J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 583-584 (2004). TLC on silica gel with benzene - ethyl acetate - methanol - glacial acetic acid - water 16:10:8:4:3. Detection under daylight or UV light. Identification by finger print technique. TLC screening of many other animal's bile, such as duck bile, chicken bile, cow bile, sheep bile, pig bile and fish bile, etc. Discussion of the possibility of replacing snake bile with some other animal's bile containing similar components.

J. Liq. Chrom. Rel. Technol. 27, 1967-1980 (2004). Part of a study originally devoted to investigating lateral interactions of monocarboxylic and a,?-dicarboxylic acids analyzed on a low-activity stationary phase (cellulose powder) with low-polarity monocomponent mobile phases (decalin for monocarboxylic acids and 1,4-dioxan for a,?-dicarboxylic acids). TLC of lauric, myristic, palmitic, 3-phenylpropionic, 2-phenylbutyric, and 4-phenylbutyric acid with decalin and of succinic, adipic, and suberic acid with 1,4-dioxan on cellulose. Detection with an ethanolic solution of bromocresol green. Quantitative determination at 625 nm.

J. Planar Chromatogr. 17, 132-136 (2004). TLC and HPTLC of pravastatin, compactin and by-products (e. g. epi-pravastatin sodium salt and pravastatin-lactone) on silica gel in a twin-trough chamber with toluene - dichloromethane - tetrahydrofuran 23:1:1 for samples from ketone reduction and toluene - acetone - glacial acetic acid 20:30:4 for samples from compactin hydroxylation. Quantitative determination by reflectance measurement at 275 and 238 nm respectively.

J. Liq. Chrom. Rel. Technol. 27, 2047-2056 (2004). TLC of bromhexin on silica gel with n-butanol - glacial acetic acid - water 260:77:75 in a twin-trough chamber after at least 3 h of saturation. Quantitative determination at 325 nm by absorbance measurement. The method is selective, precise, and accurate and can be used for routine analysis of pharmaceutical preparations in pharmaceutical industry quality control laboratories.

J. Planar Chromatogr. 17, 449-453 (2004). In thin-layer radiochromatography the high sensitivity of the phosphor imaging analyzer can be further increased by multiple reading of the image plates, because the latent image is not lost quantitatively in the reading process. Summing of the chromatograms obtained in successive readings results in increased signal-to-noise ratio. Thus, by use of this approach either the exposure time can be shortened or higher sensitivity can be achieved. TLC of a14C-labeled test substance in rat serum on silica gel with chloroform - n-hexane - ethanol - ammonia 75:15:9:1. Detection by radioluminography.

J. Planar Chromatogr. 17, 360-364 (2004). Analytical OPLC of ascorbigen (ASC, 2-C-[(indol-3-yl)methyl]-alpha-l-threo-l-glycero-3-hexulofuranosonic acid lactone) on silica gel by means of two-step development: the first step (n-hexane) served for elimination of the total wetness front, the second (chloroform - methanol 9:1) for the separation. Detection by spraying with 10 mL Procházka’s reagent (reaction with formaldehyde), then heated for 5 min at 105 °C. Quantitative determination by densitometry at 460 nm.

IPC 56th 2004, Abstract No. G-20. Simultaneous HPTLC determination of loratadine and ambroxol in combined dosage form on silica gel with n-hexane - dichloromethane - triethanolamine 11:8:1.The Rf value of loratadine and ambroxal was found to be 0.40 and 0.16 respectively. Quantitative evaluation by scanning at 254 nm.The method was linear in the range of 0.2 -1 mg/spot for loratadine and 1.2 - 6 mg/spot for ambroxol with recovery of 98.2 - 98.5 %. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

IPC 56th 2004, Abstract No. GP-11. Stability indicating HPTLC determination of indapamide in tablets on silica gel with toluene - methanol 7:3. Quantitative determination by scanning at 246 nm. Optimization of experimental parameter such as band size, chamber saturation, and slit width. The method was linear in the range of 1.4 - 3.72 g, recovery was 100.01 %. The drug was subjected to stress conditions according to ICH guidelines, degradation products were separated from the pure drug. The method was validated for accuracy, precision, linearity, and specificity.