J. Liquid Chromatogr. 15, 337-349 (1992). TLC of urinary tryptamide metabolites before and after beta-glucuronidase treatment on silica with 1) chloroform - methanol - acetic acid 70:20:5, 2) chloroform - methanol - acetic acid 32:4:1. Detection by spraying with Ehrlich reagent containing 250 mg p-trimethylaminobenzaldehyde in 100 mL of a mixture of ethanol and conc. hydrochloric acid 1:1.

3rd Communication: Identification of metabolites in rat urine. Arzneim.-Forsch./Drug Res. 46, 127-133 (1996). TLC of montirelin hydrate and 3 metabolites (deamidation and deprolination products) on silica with ethanol - 0.1 N hydrochloric acid 10:3 and chloroform - ethanol - 28% NH3. Detection by autoradiography, visualization of authentic compounds and nonradioactive metabolites under UV 240 nm.

JAOAC Int. 84, 1265-1276 (2001). Within the process of the International Conference on Harmonization (ICH), 2 guidelines were released containing a standardized terminology, a verified model of requirements for the validation of analytical procedures, and some guidance in the practical aspects of conducting validation studies in pharmaceutical analysis. For planar chromatographic procedures, which may be used at different levels either in qualitative identity testing, assays, semiquantitative limit tests, or quantitative determination of impurities, it was tried to transfer these formal requirements into practical approaches for validation. Topics like prevalidation considerations and validation (identification, testing for impurities with the limit test, testing for impurities by using quantitative procedures, robustness, quality assurance in routine work) were discussed.

59th Indian Pharmaceutical congress C- 260, 287, (2007). HPTLC of artemisinin, quercitin and rutin from Artemisia dracunculus (Asteraceae) on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - water 100:25:10:10:1 for artemisinin, and n-hexane - acetone - ethyl acetate 16:1:1 for quercitin and rutin. Evaluation under UV 254 nm. The hRf values were 15, 26 and 91 for quercitin, artemisinin, and rutin, respectively. The alcoholic extract (dried) of the plant was found to contain 0.84 % artemisinin, 0.14 % quercitin, and 0.019 % rutin.

J. Chromatogr. Sci. 46 (1), 10-14 (2008). Quantitative determination of rabeprazole and mosapride in their combined pharmaceutical dosage forms by TLC on silica gel with ethyl acetate - methanol - benzene 4:1:5. Quantification by densitometry at 276 nm. Linearity was between 400 and 1200 ng/spot for rabeprazole and between 300 and 900 ng/spot for mosaprid, respectively. The limit of detection was 132 ng/spot for rabeprazole and 98 ng/spot for mosapride.

Anal. Chim. Acta, 576 (2), 253-260 (2006). Description of a simple, sensitive, selective, precise and stability indicating method for determination of gatifloxacin both as a bulk drug and from polymeric nanoparticles by TLC on silica gel with n-propanol - methanol - 25 % ammonia 50:10:9. The hRf value of gatifloxacin was 60. Separation from degradation products (produced under acidic and basic conditions and upon wet and dry heat treatment) was good. Quantitative determination by absorbance measurement at 292 nm. Linearity was 400 - 1200 ng/spot, with a correlation coefficient of 0.9953. The limit of detection and quantitation was 3 and 8 ng/spot, respectively.

IPA Convention, 2010, RA-PO 03. HPTLC of edaravone in samples of human plasma (purified by liquid-liquid extraction) on RP-18 with n-butanol - methanol - diethyl ether 1:8:1. The compound was well resolved with an hRf value of 81. Densitometric evaluation at 240 nm. The limit of detection and quantification was 25 ng and 150 ng respectively. The linearity was 600-2400 ng/band. Recovery (%) based on analysis of spiked sample was more than 65 %.

Acta Chromatographica 20(4), 709-719 (2008). HPTLC of trigonelline and 4-hydroxyisoleucine from Fenugreek seeds (Trigonella foenum-graceum) on silica gel with n–butanol – methanol – acetic acid – water 8:3:2:2. Detection by spraying with ninhydrin reagent. Quantification by densitometry at 266 nm for trigonelline, and at 395 nm for 4-hydroxyisoleucine. The linearity was in the range of 100-1000 ng/band for trigonelline and 50-500 ng/band for 4-hydroxyisoleucine, respectively, with r=0.9992 and 0.9986 respectively. The average recovery at three different levels was 99.4 % for trigonelline and 99.1 % for 4-hydroxyisoleucine.