J. AOAC Int. 88, 1544-1548 (2005). TLC of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol, benzoic acid) on silica gel with 1) ethyl acetate - n-heptane - methanol - diethylamine 30:45:10:2 for bifonazole and benzyl alcohol; 2) n-butyl acetate - n-heptane - methanol - diethylamine 30:45:10:2 for clotrimazole and benzyl alcohol; 3) n-butyl acetate - carbon tetrachloride - methanol - diethylamine 6:12:5:1 for miconazole and benzoic acid. Quantitative determination by reflectance/absorbance measurement at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic caid) and at 210 nm (clotrimazole and benzyl alcohol). Recovery rates for all substances ranged from 98.7 to 100.7 %. The limits of detection and quantitation were 0.03 to 0.2 µg and 0.1 to 0.5 µg/spot, respectively.

J. Planar Chromatogr. 18, 432-436 (2005). TLC of nine benzimidazole and benztriazole derivatives on silica gel impregnated with paraffin oil with methanol - water mixtures. After development the plates were dried and examined under UV light at 254 nm. A quantitative structure-retention relationship (QSRR) correlation study was performed.

Abstract G-25, IPC (2005). HPTLC of cefuroxime axetil in bulk and tablets on silica gel with chloroform - methanol - toluene 2:1:1 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 290 nm. The linearity was within the range of 300-900 ng/spot with an average recovery rate of 99.4 %. The method was validated as per ICH guidelines.

Indian Drugs 42 (8), 511-515 (2005). HPTLC carvedilol in tablets on silica gel with toluene - methanol - ethyl acetate - ammonia 40:10:5:1. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 50-250 µg/µL with recovery of 98.5-100.2 %. Stability was studied during and after development. Accuracy, precision, and specificity of the method were established.

Influence of sample preparation methods and conditions. J. Liq. Chrom. & Rel. Technol. 27, 2463-2470 (2004). TLC of 1-(3,4-methylenedioxyphenyl)-2-nitropropene, an intermediate product of MDMA (3,4-(methylenedioxy)methamphetamine, also known as ,ecstasy’, and impurities on silica gel in a horizontal chamber with chloroform - ethyl acetate 49:1. Detection of separated impurities under UV light at 254 and 366 nm.

Indian J. Pharm. Sci. 67 (2), 182-186 (2005). HPTLC of atorvastatin calcium in its commercial single component tablet formulations (10 mg/tablet), on silica gel with benzene-methanol 7:3. The Rf value was 0.46. Quantitative determination by absorbance measurement at 281 nm. The method was validated in terms of linearity (200-600 ng/spot), precision (intraday variation: 0.25 - 1.01 %, interday variation: 0.21 - 0.88 %), accuracy and specificity. The LOD for atorvastatin calcium was 40 ng/spot, the LOQ was 200 ng/spot. The proposed method was successfully applied to determine atorvastatin calcium content of 10 individual tablet units of two market formulations after extracting atorvastatin calcium with methanol. All formulations were compliant with USP specifications (RSD less than or equal to 6 %) of the content uniformity test. The proposed HPTLC method can analyse ten or more formulation units simultaneously on a single plate and provides a faster and cost effective quality control tool for routine analysis of atorvastatin calcium formulation.

J. Planar Chromatogr. 19, 454-462 (2006). HPTLC of enrofloxazine, norfloxazine, oxytetracycline, trimethoprim, sulfamethazine, sulfadiazine, and penicillin G/procaine on cyano phase with 0.05 M oxalic acid - methanol 81:19. Evaluation under UV light at 254 and 366 nm. Quantitative determination of TMP by absorbance measurement at 254 nm, and fluorescence measurement at 366 nm for the other compounds. The SPE-TLC determination was validated for linearity, precision, quantification, and detection limit.

J. Liq. Chromatogr. Relat. Technol. 28, 2195-2209 (2005). HPTLC of 5’-carbamates of zidovudine (3’-azido-3’-deoxythymidine) and thymidine on RP-18 with methanol - buffer pH 7.4 mixtures with methanol contents between 30 and 80 %; or acetone - buffer mixtures with modifier contents between 20 and 80 % in 5 or 10 % increments. Detection after drying at 40°C developed under UV radiation.