Journal of Guiyang College of Traditional Chinese Medicine 36 (4), 27-29 (2014). Jianpi Wenwei Jiaonang capsules are a herbal TCM used in the treatment of gastritis. For quality control, TLC for (1) Codonopsis pilosula (Franch.) Nannf., on silica gel with n-butanol – glacial acetic acid – water 14:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are clearly visualized, viewing in daylight; (2) Atractylodes macrocephala Koidz., on silica gel with petroleum ether (60-90 °C) – ethyl acetate 10:1, detection by spraying with 5 % dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:10 and heating until the zones are visualized in daylight; (3) Poria cocos (Schw.) Wolf, on silica gel with toluene – ethyl acetate – formic acid 40:10:1, detection under UV 366 nm; (4) Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y., on silica gel with toluene – acetone 9:2, detection under UV 366 nm; (5) for Salvia miltiorrhiza Bge., on silica gel with toluene – chloroform – ethyl acetate – methanol - formic acid 4:6:8:1:4, detection under UV 254 nm.

Planta Medica 82, 11/12, 1117-1121 (2016). TLC was applied for two purposes: first, to monitor the subfractionation of the methanol extract of the whole Calamus acanthophyllus (eluent and layer not given); second, to analyze the sugar composition of callaphylloside (a weakly cytotoxic pregnastanol saponoside). For this purpose, callaphylloside was heated for 3 h with HCl 2 N under reflux; TLC of the lyophilized and neutralized ethyl acetate extract of the mixture on silica gel with ethyl acetate – n-butanol – water 2:7:1 together with standards. The sugar moiety consisted in rhamnose and glucose (2 units of each, as confirmed through NMR of the compound)._x000D_

Planta Medica 82 (16), 1438-1445 (2016). TLC on silica gel with chloroform – methanol 19:1, followed by detection under UV 254 nm and spraying with 10 % sulfuric acid with heating. TLC was used for 1) the ethyl acetate fraction of a hydromethanolic extract of Eryngium triquetrum aerial parts, and 2) for the monitoring of its fractionation on a cyclodextrin column, and of the fractionation of a selected fraction on silica gel column. The (chloroformic) first subfraction of the last step was found to contain only the polyyne alcohol falcarinol (panaxynol, carotatoxin) at hRF 90.

Rev. Bras. Farmacogn. 27, 13-39 (2017). HPTLC fingerprint of Stevia rebaudiana roots on silica gel with n-hexane – ethyl acetate 19:1. Detection by spraying with 4 % vanillin sulfuric acid reagent, followed by heating at 150 °C for 2–4 min. Chromatographic profiling by TLC and GC–MS analysis showed that roots of plants grown in vitro have the ability to produce secondary metabolites, which can be similar to those produced by plants in nature, but in a sustainable way.

J. Chromatogr. Sci. 54 (7), 1096-1104 (2016). TLC of the extracts of the dried aerial parts of 12 plants of Cirsium species and five selected standards (naringin, apigenin, rutin, caffeic acid and chlorogenic acid) on silica gel with ethyl acetate – formic acid – acetic acid – water 24:3:3:8 on RP-18 phase with 5 % aqueous solution of formic acid – methanol 7:3 for the first development and the same system in the ratio of 1:1 for the second development. Analysis of the results by chemometrics. The comparison of individual Cirsium species and the identification of unknown species by using the similarity indices (Pearson's correlation coefficient, determination coefficient and congruence coefficient), distance indices (Euclidean distance, Manhattan distance and Chebyshev's distance) and Multi-Scale Structural SIMilarity. Based on chemometric analysis, the first extract of the widely grown species is identified as Cirsium arvense and the second one as Cirsium rivulare.

CBS 120, 9-11 (2018). HPTLC of oligomeric proanthocyanidins (PA), alkaloids, and anthocyanins in cocoa (fresh cocoa beans, roasted cocoa, cocoa mass and molded chocolate bars) on silica gel with ethyl formate – formic acid – water – toluene 60:8:6:3 to a migration distance of 70 mm. Detection by immersion in Fast Blue Salt B reagent (140 mg Fast Blue Salt B in 140 mL methanol, 10 mL water, and 50 mL dichloromethane), followed by drying. Evaluation under UV 254 nm and white light. Quantitative determination by absorbance measurement at 280 nm for alkaloids and 510 nm for anthocyanins and derivatized PA. Elution of target zones after derivatization with Fast Blue Salt B directly into the MS, mass spectra were recorded in positive ionization mode. In general, the HPTLC method provides good separation and quick quantification of the target substances. It was found that during fermentation and roasting the levels of the alkaloids and especially oligomeric PAs diminish.

HPLC und DC unter besonderer Berücksichtigung der Prüfungsvorschrift gemäss Ph. EUR. II. ( HPLC and TLC analysis of the rhamnus fallax bark in particular consideration of the identification according to the Ph. EUR. II method.) Dtsch. Apoth. Ztg. 125, 101-105 (1985).TLC of the characteristic xanthorhamnin on silica with ethyl acetate - methanol - water 100:17:13 as a yellow fluorescing spot using the diphenylboryloxyethylamine reagent as a proper medium for the identification of the rhamnus fallax bark.

Biochemical Systematics and Ecology L3, 105-108 (1985). TLC of amentoflavanone, semecarpuflavone, robustaflavanone on silica with benzene - pyridine - formic acid 100:20:7. Detection by spraying with ethanolic FeCl3 solution.