Pharmacophore 1(2), 103-111 (2010). HPTLC of wedelolactone in powdered dried aerial parts of Eclipta alba Hassk, extracted with methanol, on silica gel with toluene – ethyl acetate – formic acid 50:50:1. Quantitative determination by absorbance measurement at 351 nm.

CBS 105, 10-12 (2010). HPTLC of stratum corneum lipids (ceramides, cholesterol, phosphatidylcholine, squalene, sphingomyelin etc.) on silica gel by automated multiple development with a 8-step gradient from methanol to hexane in the AMD2 with pre-conditioning with 4M acetic acid before step 6. Detection by immersion in copper(II)sulfate reagent followed by heating at 170 °C for 8 min. Quantitative determination by absorbance measurement at 600 nm. Phosphatidylcholine and sphingomyelin remain at the start position, all other substances are separated.

62nd Indian Pharmaceutical Congress Abstract No. F-264 (2010). TLC of lamivudine, zidovudine and nevirapine on silica gel with chloroform – methanol 9:1. The hRf values were 7, 46 and 77 for lamivudine, zidovudine and nevirapine, respectively. Quantitative determination by absorbance measurement at 265 nm. The method was linear in the range of 90-210 µg/band, 180-240 µg/band and 120-280 µg/band for lamivudine, zidovudine and nevirapine respectively.

Der Pharma Chemica 2(3), 8-18 (2010). HPTLC of berberine on silica gel with methanol – acetic acid – water 8:1:1. The band corresponding to berberine showed an hRf value of 74. Quantitative determination by absorbance measurement at 350 nm. The method was linear in the range of 100-500 ng/band. Different samples analysed by the proposed method were found to contain 11.8-12.5 mg/g berberine. The recovery was between 98.0-100.3 %.

Acta Chromatographica 20(3), 463-473 (2008). TLC on silica gel with toluene – methanol – triethylamine 35:15:1. The hRf of atenolol and lercanidipine hydrochloride was 24 and 68, respectively. Detection and quantitative determination by absorbance measurement at 275 nm. The linearity was in the range of 2-12 µg/band for atenolol and 400–2400 ng/band for lercanidipine hydrochloride. The recovery was 98.9 % for atenolol and 99.7 % for lercanidipine hydrochloride.

Acta Chromatographica 22 (2), 259-265 (2010), DOI:10.1556/AChrom.22.2010.2.8. Description of a new, simple, precise, and accurate method for quantification of (-)-epicatechin in the leaves of Cassia fistula by HPTLC on silica gel with toluene – ethyl acetate – formic acid – methanol 205:3:1:1. Quantification by densitometry at 280 nm. The linearity was in the range of 200–800 ng/band. Method precision was 1.4 %RSD and instrumental precision 1.1 %RSD. Recovery was 98.1 % and specificity regarding matrix was given.

J. of Food Sci. & Biotechnol. 27(5), 129-133 (2008). TLC of gentamicin on silica gel with the lower phase of chloroform – methanol – 25 % ammonia 5:4:3 and after chamber saturation with the upper phase of the developing solvent. Detection by exposure to iodine vapor. Identification by comparison of the hRf values with the standards of the main components of gentamicin (Cl, C1 and C2). The results were compared with results obtained by HPLC and good agreement between both methods was found.

Rapid Commun. Mass Spectrom. 25, 2275-2282 (2011). The authors explored the possibility of the desorption at an angle scanning analysis of surfaces from direct analysis in real time mass spectrometry (DART-MS), including the coupling of planar chromatography with DART-MS. A method for the visualization of the impact region of the DART gas stream was developed, as well as the DART-MS detectability of liquids was increased, improving the capabilities of DART-MS in trace analysis.