J. Liq. Chromatogr. Relat. Technol. 38, 1160-1163 (2015). HPTLC of glucose and maltose in Biomphalaria glabrata after coexposure with Echinostoma caproni and Schistosoma mansoni on silica gel with 1-butanol - glacial acetic acid - diethyl ether - deionized water 27:18:5:3. Detection by spraying with 5 g 1-naphthol and 20 mL concentrated sulfuric acid in 160 mL ethanol and 13 mL deionized water, followed by heating at 110 °C for 10 min. Quantitative determination by absorbance measurement at 515 nm. The hRF values of maltose and glucose were 40 and 50, respectively.

J. Planar Chromatogr. 28, 229-233 (2015). HPTLC of paroxetine in human serum on silica gel with toluene - acetone - ethanol - ammonia 25 % in water 9:5:2:1. Quantitative determination by absorbance measurement at 294 nm. Linearity was between 0.01 and 0.14 ng/µL. The intra-day precisions were between 0.9 % and 2.2 % (n=5) and the inter-day precisions were between 1.0 % and 3.9 % (n=9). The LOD and LOQ was 0.3 and 0.6 ng/zone, respectively. Recoveries were between 91.5 % and 96.5 %.

Food Chem. 197, 285-290 (2016). HPTLC of caffeic acid (1), gallic acid (2), resveratrol (3) and rutin (4) in wine on silica gel in a two step elution with dichlormethane - methanol - formic acid 7:20:7 and with a water-in-oil microemulsion consisting of sodium dodecyl sulphate - butanol - water - heptane 8:25:8:160. Detection by spraying with 1 % 2-aminoethyl diphenylborinate solution followed by heating at 50 °C for 30 min. Quantitative determination by absorbance measurement at 366 nm. The hRF values for (1) to (4) were 67, 43, 77 and 21, respectively. Linearity was in the range of 0.06-0.02 μg/zone for (1), 0.2-1.2 μg/zone for (2), 0.5-5.0 μg/zone for (3) and 0.03-3.0 μg/zone for (4). LOD was 20 ng/zone for (1), 68 ng/zone for (2), 150 ng/zone for (3) and 9 ng/zone for (4). The intermediate precision was below 5 % (n=3). Recovery was in the range of 76-120 % for (1) to (4).

Pharm. Res. 33, 328-336 (2016). HPTLC of a derivative of a novel D-peptide with improved binding to Aβ oligomers (3H-radioactively labelled RD2) on silica gel with 2-butanol - pyridine - ammonia (28 %) - water 39:34:10:26. 3H detection by placing the plates on phosphor imaging plates for autoradiography. The method was useful to determine the stability of 3H-labelled RD2 in mouse plasma.

J. Planar Chromatogr. 28, 398-401 (2015). TLC of pirfenidone in tablet formulation on silica gel with toluene - methanol 4:1. Quantitative determination by absorbance measurement at 315 nm. The hRF value for pirfenidone was 49. Linearity was in the range of 800-1600 ng/zone. LOD and LOQ were 0.550 and 1.667 ng/zone. The intermediate precision was below 1.8 % (n=3). Recovery ranged between 100 and 102 %.

J. Planar Chromatogr. 29, 121-126 (2016). HPTLC of caffeic acid (1), chlorogenic acid (2) and gallic acid (3) in plant-derived foods (organic cacao, green coffee, chia seeds, goji berries, spirulina, and chlorella) on silica gel in an automated multiple development chamber using ethyl acetate – water – formic acid 17:1:2. Quantification by densitometry at 366 nm. The hRF values for (1) to (3) were 94, 37 and 85, respectively. Linearity was in the range of 0.5-8.0 μg/zone for (1) and 0.5-10.0 μg/zone for (2) and (3). Free radical scavenging activity was assessed using the DPPH radical assay. For this purpose, the developed plates were sprayed with 0.4 % 2,2́-diphenyl-1-picrylhydrazyl in methanol.

Biochem. Biophys. Res. Commun. 470, 168-174 (2016). HPTLC of glycosphingolipid during the evaluation of enzyme activities of human Gb3/CD77 synthase supplemented with UDP-[14C]Gal on silica gel with chloroform – methanol – water 11:9:2. Detection of radiolabeled reaction products by exposure to phosphor screens for 8 weeks in -80 °C, followed by scanning. In parallel staining was used to facilitate identification of bands on the phosphor screens by spraying the plates with orcinol (0.2 %) in 3 M aqueous sulfuric acid, followed by heating at 110 °C for 10 min.

J. Planar Chromatogr. 29, 229-231 (2016). HPTLC of imipenem, meropenem, doripenem and eratapenem on silica gel G with 11 developing systems. Detection by spraying with iodine solution (0.2 g of potassium iodine and 0.4 g of iodine_x000D_ mixed in 20 mL of ethanol and 5 mL of hydrochloric acid), ninhydrin reagent (0.1 % ninhydrin in ethanol) or potassium permanganate solution (1 mg dissolved in 10 mL of water). The hRF values for the studied carbapenems in each developing system ranged between 13 and 58.