Phytochem. Anal. 24, 395-400 (2013). HPTLC bioautography of the oleo gum-resin galbanum from Ferula gummosa Boiss on silica gel with chloroform - ethyl acetate - methanol 50:5:1 and detection under UV 366 nm. Bioautographic assay to detect the zones inhibiting acetylcholinesterase (AChE). The zones that showed activity in the assay on AChE inhibition were selected for HPTLC-MS. Auraptene and farnesiferol A were identified as AChE Inhibitors.

J. of Inner Mongolia Univ. for Nationalities (Natural Sci. Edit.) 27 (1), 80-83 (2012). Yuzan Qingyan Shiwuwei pills are a traditional Mongolian herbal medicine for treating colds, sore throat, asthma, cough, etc. For quality control, TLC on silica gel 1) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with cyclohexane – formic acetate – formic acid 15:5:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly, viewing in daylight; 3) for Radix Glycyrrhizae and the standard glycyrrhetinic acid, with benzene – petroleum ether (30-60 °C) – ethyl acetate – glacial acetic acid 50:25:20:3, detection under UV 366 nm.

Chinese J. Ethnopharm.(9), 36-38 (2012). Luman-12 is a traditional Mongolian herbal medicine preparation for treatment of cough, asthma, etc. For quality control of the main component herbal drugs TLC on silica gel 1) for Radix Polygalae, with toluene – ethyl acetate – formic acid 28:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Glycyrrhizae, with toluene – ethyl acetate – formic acid 10:8:1, detection under UV 366 nm; 3) for Piper nigrum, with benzene – ethyl acetate – acetone 7:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (8), 2001-2002 (2011). Monkshood and Radix Aconiti agrestis, as traditional Chinese herbal drugs, are usually processed into small pieces ready for decoction and are clinically effective for relieving rheumatic pains. However, in the market some processed drugs were found to be mixed with counterfeits. Presentation of an assay method to investigate the situation of adulteration of these drugs. TLC of the extracts of the drugs 1) for alkaloids, on silica gel with n-hexane – ethyl acetate – methanol 32:18:5 with chamber saturation with ammonia vapors for 20 min, detection by spraying with 5 % potassium iodobismuthate solution. Identification a) by fingerprint comparison with the individual standard component drug; b) by microscopy distinguishing the cluster crystal of calcium oxalate consisted in the thin-wall cells of the drug; 2) for peoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 40:5:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C.

Chinese J. Mod. Drug Appl. 6 (9), 124-125 (2012). Danbaijing capsules are a herbal TCM for treating intractable proteinuria. For quality control, TLC on silica gel 1) for Radix Astragali and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Centella asiatica and the standard asiaticoside, with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Strait Pharm. 24 (9), 53-55 (2012). Huoxiang Qushu Soft capsules are an approved herbal TCM preparation used for promoting perspiration, reducing internal heat, regulating spleen and stomach. For quality control, TLC on silica gel 1) for Herba Pogostemonis, with petroleum ether (30-60 ºC) – ethyl acetate – glacial acetic acid 85:15:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Angelicae Dahuricae, with petroleum ether (30-60 ºC) – diethyl ether 3:2, detection under UV 366 nm; 3) for Folium Perillae, with ethyl acetate – methanol – formic acid – water 18:1:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm; 4) for Rhizoma Atractylodis, with petroleum ether (30-60 ºC) – acetone 9:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 5) for Rhizoma Pinelliae Preparatum, with petroleum ether (30-60 ºC) – ethyl acetate – acetone – formic acid 60:12:10:1, detection under UV 254 nm; 6) for Poria, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight; 7) for Radix Glycyrrhizae, with ethyl acetate – formic acid – glacial acetic acid – water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.

J. of Chinese Med. 26 (169), 716-718 (2012). Shengma Gegen granules are a herbal TCM preparation for the treatment of viral hepatitis type C, drug-induced liver diseases, and postherpetic neuralgia. For quality control, TLC on silica gel 1) for Rhizoma Cimicifugae, with benzene – chloroform – glacial acetic acid 12:2:1, detection under UV 366 nm; 2) for Radix Paeoniae Rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 2 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are visible in daylight. Quantification of puerarin by HPLC.

J. of Modern Trad. Chinese Med. 15 (4), 320-323 (2013). Leifengshi Jiaonang capsules are a herbal TCM preparation for the treatment of rheumatism, ankylosing spondylitis, and lupus erythematosus. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, heating at 105 °C and viewing in daylight and under UV 366 nm; 2) for Angelica sinensis, with petroleum ether (60-90 °C ) – diethyl ether – acetic acid 20:20:1, detection under UV 366 nm 3) for Epimedium sagittatum, with ethyl acetate – formic acid – butanone – water 10:1:1:1, detection under UV 366 nm. Quantification of ferulic acid by HPLC.