Indian Drugs 44 (3), 205-208 (2007). HPTLC of frusemide (= furosemide) and spironolactone on silica gel with toluene - acetonitrile - glacial acetic acid 70:30:2, with chamber saturation for 15 min at room temperature. Development over 8 cm, followed by air drying. Quantitative determination by densitometry at 254 nm. Linearity was between 8 - 32 ng/µL and 20 - 80 ng/µL for frusemide and spironolactone respectively. The method was validated for accuracy and precision. The limit of detection and quantification for frusemide was 3 ng/µL and 8 ng/µL respectively, and for spironolactone 2 ng/µL and 6 ng/µL, respectively. Recovery by standard addition was 99.4-101% for both compounds.

Chromatographia, 66 (1-2), 95-102 (2007). Description of two sensitive and reproducible methods for the quantitative determination of dasatinib in the presence of its degradation products. HPTLC on silica gel with toluene - chloroform 7:3, followed by densitometric measurement at 280 nm. Validation of both separation methods according to ICH guidelines, no interference from the tablet excipients was found. Discussion of application of the methods as the stability indication because the proposed analytical methods could effectively separate the drug from its degradation products, which was subjected to acid–alkali hydrolysis, oxidation, dry heat, wet heat and photo-degradation.

Thieme Medical Publishers Inc., New York (2006). This book presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the techniques and common applications of HPTLC in herbal analysis. Chapters covered are theoretical concepts (stationary phase, mobile phase, TLC results, densitometry), practical aspects of modern TLC (sample preparation, selecting the stationary phase, sample application, chromatogram development, derivatization, documentation, reporting and record keeping, TLC software, standardization), typical applications in herbal analysis, method development, and validation of qualitative and quantitative HPTLC methods.

J. Planar Chromatogr. 20, 411-417 (2007). HPTLC of sucralose in dietetic products on silica gel impregnated with 0.1 M dipotassium hydrogen phosphate solution, and on amino phase with acetonitrile - water 17:3. Also a mixture of sucralose, sucrose, glucose, fructose was separated on amino phases with acetonitrile - water 3:1. Detection by dipping in 2-naphthol sulfuric acid reagent and aniline diphenylamine ortho-phosphoric acid reagent, followed by heating at 120 °C. Post-chromatographic derivatization on aluminium-backed amino plates was performed by heating the plate 190 °C for 20 min. Evaluation under UV light at 366 nm. For fluorescence enhancement the amino plate was dipped into a 1:2 solution of paraffin in n-hexane. Densitometric quantification by fluorescence measurement at 366 nm and by absorbance measurement at 500 and 405 nm.

J. Planar Chromatogr. 20, 49-51 (2007). HPTLC of mimosine (alpha-amino-3-hydroxy-4-oxo-1(4H)-pyridinepropanoic acid) on RP-18 with ethyl acetate - glacial acetic acid - water 60:10:17. Quantitation by densitometric scanning at 282 nm in absorbance mode. Limit of detection was 20 mg/g in the whole plant powder.

Biomed. Chromatogr. 20 (2), 151-153 (2005). TLC of the four principal triterpenoid components of Centella asiatica on silica gel plates with the combination of ethyl acetate and methanol. Detection by spraying with anisaldehyde solution, followed by heating at 100 °C for 5 min. Evaluation under white light. The developed method is a modification of the method described in the European Pharmacopoeia (5th edn). Confirmation of the separated compounds by MALDI-TOF mass spectrometry.

J. AOAC Int. 90, 1203-1209 (2007). HPTLC of terpenelactones (total bilobalide, gingkolide A, and gingkolide B) on prewashed and sodium acetate preimpregnated silica gel with toluene - ethyl acetate - acetone - methanol 50:25:25:3 or ethyl acetate - hexane 9:1; also HPTLC of flavonol glycosides (quercetin, kaempferol, isorhamnetin as standards) on prewashed and preimpregnated silica gel with chloroform - acetone - formic acid - acetic acid 50:11:6:6. Plates were developed in solvent equilibrated, vapor saturated twin-trough chambers at 30°C. Densitometry in absorbance mode at 370 nm (for aglycones) and at 290 nm following a 1 s immersion in acetic anhydride and heating at different temperatures for varying lengths of time (for terpenelactones). Good relationship (95%) was determined between HPTLC and HPLC for determination of total flavonol glycosides. The HPTLC flavonol aglycone method also performed well in terms of accuracy and consecutive plate repeatability.

J. Planar Chromatogr. 20, 463-475 (2007). TLC of chlorpromazine hydrochloride, trifluoperazine dihydrochloride, promazine hydrochloride, and doxepin hydrochloride on silica gel with 1-butanol - aqueous ammonia solution 5:1 or cyclohexane - acetone - diethylamine 8:1:1 after saturation with mobile phase vapor. Detection by inspection under UV light at 254 nm. Scanning densitometry was performed at 254 nm and at the wavelengths of maximum absorbance of the substances.