JAOAC Int. 84, 1287-1295 (2001). HPTLC of diazepam, chlordiazepoxide, and midazolam on silica gel with chloroform - benzene - ether - tetrahydrofuran - acetone - acetic acid 35:15:16:10:5:3 in a previously saturated TLC chamber. Detection under UV 254 nm. Quantification by densitometry at 254 nm. The obtained results showed that HPTLC, LC, and GC techniques are comparable for the determination of such benzodiazepines in the requested working range to be analyzed in raw materials.

Indian Drugs 39 (2), 101-105 (2002). HPTLC of piperine and embelin on silica gel G with toluene - ethyl acetate 7:3 and with propanol - butanol - NH3 6:1:3. The method was validated for linearity and recovery. Quantitation by densitometry at 337 nm (piperine) and 333 nm (embelin).

J. Planar Chromatogr. 15, 458-462 (2002). TLC of prenylamine, lidoflazine, bepridil hydrochloride, and fendiline hydrochloride on silica gel, alumina or florisil with several mobile phases comprising mixtures of n-hexane with polar modifiers such as dioxane, ethyl acetate, methanol, ethyl methyl ketone, tetrahydrofuran, and propan-2-ol. Plates were developed in horizontal chambers after conditioning for 15 min and visualized under UV 254 nm with a videoscanning system and by use of different detection reagents. The best separation was achieved on alumina with tetrahydrofuran - hexane 4:1.

J. Liq. Chrom. & Rel. Technol. 26, 2687-2696 (2003). Systematic investigation TLC of 9 phthalazines (e.g. 1-o-bromophenoxy-4-phenyl-phthalazine, 1-p-nitrophenoxy-4-phenyl-phthalazine, and 1-[3-(b-diethylaminoethyl)-4-methyl-7-coumarinyloxy]-4-phenyl-phthalazine and their corresponding 4-tolyl- and -4-benzyl-compounds) on silica gel with toluene - chloroform- methanol 70:20:1. The plates were developed twice with the same mobile phase at room temperature in a saturated N-chamber. Detection under UV 254 nm. The quality of HPTLC-FTIR spectra is sufficient for the identification of unknown substances.

CBS 81, 10-12 (1998). A number of applications of HPTLC in the bulk drug industry are presented: process optimization, fermentation process monitoring, impurity profile, vessel residue certification and bulk drug analysis. The technique is advantageous when a large number of similar samples is to be analyzed and time and cost considerations are of importance.

Part 2. J. Planar Chromatogr. 17, 40-45 (2004). Investigation of the quantitative structure-activity relationships (QSAR) between H1-histaminergic activity and chromatographic data for derivatives of 2-[2-(phenylamino)thiazol-4-yl]ethanamine, 2-(2-benzyl-4-thiazolyl)ethanamine, 2-(2-benzhydrylthiazol-4-yl)ethanamine, 2-(1-piperazinyl)benzothiazole, and 2-(hexahydro-1H-1,4-diazepin-1-yl)benzothiazole. TLC on silica gel, impregnated with solutions of amino acid analogs (propionic acid, propionamide, and n-amylamine) and their mixtures, with acetonitrile - methanol - buffer 2:2:1 and acetonitrile - methanol -dichloromethane - buffer 6:1:1:2. Detection under UV light at 254 nm.

J. AOAC Int. 87, 573- 578 (2004). TLC of propanolol/reagent azo adduct (with diazotized 4-amino-3,5-dinitrobenzoic acid) on silica gel with ethyl acetate - methanol 9:1, chloroform - methanol 4:1, methanol - ammonia 200:3 (plate was impregnated with 0.1 M methanolic KOH before spotting). Evaluation under UV light at 254 nm.

IPC 56th 2004, Abstract No. G-28. Neem Oil obtained from the seed kernels of Azardirachla indica (Meliaceae) is a fixed oil known as oil of Margosa. An HPTLC method is reported for the analysis of Neem oil as a bulk drug and formulations containing oil. TLC of neem oil extracted with chloroform, on silica gel with chloroform - n-hexane - methanol 18:2:1. Quantitative determination by scanning at 254 nm. The linearity range was 100 – 500 mg/mL. Formulations were found to contain 0.35 g/g of Neem Oil.