J. Planar Chromatogr. 9, 286-288 (1996). HPTLC of 3,4-methylenedioxyamphetamines of the ecstasy group (MDA - 3,4-methylenedioxyamphetamine, MDMA - N-methyl-3,4-methylenedioxyamphetamine, MDE - N-ethyl-3,4-methylenedioxyamphetamine) on silica with ethyl acetate - acetone - methanol - 25% NH3 10:10:4:1. After careful removal of the mobile phase, NH3 in particular, derivatization with OBPB solution (26.3 mg in 100 ml acetone or methanol) by dipping the chromatogram three times, 2 s each. The plate was heated at 100°C for 5 min for stabilization. The reagent solution is stable for several months if stored in the refrigerator protected from light. Densitometry before derivatization at 283 nm and after derivatization at 355 nm; recording of spectra.

J. Planar Chromatogr. 13, 146-148 (2000). TLC of the bitterant denatonium saccharide on silica gel with ethanol - methanol - butanol - hydrochloric acid 18:48:18:0.05 in a presaturated chamber. Detection after drying by exposure to chlorine gas for ca 5 min. After removal of all excess chlorine, the plate was uniformly sprayed with o-tolidine reagent and then with 1% phosphomolybdic acid. The detection limit for denatonium saccharide is 0.2 µg per spot.

J. Liq. Chromatogr. & Relat. Technol. 29, 2587-2592 (2006). TLC of solanesol on silica gel with petroleum ether - ethyl acetate 4:1. Detection by spraying with sulfuric acid - anisaldehyde - glacial acetic acid 5:5:90, followed by at 110 °C.

59th Indian Pharmaceutical congress C-299, 296, (2007). The gastroprotective drug Gastse was prepared by mixing standardized alcoholic extracts of Capsicum annum, Chelidonium majus, Strychnos nux vomica, and Arsenicum album. HPTLC of Gastse on silica gel with n-hexane - ethyl acatate 52:6. Evaluation at UV 254 nm and 366 nm. Densitometric determination at 200 nm and 400 nm.

J. Pharm. Biomed Anal. 46(2), 391-394 (2008). HPTLC of conessine silica gel aluminum plates with toluene - ethyl acetate - diethyl amine 13:5:2 in a twin trough chamber saturated with mobile phase at 25 °C. Detection by spraying with modified Dragendorff's reagent. Quantification by densitometry in absorbance mode at 520 nm. Linearity was between 1 and 10 µg/zone (r2 = 0.9998).

Food Chem. 79, 337-342 (2002). HPTLC of polyphenol compounds (caffeic acid derivatives, quercetin and kaempferol glycosides) in the leaves of Lactuca sativa on RP-18 with water – methanol – acetic acid 25:25:3. Detection by dipping into a solution of 1 % ethanolamine diphenylborate in methanol for 24 h. Quantitative determination by absorbance measurement at 365 and 440 nm. The total flavonoid amount is expressed as isoquercitrin using a three point regression curve in the range of 1 and 5000 ng/zone.

Pharmazie 63, 405-407 (2008). TLC of (+)-haemanthamine on silica gel or on RP-18. Detection by spraying with Dragendorff reagent or with 1 % cerium sulfate - sulfuric acid, followed by heating.

Phytochem. Anal. 19, 236-243 (2008). HPTLC of curcumin (1), demethoxycurcumin (2), and bisdemethoxycurcumin (3) in the rhizome of Curcuma longa L. on silica gel with toluene - acetic acid 4:1 for curcuminoid separation and n-hexane - ethyl acetate - acetic acid 16:5:1 for quantification of total curcuminoid content. Quantitative determination by absorbance measurement at 254 nm. To determine the free radical-scavenging activity of individual compunds, the plate was dipped into a 0.5 mM solution of 1,1-diphenyl-2-picrylhydrazyl (DPPH radical) in methanol for 5 s, dried in darkness at room temperature and heated at 60 °C for 30 s. Quantitative determination by absorbance measurement at 517 nm as negative peaks. The hRf values were 50, and 25 for (1) and (3), respectively. Linearity was between 80 and 250 ng/spot for (1), 40 and 280 ng/spot for (2), and 85 and 270 ng/spot for (3). The limits of detection and quantification were 20 and 60 ng/spot for (1), 10 and 30 ng/spot for (2), and 25 and 75 ng/spot for (3). Recoveries were 99.0 %, 96.9 %, and 95.8 %, (reference value 80 %), respectively. The intermediate/interday/intra-day precision for (1), (2), and (3) was 3.15 %, 3.00 %, and 2.85 %, (n=6), respectively. For the free radical-scavenging activity, linearity was between 70 and 400 ng for (1), 50 and 550 ng for (2), and 60 and 250 ng for (3). The limits of detection and quantification were 40 and 70 ng for (1), 25 and 50 ng for (2), and 45 and 60 ng for (3). The intermediate/interday/intra-day precision for (1), (2), and (3) was 6.50 %, 2.30 %, and 1.50 % (n=6), respectively.