J. Planar Chromatogr. 24, 306-311 (2011). HPTLC of ethanolic leaf extracts of A. squamosa, with rutin and isoquercitrin as standards, on silica gel (prewashed with methanol) with ethyl acetate - formic acid - glacial acetic acid - ethyl methyl ketone - water 50:7:3:30:10 with chamber saturation for 30 min at room temperature (25 +/- 2°C) and a relative humidity of 50 +/- 5 %. Quantitative determination by densitometry at 366 nm. Linearity was between 200-1600 ng/band for both rutin and isoquercitrin. The robustness of the method (%RSD) was 1.9-2.9 % for rutin and 1.5-2.3 % for isoquercitrin, respectively. The instrumental precision (%RSD) was 0.9 and 0.3 % for rutin and isoquercitrin, respectively. The intra-day and inter-day precision (%RSD) was less than 3 % in all cases. LOD and LOQ was 75 and 100 ng/band for rutin and 40 and 80 ng/band for isoquercitrin, respectively. The hRf value was 32 for rutin and 58 for isoquercitrin. Recovery (by standard addition) was found to be 96-107 %.

PHCOGJ 2(8), 211-215 (2011). TLC finger-print profiling of 4 cinnamomum species, C. malabatrum, C. sulphuratum, C. tamala and C. zeylanicum has been reported. The hexane extracts of the plants were separated on silica gel with toluene - ethyl acetate 8:1. Densitometric evaluation at 254 nm. Detection by dipping in vanillin-sulfuric acid reagent followed by heating at 105 °C and evaluation at 620 nm. The hRf value of eugenol was 69. The content of eugenol were higher in C. zeylanicum than in the other species. The fingerprint profile showed some other bands.

J. of China Pharm. 21 (20), 34-35 (2012). Zhongtongxiao capsules are a herbal TCM preparation to treat blood stasis and sore swellings caused by fracture. TLC of the extracts of the medicine on silica gel 1) for Angelica sinensis and the rhizome of Chuanxiong, with n-hexane – ethyl acetate 9:1, detection at UV 366 nm; 2) for Radix Paeoniae, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and heating at 105 °C until the zones are visible, viewing in daylight; 3) for Teasel, developed with toluene – ethanol 9:2, detection by spraying firstly with potassium iodobismuthate – hydrochloric acid – water 10:1:200 and then with 5 % nitrous acid in ethanol, followed by viewing in daylight; 4) for Licorice, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, viewing in daylight.

J. of Chinese Med. 27 (166), 344-345 (2012). Biqiang Bidou flushing fluid is a herbal TCM preparation for treatment of forehead headaches and sinusitis. For quality control, TLC on silica gel 1) for Honeysuckle and the standard chlorogenic acid, with ethyl acetate – formic acid – water 15:12:5, detection under UV 366 nm; 2) for Baical Skullcap, with ethyl acetate – acetone – acetic acid – water 10:4:5:3, detection by spaying with 5 % ferric chloride in ethanol and viewing in daylight.

by thin-layer chromatography) (Chinese) Chinese J. Ethnomed. Ethnopharm.(3), 53-54 (2012) Melodinus fusiformis is a herbal TCM drug specially effective for curing rheumatism. TLC of the extractsand standard oleanolic acid of the crude drug on silica gel with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % phosphomolybdate in n-propanol and heating using a air blower until the zones are visible.

J. of China Pharm. 21 (3), 16-18 (2012). Dingxuan granules are a herbal TCM preparation with nourishing effect on liver and kidney and curative effect on Meniere's syndrome. For quality control, TLC of the extracts of the medicine 1) for Radix Polygoni multiflori preparata, on silica gel first over 3.5 cm with chloroform – methanol 7:3, then over 7 cm with chloroform – methanol 20:1, detection at UV 366 nm; 2) for Radix Paeoniae alba, on silica gel with chloroform – methanol 4:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 4:1 and heating at 105 °C, viewing under daylight; 3) for the fruit of Chinese wolfberry, on silica gel developed with chloroform – ethyl acetate – formic acid 2:3:1, detection at UV 366 nm; 4) for Angelica sinensis, on silica gel with n-hexane – ethyl acetate 17:3, detection at UV 366 nm.

J. of China Pharm. 26 (2), 150-154 (2012). Some health foods were illegally mixed with certain kinds of medicine which may harm the health of consumers. For example antidiabetics like glibenclamide, glipizide and gliclazide were illegaly added to health foods marketed for adjusting blood sugar. Presentation of TLC and UV methods for detection of these illegal additives. TLC on silica gel with chloroform – petroleum ether (60-90 ºC) – methanol – glacial acetic acid 8:14:1:1, detection at UV 254 nm. The methods were applied to three varieties of real life samples and the results showed no difference to those obtained by LC-MS.

Chinese J. of Clin. Res. on Trad. Chinese Med. 4 (9), 34-35 (2012). Langchuang pills are a herbal TCM preparation for clearing heat, removing toxicity and invigorating the circulation of blood. For quality control, TLC of the extracts of the preparations on silica gel 1) for the root of red-rooted salvia, with chloroform – benzene 5:1, detection by viewing in daylight; 2) for Radices Rehmanniae, with chloroform – methanol 8:1, detection by spraying with a saturated solution of 2,4-dinitrophenylhydrazine in ethanol and viewing in daylight; 3) for the rhizome of Chinese Goldthread, with benzene – chloroform – ammonia 5:3:1, detection at UV 366 nm.