Abstract No. F-262, 61st IPC (2009). HPTLC of L-dopa on silica gel with n-butanol - water - actic acid 4:1:1. The hRf was 37. Detection by spraying with 0.5 % ethanolic ninhydrin solution, followed by heating at 120 °C for 2 min. Quantitative determination by absorbance measurement at 520 nm. The method was linear in the range of 600-1400 ng/band.

Chinese J. Hospit. Pharm. 29 (4), 336-338 (2009). TLC of astragaloside in Tianjian capsules on silica gel with chloroform - methanol - water 13:6:2. Detection by spraying with 5 % sulfuric acid in ethanol followed by heating at 105 ºC until coloration. Quantification by densitometry at 515 nm. The linearity was between 0.98 and 4.90 µg/spot (r2 = 0.998), the %RSD was 2.4 % (n = 6) within plate and 2.3 % (n = 6) inter-plate, standard addition recovery was 99.2 % with RSD = 1.7 % (n = 6).

J. Planar Chromatogr. 23, 112-115 (2010). HPTLC of phyllanthin on silica gel (prewashed with methanol) with hexane - toluene - ethyl acetate 2:2:1 in a twin-trough chamber saturated at 25-30 °C and 40-50 % relative humidity. Quantitative determination by absorbance measurement at 206 nm. LOD was 70 ng/mL, LOQ 200 ng/mL.The linear calibration range was 200-1200 ng/mL. Repeatability (RSD, n = 3) was 0.18-0.59 % with a correlation coefficient of 0.999. Intra-day and inter-day precision studies showed the CV was less than 2.0 %, indicating the method was precise: %RSD at 200, 600, and 1200 ng/mL was between 0.43 and 1.51 % for intra-day precision, and between 0.59 and 1.73 % for inter-day precision. The intra-day recovery for 200, 600 and 1200 ng/mL was between 102.3 % and 99.9 %, and the inter-day recovery between 102.5 % and 99.9 %, respectively.

Abstract No. C-37, 61st IPC (2009). HPTLC of methanolic leaf extracts of Aloe vera (after purification with petroleum ether (60-80 °C)) on silica gel with toluene - ethyl acetate - glacial acetic acid - methanol 4:18:1:4. Under UV 254 nm six bands with hRf values of 12, 26, 34, 44, 62, and 84 were observed. These bands correspond to well known constituents of Aloe vera: aloeresin hRf 25, barbaloin hRf 33, aloe emodin hRf 43, emodin hRf 63. The bands with hRf values of 12 and 84 could not be identified. The reported finger print profiling can serve as potential technique for authentification and batch to batch consistency of herbal drugs.

Journal of Natural Remedies 10(2), 170-174 (2010). The powdered plant material was defatted, extracted with methanol, concentrated and successively extracted with ethyl acetate and n-butanol. The resulting fractions were concentrated and subjected to chromatographic fingerprint analysis of the phytoconstituents, i.e. alkaloids, saponins, tannins, flavonoids, anthraquinones and sterols. TLC on silica gel with n-butanol - acetic acid - water 4:1:5 for the ethyl acetate fraction and with chloroform - methanol 9:1 for the n-butanol fraction. Densitometric evaluation at 254 nm and 366 nm for the n-butanol fraction and at 290 nm for the ethyl acetate fraction. Derivatization with vanillin-sulfuric acid reagent for the n-butanol fraction and with AlCl3 reagent for the ethyl acetate-fraction, evaluation at 600 nm. The n-butanol fraction contained sterols and saponins, the ethyl acetate fraction flavonoids.

Modern J. of Integrated Trad. Chinese & Western Med. 19(31), 3439-3441 (2010). TLC on silica gel with chloroform – methanol – water 13:7:2. Detection by spraying with 10 % slfuric acid in ethanol and heating at 105 °C until the zones are visualized, evaluation under UV 366 nm. Identification of the component drugs Radix Astragali and Rhizoma Chuanxiong P.E by comparison of the retention values and color of the zones by the active compounds astragaloside and ferulic acid in the individual drug.

J. of Qilu Med. & Pharm. 29(11), 658-659 (2010). TLC on silica gel with 1) benzene – methanol 27:1; 2) toluene – methanol 17:1 ; 3) cyclohexane – propanone 10:3:4) petroleum ether (60-90 ºC) – ethyl acetate – formic acid 85:15:2, or 80:20:1. Detection by spraying with 1 % vanillin in sulfuric acid and heating at 100 °C until the zones were visualized. Identification by comparison of the fingerprint with the characteristic reference standards magnolol and honokiol. System 4) provided the best separation.

J. Planar Chromatogr. 24, 394-399 (2011). TLC of leave extracts and six flavonoids as markers (vitexin, isovitexin, orientin, isoorientin, quercetin, and tricin) on silica gel, prewashed with methanol and methylene chloride, with methanol - ethyl acetate - acetone - methylene chloride in different ratios using automated multiple development. The developed plate was dried in air for 2 h and sprayed with 1 % aluminum trichloride in ethanol. Then the plate was left for 2 h for derivatization in a glass drying chamber. Quantitative determination by densitometry at 366 nm. The hRf values of the six marker flavonoids were 22, 31, 38, 45, 57, and 88, respectively. Linearity was between 175 and 1750 ng/band. Instrument precision (n = 10) was between 0.2-0.9 %. The repeatability for standards and samples (n = 9), was 0.7 and 0.5, 0.8 and 0.5, 0.8 and 0.5, 0.8 and 0.4, 1.3 and 0.7, 1.1 and 0.3 % for isoorientin, orientin, isovitexin, vitexin, quercetin, and tricin, respectively. The limits of detection were 35, 40, 35, 50, 80, and 20 ng/zone for isoorientin, orientin, isovitexin, vitexin, quercetin, and tricin, respectively. The intra-day and inter-day precision was between 0.1-2.9 % and 0.3-2.4 % for all six marker flavonoids.