J. Planar Chromatogr. 28, 36-41 (2015). HPTLC of (1) gallic acid and (2) kaempferol during the processing of the Ayurvedic formulation Arista (herbal material fermented with flowers of Woodfordia fruticosa) on silica gel with toluene – ethyl acetate – formic acid – methanol 15:15:4:1. The hRF values of (1) and (2) were 50 and 58, respectively. _x000D_

Beijing J. Trad. Chinese Med. 32 (6), 457-459 (2013). Qingfei Wan pill is a traditional Chinese compound herbal medicine prescribed for the treatment of fever, cough, runny nose, sore throat, etc. For quality control, TLC on silica gel (1) for Gardenia jasminoides Ellis and gardenoside with chloroform – methanol – ethyl acetate – ammonia 20:8:4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating until the zones are visible; (2) for Belamcanda chinensis L. Redouté with chloroform – methanol – ammonia 40:10:1, detection at UV 254 nm; (3) for Terminalia chebula Retz. with chloroform – ethyl acetate – formic acid 6:4:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation under white light.

J. Planar Chromatogr. 28, 223-228 (2015). HPTLC of beta-amyrin in the leaves of five different Ficus species (F. carica, F. nitida, F. ingens, F. palmata and F. vasta) on silica gel with toluene - methanol 9:1. Detection with p-anisaldehyde reagent followed by heating. Quantitative determination by absorbance measurement at 550 nm. Precisions (intra- and inter-day) were 1.6-1.8 % and 1.7-1.8 % respectively (n=6). The hRF value was 58. Linearity was between 100 and 900 ng/zone. The LOD and LOQ were 32 ng and 96 ng/zone, respectively. Recoveries were 97.6-98.3 %.

Phytochem. Anal. 27, 5-12 (2015). TLC-bioautography (editors note: TLC-autography) of lipase inhibitors in the stems of Flammulina velutiper and Pleurotus eryngii and the roots of Panax quinquefolius on silica gel with chloroform - ethyl acetate - methanol - water 15:40:22:9. The plates were dipped in β-naphthyl myristate solution, followed by air-drying at room temperature and dipping into lipase solution (60 U/mL), followed by incubation at 37 °C for 20 min. After dipping in 1 mg/mL of Fast Blue B salt solution, the plates were incubated again. Detection at 606 nm. Relative lipase inhibitory activity was calculated as orlistat equivalents. LOD was 0.01 ng/zone for orlistat. The intermediate precision was below 4.9 % (n=3). Recovery was in the range of 92-105 %.

J. Food Compos. Anal. 48, 25-33 (2016). In this study 353 samples of different varieties and different commercial products were analyzed by HPTLC and UV absorbance. HPTLC of the acetonic extract of kava powder on silica gel with hexane – dioxane 4:1 without chamber saturation over a distance of 85 mm. Detection under UV 254 nm and 366 nm. Densitometric evaluation and determination of the ratios kavain/total kavalactones (K/KL) at 254 nm and flavokavins/kavalactones (FK/KL) at 366 nm. Derivatization by dipping in anisaldehyde sulfuric acid reagent (10 mL sulfuric acid, 170 mL methanol, 20 mL acetic acid, 1 mL anisaldehyde) and heating at 100 °C for 3 min. The results showed that noble varieties suitable for daily consumption of kava are characterized by high K/KL and low FK/KL.

Planta Medica 82 (17), 1475-1481 (2016). Four new flavone glycosides (acacetin group) isolated from Piper aduncum leaves were submitted to acidic hydrolysis; TLC of the aqueous fractions, purified with ethyl acetate, on silica gel and RP-18 with chloroform – methanol – water 30:20:3. Detection by spraying with sulfuric acid reagent, followed by heating. By comparison to standards (applied in overlay and in distinct tracks), the constituents of the sugar moieties could be identified as apiose, glucose and rhamnose.

Planta Medica 83(01/02), 143-150 (2017). Preparative TLC on silica gel H to purify amide alkaloids from column chromatography apolar subfractions of aerial part methanolic extracts of: a) Piper flaviflorum: decenoylpiperidide (petroleum ether – acetone 10:1), N-methoxycinnamoyl-pyrrolidine, dihydropiperyline, stigmalactam, chingchengenamide A (petroleum ether – ethyl acetate 8:1); and b) P. sarmentosum: methylenedioxyphenyl-undecadienoyl/undecatrienoyl-pyrrolidine (petroleum ether – acetone 1:2), cinnamoyl-hydroxypyrrolidine and deacetylsarmentamide B (chloroform – methanol 9:1); detection with Dragendorffʼs reagent.

J. Chromatogr. A 1530, 192-196 (2017). HPTLC to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea, which can be a traditional medicine as alternative treatment for diabetes, because the substances produced by ants can reduce blood sugar levels. Measurement of the antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts with the HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH*) assay, and of the hypoglycemic effects with a newly developed α-amylase inhibitory activity assay. Detection of stigmasterol after derivatization with anisaldehyde reagent as purple colored zone under white light at hRF 66. The results showed the highest antioxidant activity in the ethanol extract rich in polyphenols and flavonoids. No antioxidant activity but significant α-amylase inhibitory activity was observed in the DCM extract. The highest α-amylase inhibitory activity was found in the EA and DCM extracts and was related to their stigmasterol content.