J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (3), 272-275 (2005). TLC of the extracts on silica gel with 1) n-hexane - chloroform - methanol 15:8:2; 2) n-hexane - ethyl acetate 9:1; 3) chloroform - benzene - glacial acetic acid 7:2:1. Detection 1) by exposing to iodine vapours; 2) under UV 365 and 254 nm. Identification by comparison with the standard. Quantification of usnic acid by densitometry at 290 nm. Validation by investigating the linearity range (0.50 - 2.50 µg/spot, r = 0.999), precision (RSD = 2.26 %, n= 5, within plate, and 2.97 %, n = 5, plate-to-plate), and standard addition recovery (97.5 %, RSD = 2.61, n = 5).

Indian Drugs 42 (12), 805-807 (2005). HPTLC of alprazolam and sertraline in tablet dosage form on silica gel with toluene - ethyl acetate - methanol - acetic acid 90:30:20:3 without saturation. Quantitative determination by absorbance measurement at 217 nm. Accuracy, precision, and linearity were established. The linearity range was 20-100 ng for alprazolam and 100-500 ng for sertraline. Recovery rates were between 99.8-100.5 % for both drugs.

J. Planar Chromatogr. 19, 167-170 (2006). HPTLC of standards (cholesterol, oleic acid, triolein, methyl oleate, and cholesteryl oleate as marker compounds for free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters, respectively, as well as cholesterol, phosphatidylethanolamine, phosphatidylcholine, and lysophosphatidylcholine for the analysis of polar lipids) and prepared samples of leeches on silica gel (with preadsorbent zone and prescored lanes) in a pre-saturated twin-trough chamber with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 (for neutral lipids) and hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1 (for steryl esters). Visualization by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 110 °C. HPTLC of polar lipids on equal layer with chloroform - methanol - water 65:25:4. Visualization by spraying with 5% aqueous cupric sulfate solution and heating for 10 min at 140 °C. Quantification of neutral lipids at 610 nm and of polar lipids at 370 nm.

Abstract GP-55, IPC (2005). HPTLC of methanolic extracts of ambroxol and cetirizine combination tablets, on silica gel with methanol - ethyl acetate - toluene - ammonia 40:15:56:10 drops of ammonia. Quantitative determination by absorbance measurement at 231 nm. Rf values of cetirizine was 0.40 and of ambroxol 0.78, linearity range was 0.4-0.8 µg for cetirizine and 4-10 µg for ambroxol. Recovery was 99.3-100.4 % for both compounds. In comparison with a spectrophotometric method the HPTLC method had the advantage of higher throughput.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 854-856 (2005). TLC of chenodeoxycholic acid in Hedan tablets on silica gel with n-hexane - ethyl acetate - acetic acid - methanol 20:25:2:3. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots are visualized. Quantification of chenodeoxycholic acid by densitometry at 375 nm. Validation of the procedure by investigation of its linearity range (0.47 - 2.33 µg/spot, R = 0.9992); of its repeatability (3.3 %, n = 5); of its precision (3.9 %, n = 5 within plate and 4.7 % n= 5 plate-to-plate); and its standard addition recovery (98.4%, RSD = 2.5 %, n = 5).

Indian Drugs 42 (9), 600-603 (2005). HPTLC of cinnarizine and domperidone in tablets, on silica gel with toluene - ethyl acetate - methanol 14:1:5. Quantitative determination by absorbance measurement at 271 nm. Rf values of cinnarizine was 0.85 and of domperidone 0.4. Linearity was observed in the range of 0.1-0.4 for cinnarizine and 0.075-0.3 µg/µL for domperidone. The recoveries were in the range of 98.95-100.25 %. The tablet matrix did not interfere with the assay.

Acta Chrom. 9, 79-86 (1999). HPTLC of the primary carbohydrates in the planorbid snail Biomphalaria glabrata on silica gel CF254 with acetonitrile – water 17:3 and ethyl acetate – acetic acid – methanol – water 12:3:3:2, on LK5DF silica gel with ethyl acetate – acetic acid – methanol – water 12:3:3:2, on RP-18 with tetrahydrofuran – water 22:3, on cellulose with ethyl acetate – pyridine – water 2:1:2 and on amino-bonded layers with ethyl acetate – pyridine – water – acetic acid 12:6:2:1. The use of specific detection reagents for reducing sugars (4-aminobenzoic acid detection reagent and aniline – DPA reagent), spiking experiments, and spraying with alpha-naphthol – sulfuric acid reagent for quantitative analysis (densitometry at 515 nm) aided the identification of glucose and maltose. GC-MS analysis confirmed the identification of maltose and glucose on the basis of retention times and spectral fingerprints. A starvation study was conducted to determine changes in sugar levels in B. glabrata digestive gland–gonad complex (DGG) and hemolymph samples during a 12-day starvation period.

J. Planar Chromatogr. 19, 443-448 (2006). HPTLC of diclofenac sodium and paracetamol (with aceclofenac as internal standard) on silica gel, pre-washed with methanol, in a presaturated twin-trough chamber with toluene - ethyl acetate - methanol - formic acid 50:40:10:1. Quantitative determination by absorbance measurement at 260 nm. The method was validated regarding accuracy and precision.