J. Planar Chromatogr. 25, 156-161 (2012). HPTLC of haloperidol (1) and metabolites I (2), II (3) and III (4) in plasma on RP-18 with methanol - 0.001 % diethylamine. Quantitative determination by absorbance measurement at 230 nm. The hRf values of haloperidol and metabolites (2) to (4) were 22, 6, 16, and 87, respectively. Recoveries for (1) to (4) were 85, 88, 87, and 77 %, respectively. LOD for haloperidol was 1.0 mg/mL and for all the three metabolites (2) to (4) it was 0.8 mg/mL.

J. Liq. Chromatogr. Relat. Technol. 36, 2446-2462 (2013). HPTLC of amitriptyline in tablets on silica gel with acetone - ammonium hydroxide 99:1. Quantitative determination by absorbance measurement at 254 nm. The hRf value of amitriptyline was 46. Linearity was 0.70-1.30 µg/zone. The earlier model TLC to HPTLC-densitometry transfer procedure was expanded by adding sample peak identification confirmation and peak purity (specificity) tests.

J. Anhui Med. & Pharm. 17 (2), 202-203 (2013). Banlangen granule, processed of Radix Isatidis, is a traditional Chinese herbal medicine prescribed clinically to treat sore throat, cheek swelling, acute and chronic tonsillitis and mumps. For quality control a method for the determination of leucine, a main antiviral effective component, is presented. TLC on silica gel with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C and viewing in daylight. Quantification by densitometry at 509 nm. Validation of the method by investigation of the linearity range (0.10-0.90 µg/zone, r = 0.998, n=5), precision (%RSD = 2.8 %, n=6), stability (%RSD = 0.8 %, n=6, towards single lane within one hour), and reproducibility (%RSD = 2.0 %, n=6, within plate). The recovery (by standard addition) was 98.8 % (%RSD = 1.1 %, n=6).

J. Planar Chromatogr. 26, 306-311 (2013). HPTLC of baicalein (1), hispidulin (2), chrysin (3), and oroxylin A (4) in different plant parts of Oroxylum indicum on silica gel with chloroform - methanol 97:3 + 1 drop water + 1 drop formic acid. Quantitation by absorbance measurement at 270 nm. The hRf values for compounds (1) to (4) were 22, 2, 36 and 61, respectively. Linearity was in the range of 500-2500 ng/zone for (1) to (4). LOD and LOQ were 77 and 257 ng/zone for (1), 99 and 330 ng/zone for (2), 69 and 229 ng/zone for (3) and 45 and 151 ng/zone for (4), respectively. Average recoveries were 98.3 % for (1), 95.2 % for (2), 100.2 % for (3) and 98.0 % for (4). Intermediate/interday/intra-day precision was below 5 % (n=5).

species by validated HPTLC–densitometric method. J. Planar Chromatogr. 26, 248-253 (2013). HPTLC of ardisicrispin A in the roots, stems, leaves, fruits and flowers of Lysimachia nemorum L. (1), Lysimachia nummularia L. (2), Lysimachia vulgaris L. (3), Lysimachia punctata L. (4), Lysimachia thyrsiflora L. (5), Lysimachia ephemerum L. (6), Lysimachia ciliata L. (7), and Lysimachia clethroides Duby (8) on silica gel with chloroform - methanol - water 8:7:1 for (1), (3) and (7) and n-butanol - acetic acid - water 6:1:3 for (2), (4), (5), (6) and (8). Detection by spraying with 25 % sulfuric acid in methanol, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 545 nm. The hRf values for ardisicrispin A in both development systems were 86 and 36, respectively. Linearity was in the range of 2-13 µg/zone. LOD and LOQ were 620 and 950 ng/zone for the chloroform mobile phase and 1890-2880 ng/zone for the n-butanol mobile phase. Average recovery was in the range of 94.3-95.1 %. Intermediate/interday/intra-day precision was below 4 % (n=3).

J. Planar Chromatogr. 26, 43-55 (2013). TLC of cefditoren pivoxil on silica gel with 1-butanol - acetic acid - water 17:2:1. Quantitative determination by absorbance measurement at 295 nm. The hRf of cefditoren pivoxil was 70. Linearity was in the range of 600-1600 ng/zone. The LOD and LOQ were 40 and 130 ng/zone, respectively. Recovery (by standard addition) was between 97.8 and 101.8 %. Intermediate/interday/intra-day precision was below 2 % (n=3). The method showed comparable results with a validated HPLC method.

J. Planar Chromatogr. 26, 435-439 (2013). HPTLC of ciclesonide in bulk and dosage form on silica gel with acetonitrile - 1,4-dioxane - n-hexane 1:1:8 +1 drop ethyl acetate. Quantitative determination by absorbance measurement at 242 nm. The hRf of ciclesonide was 38. Linearity was between 1 and 6 mg/zone. LOD and LOQ were 100 and 303 ng/zone. Recovery (by standard addition) was found to be 98.9-100.3 %. Intra- and inter-day precision values were below 0.7 and 1.4 %, respectively.

J. Planar Chromatogr. 27, 174-180 (2014). HPTLC of betulinic acid (1), 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (2) and lupeol (3) in Clerodendrum phlomidis on silica gel with chloroform - methanol 49:1. Detection by dipping into vanillin - sulfuric acid reagent (vanillin - ethanol - sulfuric acid 1:95:5, w/V/V), followed by heating at 110 ºC for 3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (3) were 30, 52 and 70. Linearity was in the range of 300-1500 ng/mL. The intermediate/interday/intra-day precisions were below 2 % (n=5). The LOD and LOQ were 22 and 74 ng/zone for (1), 20 and 66 ng/zone for (2) and 30 and 100 ng/zone for (3), respectively. Average recovery was 99.0 % for (1), 98.5 % for (2) and 98.2 % for (3).