J. Planar Chromatogr. 16, 298-302 (2003). HPTLC of rutin and quercetin on silica gel, pre-washed with methanol, with n-butanol - acetic acid - water 36:5:5 and toluene - chloroform - acetone - acetic acid 4:4:4:1, respectively. Visualization and quantitation by densitometry at 366 nm. The method was validated using calibration standards of rutin trihydrate and quercetin regarding specificity, limits of detection and quantitation, precision, accuracy and linearity.

CBS 91, 9-11 (2003). HPTLC on silica gel with ethyl acetate - 2-propanol - water 13:5:2 over 65 mm with chamber saturation. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 5-10 min. Visual evaluation in white light and at 254 nm.

Planta med. 70, 54-58 (2004). Analytical and preparative TLC of diospongin A, B, and syringaresinol on silica gel and the monoacetonide and diacetonide of diospongin C, piperitol, and sesaminone on RP-18 with methanol - water 5:2. Analytical TLC of bis(4-N,N-dimethylaminobenzoate) on silica gel using chloroform - methanol 20:1. Detection under UV light at 254 nm.

J. Planar Chromatogr. 18, 319-322 (2005). HPTLC of emodin and phenolic acids (protocatechuic, homoprotocatechuic, caffeic, syringic, vanillic, ferulic, p-hydroxyphenylacetic, alpha-resorcylic, p-coumaric, gallic and ellagic acid) on silica gel in horizontal chambers with toluene - dichloromethane - ethyl acetate 4:4:1. Also two-dimensional TLC of phenolic acids on cellulose with benzene - methanol - acetic acid - acetonitrile 16:2:1:1 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. After drying the chromatograms were observed under UV light at 254 nm before and after treatment with ammonia vapor. Derivatization was performed by spraying with either diazotized sulfanilic acid in 20 % sodium carbonate solution or with 2 % aqueous iron(III) chloride. Detection limits between 10 and 64 ng. Videodocumentation and quantitation by densitometry.

J. AOAC Int. 88, 1562-1567 (2005). HPTLC of beta-asarone (cis-2,4,5-trimethoxy-1-propenylbenzene) and alpha-asarone in Calamus rhizome on caffeine-impregnated silica gel (prepared by immmersion of conventional silica gel plates into a solution of 80 g/L caffeine in dichloromethane for 1 s followed by drying at room temperature for 5 min, then heating at 80 °C for 5 min) with toluene - ethyl acetate 93:7. Quantitative determination by absorbance measurement at 313 nm. The method was validated in terms of stability of sample during chromatography, specificity for beta-asarone, linearity (25-300 ng, including samples containing 0.05-0.7 % beta-asarone), accuracy and precision. Recovery was 100.0-100.8 %, limit of detection 6.4 ng, and limit of quantitation 12.7 ng. The method allows proper identification of Calami rhizoma raw material, and the specific, accurate, and precise quantification of beta-asarone and alpha-asarone

II. Quantitative TLC determination of digoxin and other glycosides on pretreated digitalis lanata extracts. Acta pharmac. Hungarica 52, 133-140 (1982). TLC of digoxin, lanatoside C on silica with dichloromethane - methanol - water 170:27:3 or 80:18:2. Detection with xanthydrol reagent (0.1 g xanthydrol in 96 ml acetic acid + 3 mL H2SO4 + 1 ml HCl). Photometry after elution at 530 nm.

Planta Med. 71, 54-58 (2005). Preparative TLC of microphyllaquinone and (1aS*,1bS*,7aS*,8aS*)-4,5-dimethoxy-1a,7a-dimethyl-1,1a,1b,2,7,7a,8,8a-octahydrocyclopropa[3,4]cyclopenta[1,2-b]naphthalene-3,6-dione on silica gel with hexane - ethyl acetate 7:3 and dichloromethane - chloroform 7:3. Detection under UV light at 250 nm and by spraying with a solution of vanillin - perchloric acid - ethanol, followed by heating at 100 °C for 5 min.

LXXXII. 5, 5-disubstituted barbituric acids and their analogues. Coll. czechoslov. chem. comm. 48, 137-143 (1983). TLC of 5, 5-disubstituted barbituric acids on silica with a) chloroform - methanol -25 % NH3 4:4:2, b) propanol -24 % NH3 7:1:2. Detection by UV.