collected from different phyto-geographical zones of India. J. Liq. Chromatogr. Relat. Technol. 36, 1810-1821 (2013). HPTLC of protodioscin (1) and prototribestin (2) in fruits of Tribulus terrestris on silica gel with n-butanol - glacial acetic acid - water 40:3:10. Quantitative determination by absorbance measurement at 366 nm. The hRf values of (1) and (2) were 41 and 39, respectively. Linearity was in the range of 100-1000 ng/zone for both (1) and (2). LOD and LOQ were 40 and 100 ng/zone for (1) and 35 and 100 ng/zone for (2). Intermediate precision was below 1.0 %. Recovery ranged 98.0-103.0 % for (1) and 99.1-101.9 % for (2).

J. Gansu Coll. of Trad. Chinese Med. 29 (6), 19-22 (2012). Yanyan tablets are a herbal TCM for treating chronic pharyngitis. For quality control, TLC on silica gel 1) for Radix Stemonae, with petroleum ether (60-90 °C) – methanol – ethyl acetate – ethylenediamine 20:20:40:1, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and viewing in daylight; 2) for Radix Ophiopogonis and flowers of common coltsfoot, with petroleum ether (60-90 °C) – acetone 6:1, detection under UV 254 nm. Quantification of rutin by HPLC.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (1), 116-117 (2011). Notopterygium root is the dried root of Notopterygium itwisum Ting ex H. T. Chang and Notopterygium forbesii Boiss and is used to relieve rheumatic pains. Development of a method for qualitative and quantitative determination of nodakenin, one of the key active components in the crude drugs for quality control. TLC of the extracts of the drugs on silica gel with chloroform – methanol 4:1, detection at UV 366 nm. Quantification of nodakenin was done by HPLC.

J. Sep. Sci. 36, 2602-2610 (2013). HPTLC of betulinic acid (1), betulinic acid-3-acetate (2), 3-acetylbetulinaldehyde (3), oleanolic acid-3-acetate (4), 3-beta-hydroxy-28,19-beta-olenolide (5), and beta-sitosterol (6) in the bark of Platanus orientalis on silica gel with n-hexane - toluene - acetone 12:7:2. Detection by dipping into a ceric ammonium sulfate reagent, followed by heating at 105-110 ºC for 5-10 min. Quantitative determination by absorbance measurement at 420 nm for (1), at 550 nm for (2, 3) and at 500 nm for (4–6). The hRf values for compounds (1) to (6) were 16, 42, 85, 37, 25 and 29, respectively. Linearity was in the range of 100-600 ng/zone for (1) to (6). LOD and LOQ were 60 and 100 ng/zone for (1), 80 and 100 ng/zone for (2), 70 and 90 ng/zone for (3), 40 and 80 ng/zone for (4), 100 and 100 ng/zone for (5) and 60 and 90 ng/zone for (6), respectively. Recoveries were in the range of 95-99 % for all the analytes. Intermediate intra- and inter-day precision was below 2 % (n=3).

in Shanhaidanpian tablets) (Chinese). J. of China Pharm. 26 (9), 997-999 (2012). Shanhaidanpian tablets are a herbal TCM preparation for the treatment of poor blood flow, blood stasis and chest pain syndrome. For quality control, TLC on silica gel with toluene – ethyl acetate – formic acid 20:2:1, detection by exposure to vapors until the zones are visible in daylight. Quantitative determination of 2,3,5,4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC.

Chinese J. of Med. Guide 10 (20), 99-102 (2013). Fufang Xiaoji Zhike Milian Gao ointment is herbal TCM preparation for clearing the body heat caused by inflammation, resolving phlegm and relieving asthma. For quality control, TLC on silica gel 1) for Pericarpium Citri Reticulatae and the standard hesperidin, first over 3 cm with ethyl acetate – methanol – water 99:17:13 and thenover 8 cm with toluene – ethyl acetate – formic acid – water 95:45:5:3, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Schisandra Chinensis and the standard schizandrin A, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; 3) for Crataegus pinnatifida and the standard ursolic acid, with toluene – ethyl acetate – formic acid – water 39:8:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 80 °C, detection under UV 366 nm. Quantification of tenuifolin by HPLC.

J. Planar Chromatogr. 27, 267-273 (2014). HPTLC of kaempferol (1), rutin (2), and quercetin (3) in marketed formulations containing_x000D_ Phyllanthus niruri (Bhuiamla) and Emblica officinalis (Amla) on silica gel with chloroform - methanol - formic acid 82:15:10. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (3) were 69, 53 and 12. Linearity was in the range of 21-840 ng/zone for (1), 56-1008 ng/zone for (2) and 60-1800 ng/zone for (3), respectively. The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 21 and 63 ng/zone for (1), 56 and 168 ng/zone for (2) and 300 and 600 ng/zone for (3), respectively. Recoveries for (1) to (3) were obtained in the range of 81.8-119.0 %.

J. Liq. Chromatogr. Relat. Technol. 37, 1568-1582 (2014). HPTLC of artesunate (1) and amodiaquine (2) on silica gel with acetonitrile – water – ammonia 40:7:2. Quantitative determination by absorbance measurement at 366 nm. The hRf values for (1) and (2) were 44 and 50, respectively. Linearity was in the range of 100-600 ng/zone for (1) and 50-300 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 % (n=9). The LOD and LOQ were 25 and 60 ng/zone for (1) and 12 and 30 ng/zone for (2), respectively. Recovery was 98.1-99.9 % for (1) and 99.3-100.9 % for (2).