Part II: Quantitation and evaluation of ceftriaxone J. Planar Chromatogr. 11, 258-262 (1998). HPTLC of ceftriaxone (without prior solvent elution) on reversed-phase hydrocarbon-impregnated silica gel with water for plate washing and as spotting solvent. Evaluation of the spots at different wavelengths by densitometry. The direct sample determination (DSD), i.e. sample spotting coupled with densitometric quantitation, has a great advantage over GC or HPLC when the need for separation is absent and high sample throughput is required.

J. Planar Chromatogr. 11, 428-432 (1998). New TLC method HPTLC-OPLC and conventional TLC on silica gel with n-hexane - butyl acetate - ethyl acetate - chloroform 12:3:3:4; OPLC, and 6:3:3:4, TLC. Evaluation at 233 nm by densitometry. Development and validation of a quantitative OPLC purity test for phthaloyl-amlodipine and comparison of the validation characteristics (specificity characterized by retention factor, resolution, and asymmetry factors; linearity; detection limit and quantitation limit; and precision) with those of a similar TLC method.

Eastern Pharmacist, July, 113-115 (1999). HPTLC on silica gel with TLC toluene - acetone 1:1. Densitometry at 300 nm. The proposed method is suitable for content uniformity.

J. Liqu. Chroatogr. 23, 609-615 (2000). New procedure HPTLC of oxybenzone (2-hydroxy-4-methoxy-benzophenone) on RP-18 (after prewashing with dichloromethane - methanol 1:1) with methanol - tetrahydrofuran - deionized water 10:7:3 in a paper-lined chamber. Quantitation by densitometry at 300 nm. Precision (RSD) was 2.2% for replicate analysis of four sample solutions of one lotion on one plate and 4.6% for four analyses of one sample solution on different plates. Errors from spiked blanks and standard addition analyses to validate accuracy were 0 and 0.67% for the two blanks and 1.3% for the standard addition validation.

Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 165-171. HPTLC of, xylometazoline hydrochloride and methyl p-hydroxybenzoate on silica gel with ethyl acetate - acetonitrile - diethylamine - water 3:3:1:1. Densitometry at 240 nm in reflectance/absorbance mode. Validation parameters, such as linearity, precision, accuracy and LOQ (limit of quantification) were determined and found to be satisfactory. Simple and reliable method.

J. Planar Chromatogr. 14, 430-434 (2001). TLC of cetirizine dihydrochloride, antazolini hydrochloridum, doxylamine succinate, ketotifene fumarate, chloropyramine hydrochloride on silica gel and aluminium oxide with a variety of mobile phases in horizontal chambers. The best separation selectivity and retention differences for the drugs on the two types of plate were obtained with chloroform - ethyl acetate 1:1, ethyl acetate, and butan-2-one - toluene 7:3. Visualization under 254 nm and by reaction with different reagents. The best sensitivity - 0.06 µg for cetirizine, 0.1 µg for chloropyramine, and 0.2 µg for antazoline, doxylamine, and ketotifene was obtained by spraying with potassium iodoplatinate reagent. Also video densitometry. New simple and rapid method.

Indian Drugs 39 (11), 616-617 (2002). HPTLC on silica gel with toluene - ethyl acetate 93:7. Detection with vanillin-sulfuric acid reagent. The chromatogram showed 9 spots, the spot with RF 0.55 corresponded to eugenol, the major component. Determination also with GC-MS. The results by both methods were comparable.

J. Planar Chromatogr. 15, 414-417 (2002). HPTLC of antimycotics (bifonazole, clotrimazole, fenticonazole, fluconazole, ketoconazole, miconazole, metronidazole, itraconazole) on silica gel with acetone - n-hexane, methanol - toluene, and ethyl methyl ketone - toluene in sandwich chambers, the concentration of organic modifier was varied from 10% to 90%. Detection by exposure to iodine vapor.