Planta Medica 83(01/02), 143-150 (2017). Preparative TLC on silica gel H to purify amide alkaloids from column chromatography apolar subfractions of aerial part methanolic extracts of: a) Piper flaviflorum: decenoylpiperidide (petroleum ether – acetone 10:1), N-methoxycinnamoyl-pyrrolidine, dihydropiperyline, stigmalactam, chingchengenamide A (petroleum ether – ethyl acetate 8:1); and b) P. sarmentosum: methylenedioxyphenyl-undecadienoyl/undecatrienoyl-pyrrolidine (petroleum ether – acetone 1:2), cinnamoyl-hydroxypyrrolidine and deacetylsarmentamide B (chloroform – methanol 9:1); detection with Dragendorffʼs reagent.

J. Chromatogr. A 1530, 192-196 (2017). HPTLC to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea, which can be a traditional medicine as alternative treatment for diabetes, because the substances produced by ants can reduce blood sugar levels. Measurement of the antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts with the HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH*) assay, and of the hypoglycemic effects with a newly developed α-amylase inhibitory activity assay. Detection of stigmasterol after derivatization with anisaldehyde reagent as purple colored zone under white light at hRF 66. The results showed the highest antioxidant activity in the ethanol extract rich in polyphenols and flavonoids. No antioxidant activity but significant α-amylase inhibitory activity was observed in the DCM extract. The highest α-amylase inhibitory activity was found in the EA and DCM extracts and was related to their stigmasterol content.

Phytochemistry 155, 37-44 (2018). HPTLC of pine resins collected from Abies grandis, Pseudotsuga menziesii, Picea abies, Pinus sylvestris L. and Pinus strobus L. in a forest area of The Netherlands during early and late spring on silica gel with toluene – isopropyl alcohol – diethyl ether 16:1:3. Detection by spraying with 2 mL of anisaldehyde sulfuric acid solution, followed by heating at 100 ºC for 3 min. Qualitative identification under UV light at 366 nm. Most of the metabolites detected in GC-MS were confirmed by HPTLC analysis. HPTLC proved to be an important technique for metabolic profiling.

Chem. Pharm. Bull. 32, 4942-4945 (1984). TLC of 7 mutagenic xanthones on silica. Quantification by densitometry. Investigation of the conditions of the analysis. The relationship between xanthone content and mutagenicity is discussed.

aurea. Phytochemistry 25, 1443-1447 (1986). TLC of complex mixtures of quinolizidine alkaloids on silica with chloroform - methanol -28 % NH3 90:9:1, isopropanol - ethyl acetate - chloroform -28 % NH3 11:4:4:1, diethylamine - chloroform 1:9 or ether - methanol -28 % NH3 44:5:1. Detection with Dragendorff reagent. Isolation of the alkaloids (calpaurine and related compounds) from crude extracts by PLC.

TLC of berberine on silica with butanol -36 % formic acid - water 2:1:1. Determination by densitometry at 400 nm.

Discussion of factors such as sample size, pH values of mobile and stationary phases, layer quality, conditions of development.

(Hungarian). Acta Pharm. Hungarica 57, 209-217 (1987). TLC of absinthin, foliamenthin, genciopicrozide, naringin, neohesperidin on silica with acetone - chloroform - water 80:20:5 or 145:40:15. Detection after spraying with 1% vanillin solution or 5% H2SO4 methanolic solution under UV 254 and 366 nm. Densitometry.