Phytochem. Anal. 22, 258-262 (2011). TLC of gomisin A (1), gomisin N (2) and schisandrin (3) in the fruits of Schisandrae chinensis on silica gel with toluene - ethyl acetate - formic acid 14:6:1. Quantitative determination by direct analysis in real time mass spectrometry (DART-MS). Linearity of (1) - (3) was between 0.5 and 5 nmole. The limits of detection and quantification were 60-200 pmole for (1), and 58-192 pmole for (2) and (3). Recovery (by standard addition) for (1) - (3) was between 104.0 and 120.2 %. TLC-DART-MS method provides faster and more specific quantification compared with the conventional densitometric and HPLC-UV methods.

J. Chem. Pharm Res. 2(5), 92-96 (2010). TLC of ofloxacin and cefixime on silica gel with methanol - ethyl acetate - 25 % ammonia 7:7:3. The hRf value of ofloxacin was 61 and of cefixime 78. The method was linear in the range of 50-500 ng/band. The recovery was in the range of 99.3-102.2 %. Quantitative determination by densitometry in absorbance mode at 295 nm.

J. AOAC Int. 94, 735-742 (2011). HPTLC and TLC of ofloxacin on silica gel with ethanol - conc. ammonia 4:1 in a horizontal chamber. Quantitative determination by fluorescence measurement at 313 nm. Simulated samples at a residue level of 1 mg/m² were prepared by spreading the calculated amount of ofloxacin solution on 1, 5, and 10 dm² stainless steel surfaces. The hRf of ofloxacin was 56. The mean recovery (n = 6) was 88.6-95.3 % with a CV of 3.8-4.9 %. The LOD was 0.6 ng/zone and the LOQ was 2 ng/zone, but it was shown that these can be lowered by immersion of the developed plate into a solution of liquid paraffin - n-hexane 1:2 to approximately 0.3 and 0.9 ng/zone. The repeatability (system precision) was 4.2 % for 2 ng, and 3.7 % for 20 ng. The recovery was between 88.6-95.3 %.

J. AOAC Int. 93, 1836-1843 (2010). HPTLC of 1) lamivudine-zidovudine on silica gel with ethyl acetate - toluene - methanol 12:5:3, quantitative determination by absorbance measurement at 289 nm; of 2) metronidazole with ethyl acetate - ammonia 50:1, quantitative determination by absorbance measurement at 313 nm; of 3) neviparine with ethyl acetate - toluene 3:1, quantitative determination by absorbance measurement at 289 nm; and of 4) quinine with ethyl acetate - toluene - acetone 22:3:5, quantitative determination by absorbance measurement at 327 nm in a twin-trough chamber lined with wetted filter paper and saturated for 20 min. The average repeatability (within-laboratory) was 1.9 %, with 73 % less than 2 % and 97 % at 2.6 % or less. The average reproducibility (among-laboratory) was 2.7 %. Mean hRf values for lamivudine, metronidazole, neviparine, quinine, and zidovudine were 19, 28, 34, 33, 57.

J. Planar Chromatogr. 24, 57-59 (2011). HPTLC of piperine on silica gel with toluene - ethyl acetate - diethyl ether 6:3:1 in a saturated twin trough chamber. The hRf of piperine was 40. Quantitative determination by densitometry at 337 nm. Linearity was between 15 and 75 ng/zone. LOD and LOQ was 5 and 15 ng/zone, respectively. The recovery was 94.5 %. The instrumental precision, repeatability, intra-day and inter-day precision (%RSD, n = 6) was 0.6 %, 0.8 %, 0.9 and 0.8 %, respectively.

Chinese J. of Northwest Pharm. 26 (5), 324-327 (2011). TLC of the extracts of Kelu Oral Liquid on silica gel 1) for Ephedrae herba, with chloroform - methanol - concentrated ammonia 40:7:1, detection by spraying with 5 % ninhydrin in ethanol and heating at 105 °C, identification by fingerprint comparison with ephedrine/pseudoephedrine hydrochloride; 2) for Scutellariae radix, with ethyl acetate - butanone - formic acid - water 5:3:1:1, detection by spraying with 10 % FeCl3 in ethanol and heating at 105 °C, identification by fingerprint comparison with astragaloside IV; 3) for menthol and Tatarian Aster root, with petroleum ether (60-90 °C) - ethyl acetate 9:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, identification by fingerprint comparison menthol and shionone; 4) for Glycyrrhizae radix, with ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:2, detection under UV 365 nm after spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, identification by fingerprint comparison with glycyrrhizic acid ammonium salt; 5) for Loquat leaf, with cyclohexane - chloroform - ethyl acetate - glacial acetic acid 40:10:16:1, detection by spraying with 20 % phosphomolybdic acid in ethanol and heating at 105 °C, identification by fingerprint comparison with ursolic acid; 6) for Fritillaria cirrhosa D. Don, with ethyl acetate - methanol - concentrated ammonia 18:2:1, detection by spraying with 5 % potassium iodobismuthate and 0.2 % sodium nitrite solution, identification by fingerprint comparison with peiminine.

Research Journal of Pharmaceutical, Biological and Chemical Sciences 2(1), 6-11 (2011). HPTLC of tolterodine tartarate on silica gel with acetonitrile - water - formic acid 50:50:3 with chamber saturation for 15 min. Quantitative determination by densitometry in absorbance mode at 281 nm. The content of tolterodine tartarate in the formulation was calculated and found to be 99.1 %. The recovery (by standard addition) was between 99.1-100.1 %. LOD was 21 and LOQ 53 ng/zone. The intra-day and inter-day precisions (%RSD) were 0.05 and 0.08 %, respectively.

J. AOAC Int. 93, 787-791 (2010). HPTLC of omeprazole on silica gel with chloroform - methanol 9:1 in a twin-trough chamber after saturation for 20 min. Quantitative determination by absorbance measurement at 302 nm. The hRf value of omeprazole was 39. Linearity was between 50 and 3000 ng/band. The intra-day and inter-day precision was 0.4-0.5 and 0.8-0.9 % (n = 2). The recovery was 98.4-99.1 %. LOD and LOQ were 8 and 24 ng/zone, respectively.