Ham. Ex D. Don, Myricaceae. J. Planar Chromatogr. 23, 326-331 (2010). HPTLC of myrecitin and stem bark extracts on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:3:2. Quantitative determination by absorbance measurement at 268 nm. Linearity was between 0.4-2.0 µg/band. The limits of detection and quantitation were 93 ng and 284 ng/zone. The intra-day and inter-day precision of the method was in the range of 0.14-0.55 %. The recovery of myrecitin at three concentrations was in the range of 98.9-100.1 % and the average recovery was 99.3 %.

J. Planar Chromatogr. 24, 406-411 (2011). HPTLC of plant extracts and vasicine on silica gel with ethyl acetate - methanol - ammonia (17.3 % ammonia) 40:10:1 in a twin-trough chamber saturated for 15 min. Quantitative determination by densitometry at 298 nm. The hRf value of vasicine was 47. The limit of detection and the limit of quantification was 40 and 100 ng/zone, respectively. The average intra-day and inter-day precision was 0.7 and 2.2 %, respectively. Linearity was between 100-1300 ng/zone with a correlation coefficient of 0.999 and a %RSD of 2.3 %. Average recovery was 98.9 %.

extracts on polar-bonded stationary phases. J. of Chromatogr. A 1218 (19), 2812-2819 (2011). Two-dimensional TLC of phenolic compounds (extracted from Polygonum hydropiper L. and Polygonum cuspidatum L.) on cyano phase with non-aqueous solvents in the first direction and aqueous solvents in the second direction. For the separation of standards the optimal chromatographic systems was determined based on the retention data collected in one-dimensional TLC experiments by plotting graphs of Rf vs. Rf dependencies.

Der Pharmacia Letter 2(4), 440-446 (2010). TLC of glibenclamide on silica gel with toluene - ethyl acetate - methanol 16:1:2. The hRf value was 45. Quantitative determination by densitometry in absorbance mode at 229 nm. The method was linear in the range of 40-200 ng/band. The recovery was 99.8 %.

J. Liq. Chromatogr. Relat. Technol. 34, 920-927 (2011). TLC of flumequine in milk on silica gel with dichloromethane - methanol - 2-propanol - 25 % aqueous ammonia 3:3:5:2. Bioautography by dipping into an Escherichia coli bacteria suspension for 5 h at 37 ºC. After incubation, the plates were sprayed with 0.2 % MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide] aqueous solution and incubated for about 30 min at 37 ºC. The new procedure gave better recoveries of flumequine residues from milk compared with the previous method.

J. Liq. Chromatogr. Relat. Technol. 34, 2606-2620 (2011). HPTLC of dexketoprofen trometamol in pharmaceutical formulations on silica gel with toluene - ethyl acetate 3:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 255 nm. The hRf of 1 was 45. Linearity was 20-120 ng/zone. LOD and LOQ were found to be 5 and 10 ng/zone. Repeatability and intermediate precision (%RSD, n = 6) were below 2 %. Recovery (by standard addition) ranged between 98.8 and 99.3 %. The HPTLC method was suitable to determine the purity of the drug available from various sources by detecting the related impurities.

J. Trad. Chinese Veterinary Med. 5, 53-55 (2010). TLC of stachydrine on silica gel with acetone - ethanol - hydrochloric acid 10:6:1. Detection by spraying with bismuth potassium iodide - 1 % iron(III)chloride in ethanol 5:1 and heating at 100 ºC. Identification by comparison of the hRf value and zone color with the standard. Quantification of stachydrine by densitometry at 510 nm. Precision (%RSD within plate, n = 8) was 3.7 %. Stability of measurement (%RSD within 120 min, n = 5) was 4.5 %. Linearity was in the range of 3.2-38.3 µg/zone (r=0.997, n = 6). The recovery (by standard addition) was 96.6 % with a %RSD of 2.0 % (n = 6).

J. Planar Chromatogr. 24, 367-372 (2011). TLC of piroxicam, meloxicam, tenoxicam, and isoxicam on silica gel with ethyl acetate - ethanol - toluene 6:3:1 + 2 drops of 25 % ammonia. Quantitative determination by densitometry in absorbance mode at 360 nm. Linearity was between 50-500 ng/zone. The hRf value was 53 for piroxicam, 78 for meloxicam, 61 for tenoxicam, and 82 for isoxicam. LOD were 10, 30, 10, and 20 ng/zone and LOQ were 20, 80, 40, and 40 ng/zone for piroxicam, meloxicam, tenoxicam, and isoxicam, respectively. The recovery was in the range of 93.2-102.9 % with %RSD of less than 1 %. The inter- and intra-day precision (%RSD) was between 0.3-0.8 %.