Abstract No. F-235, 61st IPC (2009). HPTLC of olanzapine on silica gel with methanol - ethyl acetate 4:1. The hRf value was 35. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 100-600 ng/band. The method was suitable for analysis of formulations and in-house prepared mucoadhesive microemulsions.

60th Indian Pharmaceutical Congress PG-264 (2008). HPTLC of quercetin in acetone extracts of Psidium guajava leaves on silica gel with toluene - acetone - formic acid 38:10:5. Quantitative determination of quercetin by absorbance measurement at 364 nm. The correlation coefficient was 0.9847. There was a good correlation between peak area and corresponding concentration of quercetin. The proposed HPTLC method provided a good resolution of quercetin from other constituents present in acetone extract of tender leaves of Psidium guajava and can be used for the quantification of quercetin.

J. Planar Chromatogr. 22, 287-291 (2009). HPTLC of the biomarkers gallic acid, lupeol, oleanolic acid, and stigmasterol and plant extracts on silica gel with toluene - ethyl acetate - formic acid 5:5:1 for gallic acid and with toluene - ethyl acetate 4:1 for lupeol, oleanolic acid, and stigmasterol in a saturated twin trough chamber. Quantitative determination by absorbance measurement at 272 nm. Detection of oleanolic acid, lupeol, and stigmasterol by dipping in anisaldehyde reagent followed by heating at 110 °C for 5 min. Densitometric evaluation at 652 nm.

J. Planar Chromatogr. 22, 293-296 (2009). TLC of verbascoside, forsythoside B, caffeoyl-malic acid and plant (Ballota nigra, B. hirsuta, and B. rupestris) extracts on silica gel with formic acid - acetic acid - water - ethyl acetate 15:15:36:134. Quantitative determination by fluorescence measurement at 395 nm. It was observed that amounts of phenylpropanoids in Ballota nigra leaves increase during the main and secondary flowering periods in June.

Bio. Chromatogr. 21(10), 1064-1068 (2008). Indirect chiral TLC separation of penicillamine (3,3-dimethylcysteine) enantiomers after derivatization with Marfey's reagent (FDNP-Ala-NH2) and two of its structural variants, FDNP-Phe-NH2 and FDNP-Val-NH2 on silica gel and RP-18 with phenol - water 3:1 and solvent combinations of acetonitrile and triethylamine phosphate buffer. The methods were applied for determination of the enantiomeric impurity of l-penicillamine, d-penicillamine, and pharmaceutical formulations of d-penicillamine.

Quim. Nova 33, 43-47 (2010). HPTLC of aflatoxin B1 in peanuts on silica gel with chloroform - acetone 99:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by image processing using the software ImageJ. Linearity was between 0.8 and 4.8 ng/zone. The intra-day and inter-day precisions had a RSD lower than 5.2 %. LOD was 0.4 ng/zone while LOQ was 1.2 µg/kg. The average recovery was 94.9 %. The proposed method is a simple, efficient and low cost tool for quantitative analysis of aflatoxin B1 in peanut samples.

J. AOAC Int. 93, 1617-1621 (2010). HPTLC of aconitine on silica gel with ethyl acetate - ethanol 3:1 at 22 °C in a saturated twin-trough chamber. The hRf value of aconitine was 33. Quantitative determination by absorption measurement at 238 nm. LOD and LOQ was 20 and 70 ng/band, respectively. The linearity with respect to peak area was in the range of 300 to 1800 ng/band with an r of 0.9991. The repeatability (RSD) was 0.85 %; and the inter-day and intra-day precision (RSD) was 1.01-1.38 and 1.04-1.34 %, respectively.

J. Planar Chromatogr. 23, 75-78 (2010). HPTLC of fluphenazine hydrochloride on silica gel (prewashed with methanol) with methanol - water 9:1 in a saturated twin-trough chamber. Quantitative determination by absorbance measurement at 306 nm. Linearity was in the range of 100 to 500 ng/µL with a correlation coefficient of 0.998. LOD and LOQ were 1.45 and 4.40 ng/zone, respectively. Intra-assay and inter-assay precision, expressed as relative standard deviation (RSD), were in the range 0.73-1.77 % (n = 3) and 1.18-1.86 % (n = 9), respectively. Recovery of fluphenazine hydrochloride was between 98.3 and 101.5 %, with RSD not higher than 1.87 %. The method was selective for fluphenazine hydrochloride and the preservatives in the injections.