CBS 110, 5-7 (2013). HPTLC of extracts of (1) Nauclea diderrichii, (2) Sarcocephalus pobeguinii, (3) Hua gabonii, (4) Morinda lucida, and (5) Momordica foetida on silica gel with A) toluene - ethyl acetate 19:1; B) chloroform - methnaol - water 35:15:2; C) ethyl acetate - acetic acid - formic acid - water 100:11:11:27, D) acetonitrilie - water - formic acid 15:4:1, and E) 1-butanol - acetic acid - water 7:1:2. For (1) mobile phases B and C were best suited, for (2) mobile phase B, for lipophilic compounds of the essential oil of (3) mobile phase A and for (4) and (5) mobile phase B. Bioautographic analysis using alpha- and beta-glucosidase enzym assays, acetylcholinesterase inhibition assay, and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical reagent for detecting radical scavenging activity.

J. of Anhui Coll. of Trad. Chinese Med. 31 (4), 77-79 (2012). Wuzi Yanzong pills are a herbal TCM preparation for treating male impotence, sterility, and premature ejaculation. For quality control, TLC on silica gel 1) for Fructus Lycii and China Dodder, with cyclohexane – acetone 3:2, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis, with chloroform – butanone 30:1, detection under UV 254 nm. Quantification of schisantherin A by absorbance measurement at 230 nm. Linearity was between 0.25-1.10 µg/zone (r = 0.999, n=5). Plate-to-plate %RSD was 2.1 % (n=5) and 0.98 % within plate (n=5). Recovery (by standard addition) was 100.9 % (%RSD = 0.51, n=6).

Jiangxi J. of Trad. Chinese Med. 43 (350), 59-61 (2012). Fufang Sanqidan capsules are a herbal TCM preparation for treating frequent urination, cardiovascular and cerebrovascular diseases, nephropathy etc. For quality control TLC on silica gel for 1) Radix Notoginseng and Radix Astragali, with the upper phase of n-butanol – ethyl acetate – 10 % ammonia 4:1:5, 2) for Salvia miltiorrhiza, with toluene – ethyl acetate 19:1, detection by viewing in daylight; and 3) for Mulberry Fruit, with petroleum ether (30-60 °C) – chloroform – ethyl acetate 3:8:5. All were detectied by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min, viewing under UV 366 nm. Quantification of astragaloside A by HPLC.

Chinese J. Mod. Drug Appl. 7 (1), 118-119 (2013). Zhiheng tablets are a herbal TCM for treatment of hyperlipidemia. For quality control, TLC on silica gel 1) for Radix Notoginseng, with dichloromethane – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Salvia miltiorrhiza and the standard salvianolic acid A sodium, with dichloromethane – acetone – formic acid 25:10:4, detection by exposing to ammonia vapors for 10 min and viewing under UV 366 nm; 3) for Pericarpium Citri Reticulatae and the standard hesperidin, with ethyl acetate – methanol – water 100:17:13 to a distance of 3 cm and then with toluene – ethyl acetate – formic acid – water 20:10:1:1 to a distance of 8 cm, detection by spraying with 3 % aluminium chloride in ethanol and viewing under UV 366 nm; 4) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. of Medication & Clinics 12 (11), 1445-1446 (2012). Xiaocaihu tablet is a herbal TCM for treating chills and fever, dry throat, etc. For quality control TLC on silica gel 1) for Bupleurum chinense, with ethyl acetate – methanol – water 12:2:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % sulfuric acid in ethanol and heating mildly, viewing in daylight; 2) for root of Pilose Asiabell, with n-butanol – glacial acetic acid – ethyl acetate – water 14:2:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C, viewing in daylight.

J. of Fujian Univ. of TCM 22 (5), 52-54 (2012). Coix seed is a TCM herb frequently prescribed to treat edema, dermatophytosis, spasm and diarrhea. For quality control of the crude drug available from different places of origin, TLC of the herb extract and standard glycerol trioleate on silica gel with petroleum ether (60-90 °C) – ethyl acetate – glacial acetic acid 25:5:1, detection by spraying with 0.5 % vanillin in sulfuric acid – ethanol 1:200, heating at 105 °C for 5 min and viewing under UV 366 nm. Coix seed from different places of origin contains different amounts of glycerol trioleate (measured by HPLC).

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 19 (4), 70-71 (2013). Pothos chinensis (Raf.) Merr. and three other medicinal herbs are used in traditional Yao medicine, they have anti venom, anti tumor, and antioxidant effects and reduce blood sugar. For quality control, TLC of 1) Pothos chinensis (Raf.) Merr., on polyamide phase with acetone – water 1:2, detection by spraying with 2 % aluminiumchloride in ethanol and heating at 105°C until the zones are visible in daylight, and under UV 366 nm; 2) for Mappianthus iodoides Hand.-Mazz., on silica gel with petroleum ether (60-90°C) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight, and under UV 366 nm; 3) Zanthoxylum nitidum (Roxb.) DC., on silica gel with chloroform – methanol – ammonia 150:5:1, detection under UV 366 nm; 4) for Saussurea woodiana Hemsl., on silica gel with petroleum ether (60-90°C) – ethyl formate – formic acid 75:25:2, detection under UV 366 nm.

Chinese J. of Med. Guide 10 (17), 98-99 (2012). Banlian Chongji electuary is herbal TCM preparation for the treatment of virus flu and upper respiratory infections. For quality control the indirubin and indigo contents are determined by TLC on silica gel with petroleum ether (60-90 °C) – ethyl acetate – chloroform 1:1:8. Detection in daylight, quantification by densitometry at 290 nm. The linearity was in the range of 0.1-0.5 µg/zone (n=5, r=0.9999) both for indirubin and indigo. The precision (%RSD, n=5) was 1.2 % for indirubin and 2.2 % for indigo. Recovery by standard addition was 95.5 % (%RSD=4.5 %, n=5) for indirubin, and 96.0 % (RSD%=2.9 %, n=5) for indigo.