62nd Indian Pharmaceutical Congress Abstract No. F-335 (2010). TLC of ampicillin and dicloxacillin on silica gel with n-butanol – water – formic acid 63:6:4. Quantitative determination by absorbance measurement at 220 nm. The hRf values of ampicillin and dicloxacillin were 85 and 69 respectively. The linearity was in the range of 1-6 µg/zone for both ampicillin and dicloxacillin. The recovery for ampicillin was 98.5-101.9 % and that for dicloxacillin was 98.3-101.3 %.

62nd Indian Pharmaceutical Congress Abstract No. F-237 (2010). TLC of tolterodine tartarate on silica gel with acetonitrile – water – formic acid 50:50:3. Quantitative determination by absorbance measurement at 281 nm. The method was found to be linear in the range of 1-30 µg/band.

62nd Indian Pharmaceutical Congress Abstract No. F-242 (2010). TLC of gatifloxacin and ornidazole on silica gel with n-butanol – ethanol – 8M ammonia 10:1:3. Quantitative determination by absorbance measurement at 299 nm. The hRf value of gatifloxacin was 27 and of ornidazole 83. The method was found to be linear between 20-100 ng/zone for gatifloxacin and 50-250 ng/zone for ornidazole (r2> 0.99). The limit of detection and quantitation were found to be 4.1 and 12.5 ng/zone, respectively for gatifloxacin and 10.3 and 31.2 ng/zone, respectively for ornidazole.

Acta Chromatographica 21(2), 299-317 (2009). HPTLC of amtolmetin guacil on silica gel with toluene – ethyl acetate 2:3. Identification and quantification by densitometric analysis in absorbance mode at 320 nm. The samples were subjected to acidic and alkaline hydrolysis, oxidation, dry heat treatment, and photo-degradation. The method was suitable for stability studies and to study the kinetics of degradation of amtolmetin guacil.

J. Chinese Modern Med. & Pharm. 17 (34), 52-53 (2010). TLC on silica gel with chloroform – ethyl acetate – methanol – formic acid 2000:25:50:1. Detection under UV 366 nm. Identification of rhaponitin in both medicines by comparison with the standard.

J. Strait Pharm. 22(11), 90-92 (2010). TLC of components of Yinhu granules: 1) for Artemisia capillaris Thunb. on polyamide phase with acetic acid; 2) for Radix Notoginseng on silica gel with chloroform – methanol – water 13:7:2. Detection 1) under UV 366 nm; 2) by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones were visualized.

J. Planar Chromatogr. 24, 274-280 (2011). Presentation of new reagents and visualization methods which enable the detection of particular substances. The best of them provide high selectivity and low detection limits and lead to a reliable analysis of the developed chromatogram. Three groups of chemical reactions are used for detection: oxidation, reduction, and complexation, as well as precipitation of colored precipitates. Oxidation and reduction are most widely used due to their selectivity, which allows detection and identification of the separated substances. Like this the target substance can be selectively visualized, as for example antioxidants in complex natural matrices. Innumerable chemical, physical, and biological methods of visualization are known. Required are new, more efficient reagents fo a selective visualization of a defined part of a substance or, in some cases, for a specific determination of a specific substance. The article describes the detection of food ingredients, medicines and pharmaceuticals, organic compounds not present in food, and inorganic compounds.

J. Liq. Chromatogr. Relat. Technol. 34, 902-919 (2011). HPTLC of seven sugars (D-glucose, D-galactose, D-mannose, beta-D-fructose, alpha-D-fructose, sucrose, maltose, lactose) in food samples on silica gel with n-butanol - isopropanol - acetic acid - boric acid solution (200 mg boric acid in 10 mL water) 6:14:1:3. Detection by dipping into either aniline diphenylamine o-phosphoric acid reagent or p-aminobenzoic acid reagent. Quantitative determination by absorbance measurement at 370 nm. The HPTLC method was more sensitive by a factor of 8 for detection of sugars when compared to HPLC; only fructose showed a slightly better LOQ (difference by factor of 3). LOQ was better than 63 ng for HPTLC and about 500 ng for HPLC. Method comparison showed a good correlation and only a mean difference between both methods of 1.5 % sugar content for many food samples analyzed. HPTLC is a fully compliant method for determination of sugars in food. Application in the bioanalytical field was shown as well.