J. Planar Chromatogr. 18, 39-43 (2005). Micropreparative TLC of 10 pesticides (isoproturon, diuron, momolinuron, desmetryn, methiocarb, atrazine, fenitrothion, terbutryn, bromopropylate, aziprotryne) for preliminary fractionation on silica gel in horizontal DS chambers with tetrahydrofuran - n-heptane 1:4; detection under UV at 254 nm. HPTLC of the separated fractions on RP-18 W with methanol - water 3:2 and acetonitrile - water 3:2; densitometry at 254 nm.

J. Planar Chromatogr. 18, 290-293 (2005). TLC of gallic acid in tea extracts (gallic acid, caffeine, (+)-catechin, and tannic acid as standards) on silica gel in an unsaturated chamber with chloroform - ethyl acetate - formic acid 5:4:1. Densitometric measurement at 289 nm. Limit of detection 0.1 µg per spot.

J. AOAC Int. 88, 70-79 (2005). HPTLC of haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in 25 selected psychotropic drugs on silica gel with 30 mobile phases, eight of them were selected based on spot location and developing time. Identification and quantification were carried out based on UV densitometric measurements. In addition to retention factors, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Limit of detection ranged from 0.009 to 0.260 µg depending on the wavelength used. A satisfying recovery, ranging from 92.9 to 104.7 %, was achieved for individual constituents.

J. Pharm. Biomed. Anal. 39, 801-804 (2005). HPTLC on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The hRf values of stavudine (SV), lamivudine (LV) and nevirapine (NV) were 25, 67 and 87 respectively. The method was linear within the range of 0.01-0.06 mg/mL, 0.05-0.30 mg/mL and 0.06-0.40 mg/mL for SV, LV, and NV respectively, recovery rates were between 98.2 and 99.9 %. The HPTLC method was compared with the UV and HPLC methods. Accuracy, precision and ruggedness of the method were established.

J. Planar Chromatogr. 18, 294-299 (2005). TLC of (+/-)-metoprolol tartrate and (-)-alprenolol tartrate on silica gel, with concentrating zone, on RP-18, on LiChrospher silica gel and on alumina, previously impregnated with the mobile phase (ethanol - water 7:3, containing D-(-)-tartaric acid as chiral selector). Detection under UV light at 254 nm and densitometric scanning at 230 nm. Direct separation of the enantiomers of (+/-)-metoprolol tartrate was achieved.

J. Planar Chromatogr. 19, 139-145 (2006). Analytical OPLC-TLC and preparative TLC of ascorbigen and 1’-methylascorbigen on silica gel with chloroform - methanol 9:1. Visualization by use of Procházka’s reagent (reaction with formaldehyde) as derivatization agent. Visual evaluation at 366 nm and by densitometry at 440 nm.

Abstract GP-41, IPC (2005). HPTLC of tizanidine in tablets on silica gel with ethyl acetate - methanol - acetic acid 60:4:1. Linearity range was 0.5-0.6 µg, LOQ 0.5 µg, and average recovery was 99.4-101.6 %. The method was validated according to ICH guidelines.

J. Pharm. Biomed. Anal 37 (5), 971-977 (2005). HPTLC of colchicine in Colchicum autumnale (meadow saffron) extracts, on silica gel with chloroform - acetone - diethyl amine 5:4:1. Quantification in absorbance mode at 350 nm. The peaks were optimized in area and shape by varying 4 scanning parameters (slit width and height, number of measurements and scanning speed). Calibration range of pure colchicine was 50-600 ng/mL. This method can be used in pharmaceutical industry for quick and accurate quantitative determination of colchicine because it eliminates the inteferences given by other bioactive or degradation compounds. The method was validated regarding linearity, accuracy, fidelity, and sensitivity.