J. Planar Chromatogr. 10, 348-352 (1997). Development of a simple, rapid and reliable TLC method for the identification and quantification of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in urine. HPTLC on silica gel 60 WRF254s layers (0.1 mm layer thickness) with dichloromethane - hexane - methanol 7:2:1. Because the chromatograms were evaluated directly by HPTLC-UV and HPTLC-FTIR on-line coupling, it was not necessary to derivatize with fast blue salts or other health-endangering azo dyestuffs. The validated detection limits for THC-COOH (UV: 4 ng/mL, IR: 14 ng/mL) enabled qualitative and quantitative evaluation in the region of the 20 ng/mL cutoff.

Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 321-326. TLC/AMD and OPLC (Optimum Performance Laminar Chromatography) of Opium extract and morphine , codeine, thebaine, papaverine, noscapine, ethylmorphine, pholcodine, heroin, buprenorphine, dextromethorphan, methadone as reference substances: TLC of Opium alkaloids on silica gel with toluene - acetone - ethanol - 28% NH3 45:45:7:1. OPLC on aluminium oxide with ethyl acetate as mobile phase and AMD with methanol, acetone, ethyl acetate, ethyl acetate/dichloromethane, dichloromethane. TLC is quick, simple, cheap, detection is easy and permits densitometry; OPLC is a fast method; AMD presents the best resolution, no diffusion of spots, elution without oxidation and is considered as a very interesting method for densitometry.

J. Sep. Sci. 30, 2053-2058 (2007). HPTLC of umbelliferone (1), psoralen (2), and eugenol (3) in the dried fruit pulp of Aegle marmelos and in the fruit of Trachyspermum ammi and Foeniculam vulgare on silica gel with toluene – methanol 19:1. Quantitative determination by absorbance measurement at 331 nm for (1), 304 nm for (2), and 280 nm for (3). The hRf values were 30, 58, and 70 for (1), (2), and (3), respectively. Linearity was between 1 and 5 ng/zone, 16 and 96 ng/zone, and 200 and 1000 ng/zone for (1), (2), and (3), respectively. The limits of detection and quantification were 0.8 and 1.2 ng/zone for (1), 8 and 16 ng/zone for (2), 60 and 150 ng/zone for (3), respectively. Recoveries were 98.9 %, 100.1 %, and 99.3 %, for (1), (2), and (3) respectively.

Indian Drugs 44(8), 636 (2007). HPTLC of charantin in chloroform extracts of herbal formulations on silica gel with benzene - methanol 4:1. Detection by spraying with 10 % alcoholic sulphuric acid and heating at 130 °C for 2 min. Quantitative determination by densitometry at 536 nm. The hRf of charantin was 32. Linearity was between 20 and 600 ng/mL. Recovery was 98.9 %

59th Indian Pharmaceutical congress F-217, 442, (2007). HPTLC of chloroform, ethanol and water extracts of Nothapodytes foetida on silica gel with toluene - ethyl acetate - glacial acetic acid 25:5:1. Densitometry at 366 nm. The hRf value of camptothecin was 22. Linearity was between 0.5 and 5 ng/zone. All commercial samples of the plant had a similar fingerprint profile.

Food Chem. 108, 1039-1053 (2008). HPTLC of luteolin-7-O-beta-glucuronide (1), lithospermic acid (2), and rosmarinic acid (3) in Lamiaceous species (Thymi herba, Serpylli herba, and Majoranae herba) on amino phase for (1) and silica gel for (1), (2), and (3) with acetone - formic acid 17:3 and diisopropyl ether - acetone - formic acid - water 5:3:1:1, respectively. Evaluation at 365 nm before and after spraying with 2 % methanolic aluminium chloride, natural product/polyethylene glycol reagent or in visible light after treatment with bis-diazotized sulfanilamide (yellow, orange, red, and brown bands).

Pharmazie 63, 389-393 (2008). TLC of methyl brevifolincarboxylate and kaempferol 3-O-beta-D-(6’’-E-p-coumaroyl)-glucopyranoside tiliroside on silica gel with ethyl acetate - methanol - water 18:1:1. Detection under UV light.

Anal. Chim. Acta 577 (1), 46-51 (2006). HPTLC of trigonelline in herbal extracts and in pharmaceutical dosage forms on silica gel with n-propanol - methanol - water 4:1:4. Quantitative determination by absorbance measurement at 269 nm. Linearity was between 100 - 1200 ng/spot (via peak area) and the correlation coefficient was 0.9991. The trigonelline content of herbal extracts quantified and estimated from the formulation was found to be well within limits (± 5 %) of the labeled content of the formulations. The method is reproducible and selective for the estimation of trigonelline by statistical analysis of the data.