J. Trad. Chinese Veterinary Med. 55 (1), 15-19 (2021). Qixingcha Keli is a TCM preparation converted from human to animal use, for the treatment of gastric stagnation in piglets and other animals. Quality control of its component (A) Crataegus L. by TLC of the ethanol extracts, the control solutions with and without Crataegus L. on silica gel with ethyl ether - chloroform - formic acid 5:5:1 to 9 cm. Detection by heating the plates at 105 ˚C for 15 min, then spraying with 0.05 % bromophenol solution (pH = 7.5) and viewing in white light. For component (B) Glycyrrhiza uralensis Fisch., TLC of the ethanol extracts, the control solutions with or without Glycyrrhiza uralensis Fisch. on silica gel containing 1 % NaOH with ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:1 to 18 cm, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ˚C, evaluation in white light and at UV 365 nm. For (C) Uncaria rhynchophylla (Miq.) Miq. ex Havil., TLC of the ethanol extracts and the control solutions on silica gel with petroleum ether (60-90 ˚C) - propanone 3:2 to 9 cm, detection at UV 254 nm. For (D) Semen Coicis, by TLC of the ethyl acetate extracts and control solutions on silica gel with petroleum ether (60-90 ˚C) - ethyl ether - glacial acetic acid 166:34:1 to 9 cm, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:200 and heating at 105 ˚C, detection in white light and at UV 365 nm.

J. Food Compos. Anal. 93, 103616 (2020). HPTLC profile of a selected herbal dietary supplement containing seven herbal components as ingredients on the product label (capsicum, cactus, wheat, white bean, Garcinia cambogia, psyllium husk and black pepper) on silica gel with hexane - ethyl acetate 2:1. Detection by spraying with Dragendorff’s reagent or 10 % potassium hydroxide solution in ethanol for the detection of alkaloids and anthraquinones, respectively. Qualitative analysis under UV light at 254 and 366 nm. Further analysis by mass spectrometry. The method allowed the identification of contaminant species, including the hidden oleamide compound within the selected herbal product.

Anal. Bioanal. Chem. 411, 6655-6665 (2019). HPTLC of lovastatin present in lactone (1) and hydroxy acid forms (2) and mycotoxin citrinin (3) in red yeast rice on silica gel with n-hexane - acetone - 10 % acetic acid 60:40:1. Quantitative determination by absorbance measurement at 238 nm for (1) and (2) and fluorescence measurement at 313/> 400 nm for (3). The hRF values for (1) to (3) were 35, 23 and 7, respectively. Linearity was between 50 and 500 ng/zone for (1) and (2) and 5 and 50 ng/zone for (3). Intermediate precision was below 2 % (n=5). The LOD and LOQ were 10 and 50 ng/zone for (1) and (2) and 1 and 4 ng/zone for (3). Average recovery was 109.9 % for (1) to (3).

Anal. Bioanal. Chem. 411, 725-734 (2019). HPTLC of bisphenol A (1) and its nine brominated analogs, namely monobromobisphenol A (2), 2,2’-dibromobisphenol A (3), tribromobisphenol A (4), tetrabromobisphenol A (TBBPA) (5), TBBPA mono(methyl ether) (6), TBBPA mono(allyl ether) (7), TBBPA mono(2,3-dibromopropylether) (8), TBBPA bis(allyl ether) (9), TBBPA bis(2,3-dibromopropyl ether) (10) in chicken samples on silica gel with n-hexane - ethyl acetate - dichloromethane - acetic acid 25:5:5:1. Detection at UV 254 nm. Further analysis by high-performance liquid chromatography-diode array detector/triple quadrupole mass spectrometry. The hRF values for (1) to (10) were 30, 32, 33, 45, 58, 68, 69, 69, 91 and 86, respectively.  

J. Sep. Sci. 44, 530-538 (2021). HPTLC of tamsulosin (1) and tadalafil (2) in presence of their degradation products (2) and (3) on silica gel with ethyl acetate - toluene - methanol - ammonia 20:10:20:3. Quantitative determination by absorbance measurement at 280 nm. The hRF values for (1) to (4) were 55, 68, 14 and 24, respectively. Linearity was between 0.5 and 25 µg/zone for (1) and (2). Intermediate precision was below 2 % (n=3). Average recovery was 99.7 % for (1) and 100.3 % for (2).

Pharm. Res. 38, 127-140 (2021). HPTLC of [11C]metoclopramide in plasma, kidney, urine and liver extracts on silica gel with ethyl acetate - ethanol - 25 % ammonium hydroxide 16:4:1. Detection using a multisensitive phosphor screen. The hRF value for [11C]metoclopramide was 60. The method allowed kinetic analysis in different organs after radiotracer injection in mice.

J. Liq. Chromatogr. Relat. Technol. 44, 87-94 (2021). HPTLC fingerprinting of Selinum
tenuifolium on silica gel with toluene - ethyl acetate - formic acid 13:11:2. Detection under UV light at 254 nm and 366 nm. The hRF values for gallic acid and ferulic acid were 36 and 58, respectively. 

J. Planar Chromatogr. 34, 89-94 (2021). HPTLC of fresh Kakadu plum fruits (Terminalia ferdinandiana) on silica gel with toluene - ethyl acetate - formic acid 5:3:2. Detection by dipping into natural product reagent (1.0 g of 2-aminoethyl diphenylborinate in 100 mL methanol), followed by heating at 100 ºC for 3 min. Quantitative determination by absorbance measurement at 366 nm. The HPTLC fingerprint allowed to differentiate between different sources of fruit.