Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      109 108
      High-performance thin-layer chromatographic determination of etoricoxib and thiocolchicoside in combined tablet dosage form
      V.S. RAJMANE, S.V. GANDHI*, U.P. PATIL, M.R. SENGAR (*A.I.S.S.M.S. College of Pharmacy, Department of Pharmaceutical Analysis, Kennedy Rd, Near R. T. O., Pune-411 001, Maharashtra, India; cantoshvgandhi@rediffmail.com)

      J. AOAC Int. 93, 783-786 (2010). HPTLC of etoricoxib (ETO) and thiocolchicoside (THIO) on silica gel (prewashed with methanol) with ethyl acetate - methanol 4:1 in a twin-trough chamber after preconditioning for 20 min. Quantitative determination by absorbance measurement at 290 nm. The calibration curve was linear over a range of 50-250 and 100-500 ng/band with correlation coefficients of 0.9948 and 0.9958 for ETO and THIO, respectively. LOD and LOQ were 11 and 33 ng/band for ETO and 25 and 76 ng/band for THIO. %RSD values were found to be 0.5 and 0.8 % for ETO and THIO for intra-day variations, while inter-day variations were 1.2 and 1.0 %, respectively. The recovery for ETO was between 100.2-101.1 % and for THIO 98.7-100.4 %.

      Classification: 32a
      109 132
      Analysis of sulfide ions by densitometric thin-layer chromatography and use of TLSee software
      W. CIESIELSKI, K. DYNSKA-KUKULSKA, R. ZAKRZEWSKI*, A. HEKNER (*University of Lódz, Faculty of Chemistry, Department of Instrumental Analysis, ul. Pomorska 163, Lódz, Poland; robzak@chemia.uni.lodz.pl)

      J. Planar Chromatogr. 23, 343-347 (2010). TLC and HPTLC of (derivatized) sulfide ions on silica gel with dichloromethane - methanol - diethyl ether - 25 % ammonia 40:5:5:1 in a horizontal chamber saturated for 20 min. Detection by using the methylene blue method, i.e. production of methylene blue by oxidative coupling of sulfide with N,N-dimethyl-p-phenylenediamine in the presence of iron(III) ions in acidic medium. Quantitative determination by absorbance measurement at 660 nm (method I), or analysis by TLSee software (method II). Response for both was a linear function in the concentration range of 20-100 pmol/zone. The LOD and LOQ were 10 pmol/zone (3.2 mg/L) and 20 pmol/zone (6.4 mg/L). The repeatability of both methods (%RSD) was 1.9-4.2 % for method I and 1.5-3.9 % for method II.

      Classification: 33b
      110 041
      Qualitative and quantitative analysis of hyaluronan oligosaccharides with high-performance thin layer chromatography using reagent-free derivatization on amino-modified silica and electrospray ionization-quadrupole time-of-flight mass spectrometry couplin
      M. ROTHENHÖFER, ROSMARIE SCHERÜBL, G. BERNHARDT, J. HEILMANN*, A. BUSCHAUER (*Lehrstuhl für Pharmazeutische Biologie, Universität Regensburg, Universitätsstr. 31, 93040 Regensburg, Germany)

      J. of Chromatogr. A 1248, 169-177 (2012). Purified oligomers of hyalobiuronic acid are indispensable tools to elucidate the physiological and pathophysiological role of hyaluronan degradation by various hyaluronidase isoenzymes. Establishment and validation of a novel sensitive, convenient, rapid, and cost-effective HPTLC method for the qualitative and quantitative analysis of small saturated hyaluronan oligosaccharides consisting of 2–4 hyalobiuronic acid moieties. HPTLC on amino phase with 1-butanol - formic acid - water 3:5:2 or 3:4:1. Detection 1) by spraying with orcinol in various concentrations of sulfuric acid; 2) by dipping into the reagent of orcinol in 10 % sulfuric acid and Morgan–Elson reagent; 3) by illuminating with white light and UV 366 nm after heating. The simple reagent-free in situ derivatization of 3) resulted in a detection limit of 7–19 pmol/band and LOQ of 37–71 pmol/band depending on the analyzed saturated oligosaccharide. Identification of the analytes by TLC-ESI-MS. The validated HPTLC method, as an alternative to sequential techniques such as HPLC and CE, can easily be automated and is applicable to the analysis of multiple samples in parallel.

      Classification: 17
      110 063
      A validated stability-indicating HPTLC method for the estimation of gemcitabine HCl in its dosage form
      S. BORISAGAR, H. PATEL*, C. PATEL (*Department of Quality Assurance, Shree Sarvajanik Pharmacy College, Nr. Arvind Baug, Mehsana 384001, Gujarat, India, harshaupatel@yahoo.co.in)

      J. Planar Chromatogr. 25, 77-80 (2012). HPTLC of gemcitabine on silica gel with toluene - methanol - chloroform 6:6:5. Quantitative determination by absorbance measurement at 268 nm. The hRf value of gemcitabine was 48. Linearity was in the range of 500-3000 ng/band. Intraday and interday precisions (%RSD) were 1.4-1.6 % and 1.6-1.8 %, respectively. Recovery was found to be in the range of 98.3-101.8 %.

