J. Sep. Sci. 31, 71-77 (2008). HPTLC of acrylamide in drinking water on silica gel, derivatization in situ with 5-dimethylamino-naphtalene-1-sulfinic acid (1.6 µg/µL in methanol), followed by heating at 120 °C for 1 hour and developed with ethyl acetate. For fluorescence enhacement, the plate was dipped into a solution of 25 % polypropylene glycol in n-hexane and dried immediately. Quantitative determination by fluorescence at 366/>400 nm. Verification was based on HPTLC-ESI/MS, HPTLC-direct analysis in real time (DART)-TOF/MS and NMR. The hRf value of acrylamide (as 3-dansylpropanamide) was 69. Linearity was between 0.1 and 0.4 µg/L. Within-run precision and the mean between-run precision (n=3) were 4.6 and 11.0 %. The limit of detection and quantification for acrylamide was 0.025 and 0.083 µg/L, respectively. Recovery (by standard addition) was 96.4 %. The method showed comparable result with HPLC-MS/MS.

Indian Drugs 45(4), 301-306 (2008). HPTLC of diclofenac (extracted with 3N HCl and chloroform from single and multi-component diclofenac gel formulations) on silica gel with toluene - ethyl acetate - acetic acid 600:400:2. The hRf value of diclofenac was 39, of salicylic acid 29, and of methyl salicylate 83. Quantitative determination by absorbance measurement at 283 nm. Linearity was between 200 and 600 ng/spot via peak area. In single component gel, recovery was 100.4 % whereas in multi-component gel it was 99.5 %. The method was found to be accurate and suitable for analysis in single and multi-component gel formulations.

Asian J. Chem. 20(4), 2531-2538 (2008). TLC of melatonin on silica gel with toluene - ethyl acetate - formic acid 10:9:1. Quantitative determination by absorbance measurement at 290 nm. The method was linear in the concentration range of 100 to 600 ng/spot. The recovery of the drug from tablets (by standard addition method) was 99.7 %. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of the drug. Forced degradation studies showed the effect of variations in pH, UV light and high temperature on the stability of melatonin. As the proposed method could effectively separate the drug from its degradation products, it can be employed as a stability indicating method.

Indian Drugs 45(3), 222-225 (2008). HPTLC of atisine in Aconitum heterophyllum (Ranunculaceae) on silica gel with toluene - ethyl acetate - diethylamine 7:2:1. Absorbance measurement at 274 nm prior to derivatization. Detection by dragendorff’s reagent followed by treatment with 10 % sodium nitrite. Quantitative determination by absorbance measurement at 520 nm. Linearity was between 10-60 ng/spot.

60th Indian Pharmaceutical Congress PA-205, (2008). HPTLC of diacerein on silica gel with ethyl acetate - n-hexane - acetic acid 120:19:1. The hRf value was 32. Quantitative determination by absorbance measurement at 258 nm. The method was linear in the range of 50-250 ng/spot. The recovery was 96-103 %.

J. AOAC Int. 91, 1174-1178 (2008). HPTLC of hederagenin on silica gel with toluene - ethyl acetate - formic acid 7:3:5 in a twin-trough chamber. Detection with anisaldehyde - sulfuric acid reagent by dipping for 1 s and heating for 7 min at 100 °C. Quantitative determination by absorbance measurement at 595 nm.

J. Liq. Chromatogr. Relat. Technol. 31, 1913-1924 (2008). TLC of eleven anti-depressive drugs (amitriptyline, doxepin, amizepin, chlorpromazine, clomipramine, flupentixol, haloperidole, moclobemide, perazine, risperidone, venlafaxine) on silica gel, RP-18 and cyano phased in a horizontal chamber with non-aqueous mobile phases containing of polar modifier - methanol, medium polar diluent - diisopropyl ether and aqueous ammonia or diethylamine. The best results were obtained with addition of ammonia. Detection under UV light and by videodensitometry.

60th Indian Phamaceutical Congress PA-196, (2008). HPTLC of trandolapril on silica gel with chloroform - methanol - acetic acid 16:3:1 in a saturated chamber. Quantitative determination by absorbance measurement at 210 nm. Linearity was in the range of 200-1000 ng/spot. The method was suitable for routine quality control of drug in tablet dosage form.