60th Indian Pharmaceutical Congress PG-251 (2008). HPTLC of glycyrrhetinic acid and piperine in haematinic herbomineral capsule formulation on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. Quantitative determination by absorbance measurement at UV 254 nm. The method was linear in the range of 0.8-2.4 ng/spot (glycyrrhetinic acid) and 10-50 ng/spot (piperine). Recovery was 96.3-98.6 %.

60th Indian Pharmaceutical Congress PA-224 (2008). HPTLC of aloe-emodin in extract of Rheum Emodi (prepared by supercritical fluid extraction) on silica gel with toluene - acetone - formic acid 80:20:1. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 100-400 ng/spot. Compared with other extraction techniques supercritical fluid extraction was more effcient and less time consuming.

60th Indian Pharmaceutical Congress PG-258 (2008). HPTLC of isovitexin in Passiflora incarnata raw material and extract on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. The plant was found to contain 0.087 % w/w of isovitexin.

Abstract No. 9712, IHCB (2009). HPTLC of extracts from leaves of Dendrophthoe falcata on silica gel with methanol - formic acid - water 40:3:57. Quantitative determination by absorbance measurement at 280 nm using quercetin as marker.

J. Planar Chromatogr. 22, 367-369 (2009). HPTLC of triterpenoids (alpha- and beta-amyrin, oleanolic acid) on silica gel prewashed with methanol and dichloromethane, with dichloromethane - ethyl acetate 37:3. in a horizontal chamber saturated for 15 min. Detection by spraying with 8 % sulfuric acid in ethanol and heating at 105 °C for 3 min. Evaluation in daylight and under UV 366 nm. Quantitative determination by absorbance measurement at 520 nm.

J. AOAC Int. 93, 1617-1621 (2010). HPTLC of aconitine on silica gel with ethyl acetate - ethanol 3:1 at 22 °C in a saturated twin-trough chamber. The hRf value of aconitine was 33. Quantitative determination by absorption measurement at 238 nm. LOD and LOQ was 20 and 70 ng/band, respectively. The linearity with respect to peak area was in the range of 300 to 1800 ng/band with an r of 0.9991. The repeatability (RSD) was 0.85 %; and the inter-day and intra-day precision (RSD) was 1.01-1.38 and 1.04-1.34 %, respectively.

Abstract No. C-497, 61st IPC (2009). HPTLC of ellagic acid in seeds of Eugenia jambolana Lam on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 100:11:11:27. Densitometric evaluation at 254 nm. The method was linear in the range of 200-1200 ng/band. The alcoholic seed extract contained 11.03 % of ellagic acid and 21 % of total tannin (measured by chemical method).

Indian Drugs 47(6), 31-38 (2010). Pods of Prosopis were extracted with different solvents and the extracts were subjected to phytochemical evaluation using 2D paper chromatography and HPTLC. Analysis of phenolic acids by two-directional paper chromatography with toluene - acetic acid - water 6:7:3 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. For detection of phytoconstituents HPTLC on silica gel with different mobile phases and detection wavelengths: 1) beta-sitosterol with toluene - methanol 9:1, measured at 258 nm, 2) coumaric acid with toluene - ethanol 4:1, measured at 292 nm, 3) quercetin with toluene - methanol 4:1, measured at 271 nm, and 4) caffeine with toluene - acetone 7:3, measured at 272 nm.