Abstracts No. 9156, IHCB (2009). TLC of Hoodia gordonii on silica gel with ethyl acetate - methanol - water 36:7:5. Photodocumentation and estimation was carried out by the DISTA method. The authentication of hang-off capsules, which contained several plant ingredients used to prevent hang-over, was performed by TLC on silica gel with toluene - ethyl acetate 9:1. Detection by spraying with vanillin-sulfuric acid reagent.

J. Chromatogr. B 848 (2), 232-238 (2007). HPTLC of boswellic acids in Boswellia serrata extract and 11-keto-beta-boswellic acid in human plasma on silica gel with hexane - chloroform - methanol 10:10:1. Quantitative determination by absorbance measurement at 260 nm. The linearity range for 11-keto-beta-boswellic acid spiked in 1 mL of plasma was 29 - 146 ng/spot, with a recovery of 91.7 %. The limit of detection and quantification for 11-keto-beta-boswellic acid in human plasma was 9 and 29 ng/mL, respectively. The method was applied for the determination of 11-keto-beta-boswellic acid plasma levels in a clinical pilot study.

60th Indian Pharmaceutical Congress PG-262 (2008). HPTLC of withaferin A in Ashwagandha on silica gel aluminum layer with toluene - ethyl acetate - formic acid 5:5:1. Quantitative determination by absorbance measurement at 214 nm. The chromatograms of the tablet formulation showed a zone corresponding to standard withaferin A (hRf value 37) indicating the presence of the same in the herbal formulation. Linearity was in the range of 50-500 ng/spot. The concentration of withaferin A was 8.07 µg/tablet and 0.00134 % w/w in the tablet formulation.

Abstract No. 91334, IHCB (2009). Standardization of Tinospora cordifolia extract by HPTLC of syringic acid on silica gel with chloroform - methanol 8:1. The hRf value of syringic acid was 53. Quantitative determination by absorbance measurement at 254 nm.

Abstract No. 9274, IHCB (2009). HPTLC of harmalin from ethanolic leaf extracts of Passiflora edulis on silica gel with ethyl acetate - acetic acid - formic acid - water 100:11:11:27. The hRf value of harmalin was 39. The method was linear in the range of 200-1600 ng/spot, recovery was 98.4-99.2 %.

60th Indian Pharmaceutical Congress PG-264 (2008). HPTLC of quercetin in acetone extracts of Psidium guajava leaves on silica gel with toluene - acetone - formic acid 38:10:5. Quantitative determination of quercetin by absorbance measurement at 364 nm. The correlation coefficient was 0.9847. There was a good correlation between peak area and corresponding concentration of quercetin. The proposed HPTLC method provided a good resolution of quercetin from other constituents present in acetone extract of tender leaves of Psidium guajava and can be used for the quantification of quercetin.

J. Pharm. Biomed. Anal. 47, 795-801 (2008). HPTLC of sesamin and sesamoline (the major lignans in sesamum oil and its herbal formulations) on silica gel with benzene - methanol 50:1 with chamber saturation. Quantitative determination by absorbance measurement at 290 nm. The identity of sesamin and sesamoline was confirmed by UV-VIS spectra, NMR and MS of the compounds obtained by scraping off from the plate and elution. For fingerprint analysis derivatization with 5 % methanolic sulphuric acid was performed, followed by heating at 100 °C for 20 min and densitometry at 450 nm.

International Seminar on Herbal Drug Research, PN-011 (2009). HPTLC of harmaline in alcoholic extracts of Peganum harmala on silica gel with chloroform - acetone - diethyl amine 5:4:1 in a saturated chamber at 25 °C. The hRf of harmaline was 50. Quantitative determination by absorbance measurement at 351 nm.