Phytochemistry. 155, 1-11 (2018). Review of recent developments in plant sources of propolis and innovative phytochemical approaches to propolis analysis, including TLC application for the assessment of its authenticity and HPTLC fingerprinting and HPTLC-DART-MS for metabolomic approaches in determining geographical origin.

J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

J. Chromatogr. A 1572, 152-161 (2018). Description of a novel application of HPTLC for separation and quantification of eight food colors (amaranth, allura red, erythrosine, fast green, indigo carmine, quinoline yellow, sunset yellow and tartrazine) and four intense sweeteners (acesulfame, aspartame, saccharin and neohesperidin) on silica gel with acetonitrile – water - ethyl acetate - 10 % aqueous ammonia 9:1:1:1. Quantitative determination by densitometry at 210, 295, 450 and 550 nm. The LOD and LOQ ranged from 0.2 to 10 ng/zone and from 1 to 30 ng/zone, respectively. The correlation coefficient (r2) values for the calibration curves were ≥ 0.9973 for all the additives. The recovery of different additives obtained from food products, beverages and pharmaceuticals ranged from 96.6 to 106.7 %.

Proc. Euro Food Pack, Intern. Conference on food packing, Vienna, 275-279 (1984). Two-dimensional TLC of 5-cholestene 3 beta,7 alpha-diol; 3,5-cholestadiene-7-one; cholestan-3 beta, 5 alpha, 6 beta-triol on silica with 1) chloroform - acetone 9:1, 2) chloroform - ether 1:1. Detection by UV. TLC study oxidation of cholesterol in heated tallow, photooxidation of cholesterol in butter.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 65 (1984). OPLC (OPTLC) of cannabinoids on silica impregnated with DMF, with a) cyclohexane, b) toluene- dioxan 60:1. Detection by UV and with 0.2 % Fast Blue B reagent. Densitometry by absorbance.

(Thin-layer densitometric determination of clotiazepam in tablets and blood plasma.) Arzneim. Forsch. 35, 547-551 (1984). TLC of clotiazepam on silica with toluene - methanol 9. 1. Scanning by fluorescence. Detection limit 2.5 ng/ml, inaccuracy between 25 and 300 ng/nl ± 5 %; imprecision at 100 ng/ml ± 5 %.

Proc. of Euro Food Chem. III, 2, 216-223, March 1985, Antwerp, Belgium. Consecutive extraction with sodium carbonate and ether; TLC on silica with benzene; in-situ derivatization with p-bromophenacylbromide; densitometric scanning by absorbance; detection limit 50 ng; good precision.

Application to real samples. J. High Resol. Chromatogr. 8, 135-139 (1985). Separation of inorganic and organic mercury dithizonates on silica (after conditioning with 5 M NH3) with hexane - acetone 85:15. Quantification by densitometry at 470 nm.