      Classification: 32a
      110 107
      Simultaneous HPTLC analysis of atorvastatin calcium, ezetimibe, and fenofibrate in tablet
      C. MACWANA*, A. PATEL, V. PARMAR, S. PATEL (*Institute of Pharmaceutical Education and Research, Gh-6, Sec 23, Gandhinagar, India, chhayamacwana111@gmail.com)

      J. Liq. Chromatogr. Relat. Technol. 35, 524-532 (2012). HPTLC of atorvastatin calcium (1), ezetimibe (2), and fenofibrate (3) in tablet on silica gel with toluene – chloroform – methanol 23:15:7 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 253 nm. The hRf values of active agents (1) - (3) were 20, 33 and 80, respectively. Linearity was 200-800 ng/zone for (1) and (2) and 4-16 µg/zone for (3). The intermediate/inter-day/intra-day precision was below 1.1 % for (1), 1.3 % for (2) and 1.5 % for (3) (n=6). The limits of detection and quantification were 19 and 59 ng/zone for (1), 23 and 68 ng/zone for (2), and 1449 and 4390 ng/zone for (3), respectively. Recovery (by standard addition) was between 99.1 and 99.8 % for compounds (1) to (3).

      Classification: 32a
      110 128
      Application of stability indicating high-performance thin-layer chromatographic method for quantitation of desvenlafaxine in pharmaceutical dosage form
      S. PAWAR, S. DHANESHWAR* (*Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India 411038, sunil.dhaneshwar@gmail.com)

      J. Liq. Chromatogr. Relat. Technol. 35, 499-510 (2012). HPTLC of desvenlafaxine in dosage forms on silica gel with ethyl acetate - toluene - methanol 14:4:1 + 1 drop ammonia. Quantitative determination by absorbance measurement at 228 nm. The hRf value of desvenlafaxine was 48 and selectivity regarding matrix was given. Linearity was between 100 and 1000 ng/spot. The intermediate/inter-day/intra-day precision was below 0.2 % (n=6). The limits of detection and quantification were 10 and 100 ng/spot, respectively. Recovery (by standard addition) was between 97.9 and 99. 1%.

      Classification: 32a
      110 169
      Validated HPTLC method for simultaneous determination of loratadine and desloratadine in presence of co-formulated drug
      R. YOUSSEF*, E. KHAMIS, M. EL-SAYED, Mona MONEIM (*Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, University of Alexandria, El-Messalah, Alexandria 21521, Egypt, rmmy1973@yahoo.com)

      J. Planar Chromatogr. 25, 456-462 (2012). HPTLC of loratadine (1) and desloratadine (2) on silica gel with methanol + 1 drop ammonia. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) and (2) were 76 and 20, respectively. Linearity was in the range of 250-850 ng/band for (1) and 100-1000 ng/band for (2). Limits of detection and quantification were 90 and 250 µg/band for (1) and 30 and 100 µg/band for (2), respectively. Intra- and inter-day precisions were found to be less than 2 %. Recoveries were between 97.8 and 101.5 % for both (1) and (2).

      Classification: 32a
      111 030
      Quantitative determination of steviol glycosides (Stevia sweetener)
      Stephanie MEYER, Gertrud MORLOCK* (*Justus Liebig University of Giessen, Institute of Nutritional Science, IFZ, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, gertrud.morlock@ernaehrung.uni-giessen.de)

      CBS 109, 10-12 (2012). HPTLC of steviol glycosides (stevioside, rebaudioside, dulcoside A, steviolbioside) on silica gel (pre-washed with methanol and dried at 100 °C for 15 min) with ethyl acetate - methanol - acetic acid 3:1:1 over 60 mm. Detection under white light after immersion in ß-naphthol reagent (2 g in 180 mL ethanol with 12 mL 50 % sulfuric acid) and heating at 120 °C for 5 min. Quantitative absorption measurement at 500 nm after derivatization. LOD was 10 ng/band and LOQ 30 ng/zone. Using the calibration curve method the LOQ was reduced to 12 ng/band via peak height and 20 ng/band via peak area. The calculated expected tolerance range over the whole procedure inclusive sample preparation considered recovery rates at 3 different concentration levels (0.02, 0.13, and 0.20 %) in milk-based matrix. The accuracy (recovery tolerance limit of 92-120 %), repeatability (3.1-5.4 %) and intermediate precision (4.0-8.4 %) were highly satisfying, exemplarily shown for stevioside in milk-based matrix. ANOVA was successfully passed to prove the working range. With the newly developed and validated HPTLC method, steviol glycosides in Stevia leaves, Stevia formulations, and food products were investigated.

      Classification: 14
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