J. Chromatogr. A 1216 (42), 7102-7107 (2009). HPTLC of co-trimoxazole tablets (combination of sulfamethoxazole and trimethoprim) on silica gel with toluene – ethyl acetate – methanol 100:57:43. Detection under UV 254 nm. The hRf values were 30 and 61 for trimethoprime and sulfamethoxazole, respectively. Quantification by densitometry at UV 254 nm. Cochran's criterion test indicated homoscedasticity of variances for the calibration data. The F-tests for lack-of-fit indicated that straight lines were adequate to describe the relationship between spot areas and concentrations for each compound. Repeatability and precision (%RSD) was 1.0 and 0.8 % for sulfamethoxazole as well as 1.3 % and 1.6 % for trimethoprim, respectively. Recovery was 99.0 % and 99.7 % for sulfamethoxazole and trimethoprim, respectively.

Abstract No. F-347, 61st IPC (2009). HPTLC of gatifloxacin and ornidazole on silica gel with dichloromethane - methanol - 25 % ammonia 95:10:3. The hRf value was 16 and 60 for gatifloxacin and ornidazole, respectively. Quantitative determination by absorbance measurement at 302 nm. The method was linear in the range of 20-200 ng/band for gatifloxacin and 50-500 ng/band for ornidazole. Recovery was between 100.4 and 101.9 for both drugs.

60th Indian Pharmaceutical Congress PG-244 (2008). HPTLC of Centella asiatica extract with asiaticoside as marker on silica gel with chloroform - glacial acetic acid - methanol - water 15:8:3:2 with chamber saturation. Detection by spraying with anisaldehyde reagent, followed by heating in oven and immediate quantitative determination by absorbance measurement at 607 nm. The hRf value of asiaticoside was 81. Linearity was in the range of 0.96-3.36 µg/spot. Hydroalcoholic extracts contained approx. 3.2 % of asiaticoside.

60th Indian Pharmaceutical Congress PA-218 (2008). TLC of aceclofenac and paracetamol on silica gel with toluene - isopropylalcohol - ammonia 8:7:1. The hRf value of aceclofenac was 24 and of paracetamol 68. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 25-2000 ng/band with correlation coefficients of 0.9998 for aceclofenac and 0.9996 for paracetamol. The limits of detection and quantification were 25 and 150 ng/zone for aceclofenac and 50 and 200 ng/zone for paracetamol. Both drugs were subjected to acid and alkali hydrolysis, oxidative degradation, and photodegradation. The degradation products were well resolved from the pure drug.

Abstract No. 9966, IHCB (2009). HPTLC of root tuber extracts of Asparagus gonoclados Baker on silica gel with ethyl acetate - methanol - water 15:3:2. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 425 nm. Shatavarin IV was used as marker.

Ind. J. Pharma. Sci. 8(4), 211-212 (2009). TLC of clopidogrel bisulphate on silica gel with carbon tetrachloride - ethyl acetate - ammonia 50:3:2. Quantitative determination by absorbance measurement at 230 nm. Linearity was between 300 and 1500 ng. In comparison with the labeled claim the amount of clopidogrel in tablets was 99.2 %. The recovery was 99.2 % (via peak area).

60th Indian Pharmaceutical Congress PA-192 (2008). HPTLC of strychnine and piperine in herbal extracts and herbal formulations on silica gel with toluene - ethyl acetate - diethyl amine 7:2:1. Quantitative determination by absorbance measurement at 283 nm. Linearity was 400-2000 ng/spot, recovery was in the range of 99.5-101.0 both for strychnine and piperine.

Abstract No. F-261, 61st IPC (2009). HPTLC of quetiapine on silica gel with methanol - toluene 4:3. The hRf value was 41. Quantitative determination by absorbance measurement at 235 nm. The method was linear in the range of 100-500 ng/band. Recovery was 98.9 %.