J. of Jinan Univ. (Natural Sci.) 33 (3), 289-293 (2012). Compound Shuyu granule is a herbal TCM preparation prescribed clinically to treat insomnia, neurosis and melancholia. TLC of the extracts of the medicine on silica gel 1) for Caulis Polygoni Multiflori and the standard emodin, with the upper phase of petroleum ether (60-90 ºC) – formyl acetate – formic acid 15:5:1, detection under UV 366 nm and by exposiure to iodine vapors and viewing under UV 366 nm; 2) for Salvia miltiorrhiza and the standard salvianolic acid B, with cyclohexane – dichloromethane – acetone – formic acid – glacial acetic acid 2:4:2:2:1, detection by spraying with 5 % ferric chloride in ethanol and heating at 105 ° until the zones are visible; 3) for white Paeony root, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly until the zones are seen.

Chinese J. of Herald of Med. 31 (10), 1344-1347 (2012). Compound Bibao tablet is a herbal TCM preparation prescribed clinically to treat chronic rhinitis, nasosinusitis and migraine. For quality control a method for the identification of the main ingredients and determination of volatile oil in the preparation has been established. TLC on silica gel 1) for Licorice, with ethyl acetate – acetic acid – water 18:2:1 with chamber saturation with the mobile phase for 15 min, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly until the zones are visible, evaluation in daylight and under UV 366 nm; 2) for Asarum sieboldi Mig., with petroleum ether (60-90 ºC) - ethyl acetate 3:1 with chamber saturation with the mobile phase for 15 min, detection at UV 254 nm; 3) Flos Magnoliae liliflorae Buds, with petroleum ether (60-90 ºC) – ethyl acetate – formic acid 15:5:2 with chamber saturation with the mobile phase for 15 min, detection under UV 366 nm.

J. of China Pharm. 21 (3), 22-23 (2012). Rubingxiao tablet is a herbal TCM preparation prescribed to treat mastitis and hyperplasia of mammary glands. Quality control by qualitative identification of its major component drugs and quantitative determination of tetrahydropalmatine in it. TLC of the extracts of the preparations on silica gel 1) for Epimedium davidii Franch, with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection at UV 366 nm, followed by spraying with 1 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Astragalus mongholicus, with chloroform – methanol 10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visible, evaluation at UV 366 nm; 3) for Poria cocos, with toluene – ethyl acetate – formic acid 40:10:1, detection by spraying with 2 % vanillin in sulfuric acid - ethanol 1:4 and heating at 105 °C until the zones were visible, viewing under daylight.

J. of World Trad. Chinese Med. 7 (1), 77-78 (2012). Yiqi Shenggu granule is a newly developed herbal TCM preparation for treating wounded catagma. TLC of the extracts of the preparation on silica gel 1) for Aucklandia root, with petroleum ether (60-90 ºC) – ethyl acetate 9:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and mild heating, viewing in daylight; 2) for Carthamus flower, twice with chloroform – toluene – methanol 15:5:1, detection by spraying with 10 % sulfuric acid in ethanol and viewing at UV 366 nm; 3) for red Peony root, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 200:1 and mild heating, viewing in daylight. Determination of astragaloside A in the extract of the medicine by TLC on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and viewing at UV 366 nm. Quantification by densitometry at 520 nm with a linearity range of the calibration curve of 1.13-6.75 µg/zone (r = 0.995, n = 6), a precision of %RSD = 2.0 (n = 6) within plate and plate-to-plate, and standard addition recovery of 99.4 % (%RSD = 2.3, n = 6).

Phytochem. Anal. 24, 395-400 (2013). HPTLC bioautography of the oleo gum-resin galbanum from Ferula gummosa Boiss on silica gel with chloroform - ethyl acetate - methanol 50:5:1 and detection under UV 366 nm. Bioautographic assay to detect the zones inhibiting acetylcholinesterase (AChE). The zones that showed activity in the assay on AChE inhibition were selected for HPTLC-MS. Auraptene and farnesiferol A were identified as AChE Inhibitors.

J. of Inner Mongolia Univ. for Nationalities (Natural Sci. Edit.) 27 (1), 80-83 (2012). Yuzan Qingyan Shiwuwei pills are a traditional Mongolian herbal medicine for treating colds, sore throat, asthma, cough, etc. For quality control, TLC on silica gel 1) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with cyclohexane – formic acetate – formic acid 15:5:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly, viewing in daylight; 3) for Radix Glycyrrhizae and the standard glycyrrhetinic acid, with benzene – petroleum ether (30-60 °C) – ethyl acetate – glacial acetic acid 50:25:20:3, detection under UV 366 nm.

Chinese J. Ethnopharm.(9), 36-38 (2012). Luman-12 is a traditional Mongolian herbal medicine preparation for treatment of cough, asthma, etc. For quality control of the main component herbal drugs TLC on silica gel 1) for Radix Polygalae, with toluene – ethyl acetate – formic acid 28:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Glycyrrhizae, with toluene – ethyl acetate – formic acid 10:8:1, detection under UV 366 nm; 3) for Piper nigrum, with benzene – ethyl acetate – acetone 7:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (8), 2001-2002 (2011). Monkshood and Radix Aconiti agrestis, as traditional Chinese herbal drugs, are usually processed into small pieces ready for decoction and are clinically effective for relieving rheumatic pains. However, in the market some processed drugs were found to be mixed with counterfeits. Presentation of an assay method to investigate the situation of adulteration of these drugs. TLC of the extracts of the drugs 1) for alkaloids, on silica gel with n-hexane – ethyl acetate – methanol 32:18:5 with chamber saturation with ammonia vapors for 20 min, detection by spraying with 5 % potassium iodobismuthate solution. Identification a) by fingerprint comparison with the individual standard component drug; b) by microscopy distinguishing the cluster crystal of calcium oxalate consisted in the thin-wall cells of the drug; 2) for peoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 40:5:10:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C.