J. Agric. Food Chem. 50, 3208-3213 (2002). TLC of plant extracts (e.g. thymol, carvacrol, naphthalinol) on silica gel with chloroform - methanol 9:1 (best resolution) 3:2, n-propanol - water-ethyl acetate 7:1:2, chloroform- methanol - triethylamine 90:9:1, NH3 - butanol 2:5, chloroform - methanol - acetic acid 90:10:1. After drying for 1 h at 37°C, the plates were sprayed with 2 mL of potato-dextrose broth containing a spore suspension of the test organism Cladosporium.

J. Chinese Trad. Patent Med., (Zongchengyao) 25 (2), 125-127 (2003). TLC of isoimperatorin on silica gel with petroleum ether (60-90°C) - ethyl acetate 5:1. Detection under UV. Identification by fingerprint technique. Quantitation by densitometry at 305 nm.

Proc. Intern. Symp. on Planar Separations Plan. Chrom. 203-208 (2003). Description of the novel on-plate solid-liquid continuous extraction (SLCE) with preparative layers. The fine powdered sample to be extracted has to be introduced into a preparative layer on a 200 mm x 30 mm plate which is placed into a special device. The prepared preparative plate is covered with a glass plate, and with the help of a thick filter paper the extraction can be started with the appropriate extraction solvent. HPTLC of furocoumarins on silica gel under unsaturated conditions with ether - dichloromethane - tetrahydrofuran - hexane 10.71:9.68:7.5:72.11. Quantitation by densitometry at 313 nm.

CBS 92, 13-15 (2004). HPTLC on silica gel with toluene - ethyl acetate - methanol - ammonia 25 % 10:10:50:3 over 70 mm with chamber saturation for 30 min. Detection by dipping in iodine solution (0.05 g in 10 mL ethanol 96 %). Visual evaluation under white light. Quantitative determination by absorbance measurement at 210 nm. Repeatability measuring 6 replicates of the same sample on one plate is between 1.6 % and 3.7 %, repeatability of the means from 3 different plates is 1.2 %.

CBS 85, 14-15 (2000). HPTLC on silica gel with ethyl acetate - formic acid - water 45:5:3. Detection by spraying with dichloroquinonechlorimide followed by spraying with 10 % aqueous sodium carbonate solution. Visual evaluation.

J. Planar Chromatogr. 17, 300-304 (2004). TLC of essential oils and thymol, carvacrol, geraniol as standards and streptomycin and gentamycin as positive controls on silica gel with toluene - ethyl acetate 93:7. Detection with ethanolic vanillin sulfuric acid. Quantitative determination at 500 nm. For bioautography the developed plates were dipped for 10 s in approximately 50 mL culture medium containing the test organism followed by drying for 2 min. After storage of the plates at 26 - 28 °C for 17 h they were dipped for 10 s in an aqueous solution (0.1 g / 60 mL) of 3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyltetrazolium bromide (MTT) the layers were incubated at 28 °C for 2 h and then dipped in 70 % ethanol and dried at room temperature.

J. AOAC Int. 88, 1568-1570 (2005). HPTLC of alliin on silica gel with n-butanol - acetic acid - water 3:1:1 at 25 +/- 2 °C and 40 % relative humidity. Detection by dipping in ninhydrin reagent (0.3 g ninhydrin in 100 mL n-butanol and 3 mL acetic acid) for 2 s, followed by heating at 110 °C for 5 min. Quantitative determination by densitometric evaluation of peak areas at 540 nm. Linearity within the range of 250-1500 ng/spot, correlation coefficient of 0.998 and RSD of 2.87 %; mean recovery 98.4 %.

Planta Med. 72, 351-357 (2006). Analytical and preparative TLC of ergosta-4,6,8(14),22-tetraen-3-one, beta-sitosterone, 2,6,11-trimethyldodeca-2,6-10triene, and stigma-4-ene-3,6-dione on silica gel with n-hexane - ethyl acetate 6:1. Preparative TLC of tsangin A with dichloromethane - acetone 25:1; of tsangin B and beilschmien C with dichloromethane - acetone 20:1; of beilschmien A and B with n-hexane - ethylacetate 2:1. Detection under UV light at 254 nm.