Abstract G-25, IPC (2005). HPTLC of cefuroxime axetil in bulk and tablets on silica gel with chloroform - methanol - toluene 2:1:1 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 290 nm. The linearity was within the range of 300-900 ng/spot with an average recovery rate of 99.4 %. The method was validated as per ICH guidelines.

J. Planar Chromatogr. 18, 160-163 (2005). TLC of rare earths (La(III), Ce(III), Pr(III), Sm(III), Gd(III), Er(III)) on silica gel and silica gel impregnated with 2.5 M ammonium nitrate with different mixed mobile phases containing di(2-ethylhexyl)dithiophosphoric acid as a complexing agent. The best results were obtained by use of ethyl methyl ketone - tetrahydrofuran - 1 M di(2-ethylhexyl)dithiophosphoric acid 17:8:1. Double development was used to obtain better separation of consecutive rare earths.

J. Planar Chromatogr. 19, 187-190 (2006). Comparison of the iridoid composition of ten Stachys species by use of a TLC-densitometric method. TLC of harpagide, acetylharpagide, harpagoside, ajugoside, aucubin, and catalpol on silica gel with chloroform - methanol - water 25:10:1 and, occasionally, ethyl acetate - formic acid - water 9:2:1. Visualization of the spots by use of Ehrlich’s spray reagent (1 % dimethylaminobenzaldehyde in concentrated hydrochloric acid) followed by heating at 105 °C for 5 min. Quantitation by densitometry at 540 nm after 3 h.

J. AOAC Int. 89, 619-623 (2006). HPTLC of phyllanthin, hypophyllanthin, gallic acid, and ellagic acid on silica gel with toluene - ethyl acetate - formic acid 6:2:1 at 25 +/- 2 °C and 40 % relative humidity. Quantitation by densitometry at 280 nm. The method was validated for precision (0.54, 0.93, 0.08, and 1.06 %, respectively), repeatability (1.01, 0.79, 0.98, and 1.06 %, respectively), and accuracy, determined by a recovery study at 3 different levels (99.09 %, 99.27 %, 98.69 %, and 100.49 %, respectively).

Abstract GP-74 IPC (2005). HPTLC of etoricoxib in tablets on silica gel with toluene - 1,4 dioxane - methanol 17:2:1. Quantitative determination by absorbance measurement at 235 nm. Linearity range was 500-1500 ng/spot. For stability studies the sample was subjected to acid and alkali hydrolysis, thermal, oxidaline and photo degradation. The peaks of all the degraded products were well resolved with significant different Rf values. The method was validated for different parameters and found suitable for routine quality control.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 783-785 (2005). TLC of ecdultin in Bazi Bushen capsules on silica gel with benzene - ethyl acetate 30:1. Detection under UV 365 nm. Quantitative determination of ecdultin by fluorescence measurement at 320 nm. Validation of the procedure by investigation of the optimum excitation wavelength; linearity range (0.022 - 0.13 µg/spot, R = 0.9998); repeatability (1.5 %, n = 6); precision (0.87 %, n = 6 within plate and 1.42 %, n= 6 plate-to-plate); and standard addition recovery (98.7 %, RSD = 1.83 %, n = 6). The results for six batches of real life samples are given.

Mini review and chromatographic-computional analysis. Acta Chrom. 17, 9-19 (2006). Comparative correlative TLC analysis conducted on whole-tissue homogenate of control and in-vitro-incubated samples of mammalian myocardia and bullfrog cardiac muscle and thigh skeletal muscle. The focus in the study was on TLC-densitometric analysis of the endogenous cardiolipin of control samples and their respective incubated samples to identify the products, e. g. lysocardiolipin, formed during in-vitro incubation. TLC of the chloroform-methanol extracts (2:1) obtained after the incubation from the freeze-dried tissues homogenate on silica gel with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18. Visualization with thionine reagent, densitometry at 600 nm.

C. Cheng by thin-layer chromatography) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 25 (6), 719- 721 (2005). TLC of fulvotomentoside A on silica gel with chloroform - methanol - water 61:32:5. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC for 5 min. Quantitative determination by densitometry at 518 nm. The procedure was validated regarding linearity range (1.20 - 7.20 µg/spot, r = 0.998), repeatability (0.51 %, n = 5), precision (0.21 %, n = 5 within plate and 0.87 % n= 5 plate-to-plate), and recovery (99.7 %, RSD = 2.45 %, n = 5). The results are given for three batches of real life samples.