Abstract No. F-10, 61st IPC (2009). HPTLC of Embella ribes Churna formulation on silica gel with chloroform - ethyl acetate - formic acid 5:4:1 in a twin trough chamber. Densitometric measurement of embelin at 291 nm. The method was linear in the range of 600-1800 ng/band with recovery value of 99.1-101.2 %. The formulation was also analyzed by HPLC and results were found to be comparable.

Abstract No. C-258, 61st IPC (2009). HPTLC of piperine, plumbagine and zingiberine in Panchkol Churna, an ayurvedic preparation used for anorexia, distension and abdominal pain. HPTLC on silica gel with toluene - ethyl acetate 7:3. Densitometric evaluation at 340 nm for piperine, and at 420 nm for plumbagine and zingiberine. The hRf value of piperine, zingiberine and plumbagine was 31, 75, and 84.

International Seminar on Herbal Drug Research, PN-024 (2009). HPTLC of catechins and epicatechin in leaves of tea (Camellia sinensis on silica gel with toluene - ethyl acetate - formic acid 7:5:1. Quantitative determination by absorbance measurement at 254 nm. The leaves were found to contain 10-24 % of total polyphenols.

International Journal of ChemTech Research 2(2), 807-812 (2010). Chloroform and ethyl acetate extracts of leaves and bark of Ficus mollis (Moraceae) were subjected to TLC fingerprint profiling on silica gel with toluene - ethyl acetate - formic acid 16:2:1. Evaluation under UV 254 nm. Derivatization with vanillin-sulfuric acid reagent, followed by heating at 105 °C until colorization. In the bark 7 well-defined zones were observed, whereas in leaves 10 zones were observed. Heavy metal and mineral analysis was performed by atomic absorption spectroscopy.

Acta Chromatographica 22 (2), 297-305 (2010). Description of a simple, precise, and accurate method for simultaneous quantification of aspirin, atorvastatin calcium and clopidogrel bisulphate by HPTLC on silica gel with toluene – methanol - formic acid 65:35:1. The hRf values were 26, 47, and 78 for aspirin, atorvastatin calcium, and clopidogrel bisulphate, respectively. Quantification by densitometry at 254 nm. The precision intra-day and inter-day was in the ranges of 0.2–0.7 %RSD and 0.5–1.0 %RSD for aspirin, 0.4–0.9 %RSD and 0.4–0.6 %RSD for atorvastatin calcium, and 0.3–0.7 %RSD and 0.4–0.9 %RSD for clopidogrel bisulphate.

J. Sep. Sci. 34, 286-291 (2011). HPTLC of 1beta,3alpha,8beta-trihydroxy-pimara-15-ene (1), 6alpha,11,12,16-tertahydroxy-7-oxo-abieta-8,11,13-triene (2) and 2alpha,19-dihydroxy-primara-7,15-diene (3) in the root bark of Premna integrifolia on silica gel with hexane – acetone – ethyl acetate 3:1:1. Detection by dipping into vanillin-sulfuric acid reagent (2 g vanillin in 190 mL ethanol with 10 mL sulfuric acid) followed by air drying and heating for 3 min at 110 °C. Quantitative determination by absorbance measurement at 475 nm. The hRf values of (1), (2) and (3) were 58, 44, and 32, respectively and selectivity regarding matrix was given. Linearity was between 1-10 µg/spot for (1), (2) and (3), respectively. The limits of detection were found to be 230, 106 and 336 ng/band for compounds (1), (2) and (3), respectively, whereas the limits of quantification were 769, 354 and 1122 ng/band, respectively. Inter- and intraday precisions were 0.9-1.3 % and 1.1-1.2 %, respectively. The average recoveries for compounds (1) to (3) were found to be 100.6, 103.9 and 97.6 %, respectively, within the acceptable %RSD.

PHCOG J. 2(11), 381-385 (2010). TLC of Tylophora indica leaves, extracted with petroleum ether, chloroform and methanol, on silica gel with 1) n-hexane - ethyl acetate 2:3 for the petroleum ether extract, 2) chloroform - ethyl acetate - methanol 18:1:1 for the chloroform extract, and 3) chloroform - toluene - ethyl acetate 18:1:1 for the methanolic extract. Evaluation under UV 254 nm and 366 nm. 12 prominent bands were observed in all chromatographic fingerprints. The method is suitable for identification and authentication of the plant material.

Planta Med. 74, 352-353 (2008). HPTLC of tetraphyllin, turneradiffusin, beta-arbutin, terniflorin, echinaticin, turneradin and methanolic extracts of Turnera diffusa on silica gel with ethyl acetate - acetic acid - water 190:10:1. Quantitative determination by densitometric absorbance measurement at 254 nm.