Chinese J. of Henan Univ. (Med Sci.) 32 (3), 184-186 (2013). Mingmu Keli granule is a traditional Chinese compound herbal medicine prescribed clinically to treat impaired vision, red eye and eye dryness, macular degeneration, etc. For quality control, TLC 1) for Rhizoma Atractylodis, on silica gel with petroleum ether (60-90 °C) – ethyl acetate 20:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – water 1:10 and heating mildly until the zones are visible in daylight; 2) for Citrus maxima (Burm.) Merr. cv. Tomentosa, on silica gel first over 3 cm with ethyl acetate – methanol – water 100:17:13, then over 8 cm with toluene – ethyl acetate – glacial acetic acid – water 40:20:3:2, detection under UV 366 nm after spraying with 3 % aluminum trichloride in ethanol; 3) for Saposhnikovia divaricata (Trucz.) Schischk., on silica gel (with fluorescence indicator F254 nm) with chloroform – methanol 8:3, detection under UV 254 nm. Quantification of paeoniflorin by HPLC.

J. Jiangxi Univ. Trad. Chinese Med. 26 (2), 55-58 (2014). Xiangshao Shugan Koufuye oral liquid is a TCM preparation for the treatment of chronic gastritis, gastric ulcer, cholecystitis, etc. For quality control, TLC on silica gel (1) for Cyperus rotundus with cyclohexane – ethyl acetate – isopropanol – formic acid 70:30:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 3 min and evaluation at UV 366 nm; (2) for Paeonia lactiflora Pall. and paeoniflorin with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:4 and heating until the zones are visible; (3) for Radix Bupleuri with butanol – ethyl acetate – 10 % ammonia 4:1:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible and at UV 366 nm. Quantitation of paeoniflorin by HPLC.

Strait Pharm. J. 1, 53-55 (2014). Tiaojing Huoxue Jiaonang capsule is a TCM compound used clinically for the treatment of irregular menstruation, menstrual pain and other gynecological diseases. TLC on silica gel (1) for Salvia miltiorrhiza Bunge and protocatechuic aldehyde with dichloromethane – acetone – formic acid 60:5:2, detection by spraying with 5 % ferrum trichloride in ethanol and viewing under white light; (2) for Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey. with petroleum ether (60-90 ˚C) – ethyl acetate 1:1, detection under white light after spraying with 2,4-dinitrophenylhydrazine – ethanol – hydrochloric acid 1:100:10. Quantitative determination of paeoniflorin by HPLC. Applying to real life samples indicated that the method was simple, specific, accurate, reproducible, robust and able to be the better alternative for the purpose to that specified in the Chinese Pharmacopoeia 2010.

J. Liq. Chromatogr. Relat. Technol. 38, 1392-1406 (2015). HPTLC of Cordyceps sinensis and Ganoderma lucidum on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - methanol 10:10:1:1:2. Qualitative identification at UV 254 nm and UV 366 nm. Hyphenation of electronspray ionization/mass spectrometry with HPTLC facilitated fast and convenient profiling of the metabolites in the extracts. For the chemometric analysis, raw and column data matrices were constructed using hRF datasets.

Chinese J. Pharm. Anal. (3), 547-553 (2014). Rhizoma Cimicifugae is a medicinal herb and one of the main ingredients of TCM preparations for the treatment of measles, sore mouth and throat, rashes, chronic diarrhea etc. For quality control, TLC of the extracts of the drug sample on silica gel with the lower phase of chloroform – methanol – water below 10 °C, detection under UV 366 nm revealed 9 pale blue zones. Detection by spraying with a freshly prepared solution of vanillin – sulfuric acid – acetic acid 1:10:1000 followed by heating at 105 ˚C until the zones are visible under UV 366 nm, this detection mode revealed 16 zones of different color. The resulting images were converted to digital profiles to carry out similarity and two-dimensional clustering analysis. The main components were identified using QTofMS with cimifugin, prim-O-glucosylcimifugin, 27-deoxyactein, and ferulic acid as references. Based on the fingerprints the genuine drugs were easily differentiated from adulterant drugs.

J. of China Pharm. 23 (2), 48-51 (2014). Qingbu Tongluo Wan pill is a herbal TCM preparation for the treatment of acute and chronic brucellosis. For quality control, TLC on silica gel (1) for Fructus aurantii, with chloroform – formic acid – water 13:6:2, detection by spraying with 3 % aluminium chloride in ethanol and heating at 105 ºC, detection under UV 366 nm; (2) for Salvia miltiorrhiza Bge., with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC, evaluation in daylight; (3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposing to iodine vapors for 3 min, evaluation under UV 365 nm; (4) for Radix paeoniae rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zonesare visible in daylight. Quantification of salvianolic acid B by HPLC.

Planta Medica 82 (15), 1368-1373 (2016). The incubation mixture of dihydroergotamine with rat liver microsomes was dissolved in methanol – chloroform – ethyl acetate 1:1:1. Preparative TLC on silica gel with methanol – chloroform 1:9. Two zones (hRF 21 and 35) corresponding to produced metabolites were scratched off the plate, separated from the silica with methanol – ethyl acetate 1:1 and, after purification on cyclodextrin, identified through LC-MS-MS and proton-NMR as 5-hydroxyl-dihydroergotamine and 11-hydroxyl-dihydroergotamine, respectively.

Planta Medica 83(01/02), 135-142 (2017). Preparative TLC on silica gel for the purification of recrystallized farnesiferol B (hRF 50; with petroleum ether – ethyl acetate 8:1) and sinkianol B (hRF 40; with chloroform – methanol 15:1) isolated through column chromatography from petroleum ether – dichloromethane fractions of the chloroform extract of Ferula sinkiangensis gum resin.