60th Indian Pharmaceutical Congress PA-220 (2008). HPTLC of domperidone and esomeprazole on silica gel with chloroform - methanol 9:1 with chamber saturation for 30 min. The hRf value was 25 for domperidone and 46 for esomeprazole. Quantitative determination by absorbance measurement at 295 nm. The method was linear in the range of 60-300 ng/spot for domperidone and 80-400 ng/spot for esomeprazole.

J. Chromatogr. B 823 (2), 152-157 (2005). HPTLC of lamotrigine (extracted from serum by ethyl acetate) on silica with toluene - acetone - ammonia 14:6:1. Densitometric measurement at 312 nm, hRf of lamotrigine at 54. The analytical method has excellent linearity (r = 0.998) in the range of 20–300 ng/spot. This assay range is adequate for analyzing human serum, as it corresponds to lamotrigine concentrations measured in human serum from epileptic patients. The method was validated for sensitivity, selectivity, extraction efficiency, accuracy and intra and inter-day reproducibility. The limit of detection and limit of quantification were found to be 6.4 and 10.2 ng, respectively. Good accuracy and high precision (CV) is reported, i.e. in the range of 92.1-97.1 % and 0.5-2.6 % respectively. The method was applied for determination of serum lamotrigine levels in epileptic patients and in pharmacokinetic study of lamotrigine administered orally to rabbits.

Abstract No. F-260, 61st IPC (2009). HPTLC of cefixime and cloxacillin on silica gel, prewashed with methanol, with n-butanol - methanol - water - formic acid 80:60:40:3. The hRf values were 28 and 45 for cefixime and cloxacillin, respectively. Quantitative determination by absorbance measurement at 293 nm for cefixime and 343 nm for cloxacillin. The linearity range was 150-600 ng/band for both compounds.

Abstract No. F-253, 61st IPC (2009). HPTLC of tenofovir and emtricitabine on silica gel with toluene - methanol - ethyl acetate - acetic acid 40:20:50:1. Quantitative determination by absorbance measurement at 273 nm. The hRf value was 52 for tenofovir and 40 for emtricitabine. Linearity of tenofovir and emtricitabine was in the range of 120-600 ng/spot and 80-560 ng/spot, respectively. The recovery was 99.9 and 99.5 % for tenofovir and emtricitabine, respectively.

J. Planar Chromatogr. 22, 277-281 (2009). HPTLC of racecadotril (2-{2(acetylsulfanylmethyl)-3-phenylpropanoyl}amino acetic acid benzyl ester) and its degradation products in the bulk drug and in a pharmaceutical formulation on silica gel with n-hexane - ethyl acetate 7:3 in a twin trough chamber saturated for 30 min. Quantitative determination by absorbance measurement at 230 nm. The limit of detection and quantification was 50 and 100 ng/band, respectively.

Abstract No. F-256, 61st IPC (2009). HPTLC of cefixime trihydrate and erdosteine in combined capsule dosage form on silica gel with ethyl acetate - acetone - methanol - water 15:5:5:3. Quantitative determination by absorbance measurement at 235 nm. The calibration curve was linear between 100 and 500 ng/band for cefixime and 150 to 750 ng/band for erdosteine. The limit of detection and quantification for cefixime was 0.37 µg/mL and 1.14 µg/mL, respectively and for erdosteine 0.33 µg/mL and 1.04 µg/mL, respectively.

J. Planar Chromatogr. 23, 129-133 (2010). TLC of glicazide and glipizide on RP18 silica gel with 60 % acetonitrile in pH 2.3 phosphate buffer in an unsaturated horizontal chambers at room temperature. Detection and quantitative determination by absorbance measurement at 215 nm. Linearity was in the range of 0.8-1.8 µg/zone for both drugs and the correlation coefficients r were 0.998 for gliazide (hRf 38) and 0.993 for glipizide (hRf 51). LOD and LOQ were 50 and 200 ng/zone, respectively, for glicazide and 60 and 300 ng/zone for glipizide.

Abstract No. F-234 61st IPC (2009). A stability-indicating HPTLC method is reported for estimation of diacerein in the presence of its degradation products. HPTLC on silica gel with toluene - ethyl acetate - formic acid 50:30:1. The hRf value of diacerein was 39. Densitometric analysis at 254 nm. The method was found to be linear in the range of 100-600 ng/band. The method was validated regarding stability. The sample was subjected to different stress conditions (acid/base hydrolysis, oxidation, photolysis, thermal) and showed extensive degradation in alkaline medium and mild degradation under acidic and oxidative conditions. The method allowed separation of diacerein from different degradation products.