59th Indian Pharmaceutical Congress C-100, 248, (2007). An HPTLC method has been developed for the estimation of flavonoids tannin and saponin in Caesalpinia bonduc. The dried powdered leaves of the plant were extracted with methanol and used for evaluation. HPTLC on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 20:2:2:5 for flavonoids, chloroform - glacial acetic acid - methanol - water 16:8:3:2 for saponin, and ethyl acetate - toluene - formic acid 6:6:1 for tannin. Detection by spraying with 5 % methanolic sulphuric acid for saponin, 5 % alcoholic aluminium chloride solution for flavonoids, and 5 % ferric chloride solution for tannin. Densitometric evaluation at 254 nm and 366 nm.

J. Sep. Sci. 30, 2053-2058 (2007). HPTLC of umbelliferone (1), psoralen (2), and eugenol (3) in the dried fruit pulp of Aegle marmelos and in the fruit of Trachyspermum ammi and Foeniculam vulgare on silica gel with toluene – methanol 19:1. Quantitative determination by absorbance measurement at 331 nm for (1), 304 nm for (2), and 280 nm for (3). The hRf values were 30, 58, and 70 for (1), (2), and (3), respectively. Linearity was between 1 and 5 ng/zone, 16 and 96 ng/zone, and 200 and 1000 ng/zone for (1), (2), and (3), respectively. The limits of detection and quantification were 0.8 and 1.2 ng/zone for (1), 8 and 16 ng/zone for (2), 60 and 150 ng/zone for (3), respectively. Recoveries were 98.9 %, 100.1 %, and 99.3 %, for (1), (2), and (3) respectively.

J. Planar Chromatogr. 20, 235-237 (2007). TLC of atorvastatin, cerivastatin, fluvastatin, lovastatin, and simvastatin on RP-18 in horizontal chamber with sandwich configuration with methanol - buffer eluents of different composition. The best selectivity - separation of all the compounds - was achieved with methanol - phosphate buffer pH 7.60 4:1. Detection and densitometry at 254 nm.

J. Sep. Sci. 30, 2086-2091 (2007). HPTLC of 4alpha-methyl-24beta-ethyl-5alpha-cholesta-14,25-dien-3beta-ol (1), 24beta-ethylcolesta-5,9,11,22E-trien-3beta-ol (2), and betulinic acid (3) in Clerodendrum inerme on silica gel with toluene - ethyl acetate 47:3. Quantitative determination by absorbance measurement at 620 nm. The hRf value was 48, 34 and 22 for compounds (1) , (2) and (3), respectively. Linearity was between 100 and 2500 ng/zone. The limits of detection and quantification were 5, 6, and 10 µg/mL and 14, 18, and 29 µg/mL, respectively, for (1), (2), and (3).

by TLC. Chromatographia 66 (7-8), 631-634 (2007). TLC of heraclenin and heraclenol in the roots of Heracleum candicans D.C. on silica gel with toluene - ethyl acetate 7:3. Quantitative determination by densitometry at 366 nmn. Linearity was between 4 - 10 µg/zone for heraclenin and 1 - 5 µg/zone for heraclenol.

59th Indian Pharmaceutical Congress F-24, 419, (2007). HPTLC of atenolol and losartan potassium on silica gel with ethyl acetate - methanol - 1,4 dioxane - ammonia 10:2:1:2 with chamber saturation for 10 minutes. Densitometry at 225 nm. Linearity was between 200 and 1000 ng/zone for both compounds. Recovery was between 97.9 and 99.5%.

J. Planar Chromatogr. 20, 303-306 (2007). TLC of p-hydroxybenzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, salicylic acid, butylated hydroxyanisol, and butylated hydroxytoluene on stannic silicate with n-hexane - ethyl methyl ketone - acetic acid 80:20:3. Quantitation by scanning densitometry at 270 nm. The limit of detection and quantitation for p-hydroxybenzoic acid was 0.05 and 0.51 µg/zone, respectively.

CBS 100, 6-7 (2008). HPTLC of the alkaloid deoxypeganine (from Peganum harmala) and bromination reaction products on silica gel over 60 mm with chloroform - methanol - acetone - cyclohexane 5:1:5:5 for Merck phases and 4:1:4:4 for Whatman phases. Quantitative determination by absorbance measurement at 254 nm.