J. of China Pharm. 23 (2), 48-51 (2014). Qingbu Tongluo Wan pill is a herbal TCM preparation for the treatment of acute and chronic brucellosis. For quality control, TLC on silica gel (1) for Fructus aurantii, with chloroform – formic acid – water 13:6:2, detection by spraying with 3 % aluminium chloride in ethanol and heating at 105 ºC, detection under UV 366 nm; (2) for Salvia miltiorrhiza Bge., with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC, evaluation in daylight; (3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposing to iodine vapors for 3 min, evaluation under UV 365 nm; (4) for Radix paeoniae rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zonesare visible in daylight. Quantification of salvianolic acid B by HPLC.

Planta Medica 82 (15), 1368-1373 (2016). The incubation mixture of dihydroergotamine with rat liver microsomes was dissolved in methanol – chloroform – ethyl acetate 1:1:1. Preparative TLC on silica gel with methanol – chloroform 1:9. Two zones (hRF 21 and 35) corresponding to produced metabolites were scratched off the plate, separated from the silica with methanol – ethyl acetate 1:1 and, after purification on cyclodextrin, identified through LC-MS-MS and proton-NMR as 5-hydroxyl-dihydroergotamine and 11-hydroxyl-dihydroergotamine, respectively.

Planta Medica 83(01/02), 135-142 (2017). Preparative TLC on silica gel for the purification of recrystallized farnesiferol B (hRF 50; with petroleum ether – ethyl acetate 8:1) and sinkianol B (hRF 40; with chloroform – methanol 15:1) isolated through column chromatography from petroleum ether – dichloromethane fractions of the chloroform extract of Ferula sinkiangensis gum resin.

J. AOAC Int. 99, 1204-1212 (2016). HPTLC of St. John’s wort labeled as Hypericum perforatum on silica gel with ethyl acetate – dichloromethane – formic acid – acetic acid – water 100:25:10:10:11 (USP 38-NF33), ethyl acetate – formic acid – water 30:2:3 (European Pharmacopoeia) and ethyl acetate – acetic acid – formic acid – water 100:11:11:26 (USP 37-NF32). For the investigation of synthetic dyes, samples were analyzed on RP-18 with methanol – 5 % aqueous sodium sulfate 3:4. Detection by dipping into natural products reagent (1 g diphenylborinic acid 2-aminoethylester in 200 mL ethyl acetate), then in polyethylene glycol reagent (10 g PEG 400 (macrogol) in 200 mL dichloromethane). Qualitative identification under UV 254 and 366 nm. A decision tree was developed to analyze adulterated samples labeled as Hypericum perforatum. If for example under UV 366 nm after derivatization a yellow fluorescent zone is seen at hRF 46, samples are probably mixed with another undesired species. In addition, if a bluish zone is seen at application position of samples identified with the test USP 38-NF32 and examined under white light prior derivatization, the sample is probably mixed with dyes.

Planta Medica 83(14/15), 1176-1183 (2017). The fractionation on cyclodextrane with methanol – water 9:1 of the acetone macerate of Crocus sativus flowers (without stigma) was analyzed by TLC on silica gel with ethyl acetate – acetic acid – formic acid – water 100:11:11:26. Detection under UV light and after derivatization with natural products reagent (1 % in methanol), followed by 5 % ethanolic PEG solution. The 182 fractions were pooled into 8 fractions, whereby fraction 2 contained pure kaempferol-3-O-sophoroside (its structure and 98 % purity were confirmed by NMR). The yield was 1.4 % of the dried flowers._x000D_

of Pharm.(Yaoxue Xuebao) 20, 59-66 (1985). (Chinese). (HPLC and TLC determination of alkaloid constituents in sophara flavescens and saphora lopecuroides.) TLC of sophocarpine, matrine, sophoridine, oxymatrine and oxysophocarpine on silica with benzene - acetone - methanol 8:3:0.5. Determination by densitometry at 510 nm. Comparison with HPLC results (good agreement).

Evaluation of the constituents and quality.) HPTLC of tetrahydrocoptisine, alpha-corydaline, DL-tetrahydropalmatine, protopine, coptisine, palmatine and glaucine on silica with various developing systems.

by thin-layer chromatography.) TLC of hyperoside on silica with ethyl acetate - butanol - formic acid - water 5:3:1:1, or on polyamide with chloroform - methanol - water 4:1.5:0.1. Detection by spraying with 1 % aluminium chloride. Elution with methanol - water - ethyl acetate 1.5:1:1. Quantification by pulse polarography.