Anders. J. Planar Chromatogr. 31, 437-443 (2018). HPTLC of apigenin (1) and luteolin (2) in Hygrophila spinosa on silica gel with toluene – ethyl acetate – formic acid 6:4:1. Quantitative determination by absorbance measurement at 349 nm. The hRF values for (1) and (2) were 64 and 54, respectively. Linearity ranged between 80-560 ng/zone for (1) and 40-280 ng/zone for (2). LOD and LOQ were 6 and 19 ng/zone for (1) and 2 and 7 ng/zone for (2), respectively. The intermediate precision was <3 % (n=3). Recovery was between 99.3 and 100.5 % for (1) and 99.5 and 100.8 % for (2).

Phytochem. Anal. 30, 121-131 (2019). HPTLC bioautography of essential oils of oregano, rosewood and cumin on silica gel with ethyl acetate – petroleum ether 1:19 for cumin, ethyl acetate – petroleum ether 1:9 for oregano and ethyl acetate – petroleum ether 1:3 for rosewood. 2D resolution by eluting for a second time along the orthogonal axis. TLC-direct bioautography by spraying with a bacterial suspension of methicillin‐sensitive S. aureus NCTC 1298 (MSSA), methicillin‐resistant S. aureus NCTC 12497 (MRSA), E. coli NCTC 8003, ciprofloxacin‐resistant E. coli (clinical isolate), P. aeruginosa NCTC 6749, P. aeruginosa (clinical isolate), vancomycin‐sensitive Enterococcus faecium and vancomycin‐resistant E. faecium NCTC 12202, followed by incubation at 37 ºC for 4 h. Antimicrobial fractions were further characterized by NMR spectroscopy, GC-MS and LC-MS.

Proc. Intern.Symposium on TLC with special emphasis on OPLC, Szeged, 107 (1984). OPLC (OPTLC) of a dye mixture on alumina with toluene.

J. Chromatogr. 292, 479-484 (1984). HPTLC of heterogeneous mixtures of dansylated ovalbumine glycopeptides incubated with rat brain homogenates on silica with methyl acetate - chloroform - propanol - methanol - 0.25 % aq. potassium chloride 25:20:20:20:17. Prior to separation, dansyl-L-leucine: is added as internal standard. Fluorescence scanning at 366/>400 nm.

J. Chromatogr. 292, 117-127 (1984). HPTLC of phenylurea herbicide hydrolysates and their aniline metabolites on silica with dichloromethane or dichloromethane - methanol 99:1 and on RP-18 with acetonitrile -3 % aq. sodium chloride solution 85:15. Prior to development, the applied spots were overspotted with 4 mL of 0.25 DNS chloride solution. An increased fluorescence intensity was obtained by dipping the plates into paraffin - hexane 2:1. Scanning by fluorescence, excitation at 360 nm. Detection limit 1 ng.

R.E. Kaiser (Ed) Proc. of the 3rd Int. Symp. on Instr. HPTLC, IfC, Bad Duerkheim 1985, 475-488. Emphasis on influence of the noise caused by the particles and mechanical defects (structure) of the layer on quantitative TLC analysis. Improvement of accuracy and repeatability by total area quantification prior and after the chromatographic step of TLC cross correlation of quantitative and qualitative data.

(Hungarian) (Data on the thermo and light stability of the receptorblocking agent CH-103.) CH-103 = (4-cyclohexylamino-1-(1-naphtyloxy)-2-butanol-hydrogenmaleate) is a new Hungarian pharmacon. TLC on silica with ethyl acetate - methanol -25 % NH3 8:1:1 Detection by UV and with iodine. Spectrophotometry after elution. Main decomposition products identified: 1-naphtol, 4-cyclohexylamine-1,2-butandiol, 1-cyclohexenyl-3-pyrrolidinol.

Chem.) 34, 707-711 (l985). (Japanese) (Determination of higher fatty acids in phospholipid subfractions of human platelets using thin-layer chromatography and gas chromatography.) TLC of phospholipid subfractions on silica with chloroform - methanol acetic acid - formic acid 50:30:4.5:6.5. Detection by spraying with 8-anilinonaphtalenesulfonic acid ammonium salt. Quantification by GC after extraction and methylation. Phospholipids were extracted from silica with chloroform - methanol 1:4 once and methanol twice. Methylation was done with boron trifluoride and methanol. The method is highly reliable.