Abstract No. F-299, 61st IPC (2009). HPTLC of herbal powder and polyherbal formulation containing Andrographis paniculata on silica gel with benzene - ethyl acetate 1:1. Quantitative determination by absorbance measurement at 222 nm. The calibration curve for andrographolide was linear in the range of 360-660 ng/band.

J. Ethnopharmacol. 123, 244-249 (2009). HPTLC of kaempferol-3-O-rutinoside and astragalin in leaves of Gynura procumbens on silica gel with acetic acid – methanol – dichloromethane 1:3:7. Quantitative determination by absorbance measurement at 366 nm. The hRf values of kaempferol-3-O-rutinoside and astragalin were 43 and 72, respectively, and selectivity regarding matrix was given. Linearity was given between 16 and 1000 µg/mL and the correlation coefficients were >0.987.

60th Indian Pharmaceutical Congress PG-261 (2008). HPTLC of quercetin and rutin in ethanolic extracts of aerial parts of Tylophora indica and Tephrosia purpurea on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:26. Quantitative determination by fluorescence measurement at 366/>400 nm. The extract of Tephrosia purpurea contained 1.56 % of quercetin and 1.40 % of rutin, whereas Tylophra indica contained 4.30 % of quercetin.

Abstract No. 9933, IHCB (2009). HPTLC of colchicines in Gloriosa superba (collected from different parts of India) on silica gel with ethyl acetate - methanol 200:27. The hRf value of colchicine was 29. Quantitative determination by absorbance measurement at 350 nm. The method was linear in the range of 50-1000 ng/spot. The sample collected from Kerala was found to contain highest level of colchicines (0.24 %).

Abstract No. C-338, 61 IPC (2009). HPTLC of gallic acid and piperine in combined formulation on silica gel with toluene - ethyl acetate - formic acid 16:8:1. Quantitative absorbance measurement at 320 nm. The method was linear in the range of 200-800 ng/band for gallic acid and 50-350 ng/band for piperine.

J. Planar Chromatogr. 23, 180-183 (2010). TLC of chamomile extracts on silica gel with benzene - ethyl acetate 19:1 or chloroform - methanol - water 40:10:1 in an unsaturated chamber. Detection at 254 and 366 nm. For bioautographic evaluation bioluminescent Bacillus subtilis or Pseudomonas syringae pv. maculicola were used; visualization by dye a reagent was achieved by dipping the plate in an aqueous solution of MTT. Quantitative determination by densitometric scanning at 300 nm (before biological detection) or at 590 nm (after visualization of the bioautogram with MTT).

Abstract No. C-454, 61st IPC (2009). HPTLC of ursolic acid and luteolin from aerial parts of Lippia nodiflora on silica gel with toluene - ethyl acetate - formic acid 70:30:3. The hRf value of luteolin was 34 and of ursolic acid 85. Densitometric analysis at 254 nm for luteolin and at 530 nm for ursolic acid after derivatization with natural products reagent followed by PEG reagent. The recovery of both marker components was in the range of 98.6-100.5 %.

The Open Nutraceuticals Journal 2, 2-6 (2009) A TLC method using a micellar solution of sodium dodecyl sulfate (SDS) as mobile phase has been developed for identification of four herbals present in Jatiphaladya, a powdered herbal formulation containing Cannabis sativa, Myristica fragrans, Piper longum, and Embleia ribes. The formulation was extracted with 80 % ethanol. TLC on laboratory made plates coated with silica gel and activated at 100 °C for 60 min, with a 5 % solution of SDS as mobile phase. The resolved spots were identified by spraying with a 2 % solution of vanillin in 5 % methanolic sulfuric acid. Spots corresponding to different herbals were well resolved. Different detection reagents were evaluated, i.e. iodine, vanillin sulfuric acid, and anisaldehyde-sulfuric acid. Vanillin sulfuric acid reagent was found to be the most sensitive. Of the different surfactants used, anionic, cationic and nonionic, SDS was found to be most suitable. The most suitable pH of the mobile phase was pH 4.2-5.7, it provided optimum resolution of zones.