J. Ethnopharmacol. 124, 450-456 (2010). HPTLC fingerprinting of Eclipta alba on silica gel with chloroform - ethanol - water 35:10:2. Densitometric evaluation at 254 nm. Major compounds identified were coumestants and wedelolactone, with hair growth promoting activity.

Abstract No. C-161, 61st IPC (2009). An HPTLC method is reported for estimation of andrographolides bitter principles in Andrographis paniculata, popularly known as kalmegh. HPTLC of methanolic and water extracts on silica gel with chloroform - methanol 7:1 in a saturated twin trough chamber. Quantitative evaluation by absorbance measurement at 231 nm. The method was found to be linear in the range of 1-5 µg/band. Both extracts were found to contain andrographolides. Maximum yields of andrographolides were observed in extracts prepared by refluxing.

International Seminar on Herbal Drug Research, PN-051 (2009). HPTLC of four different extracts of rhizomes of Cyperus rotundus on silica gel with chloroform - benzene 1:1. Evaluation under 254 nm. Detection by treatment with ethanolic potassium hydroxide and evaluation of the fingerprint under daylight.

Journal of Pharmacy Research 3(5), 1107-1109 (2010). HPTLC of mangiferin in Salacia chinesis (Hippocrateaceae) on silica gel with ethyl acetate - methanol 2:3 at 25 °C with chamber saturation for 30 min. Densitometric evaluation at 254 nm. Derivatization by spraying with acetic anhydride-sulfuric acid-ethanol reagent, followed by heating at 110 °C for 2 min. The method was linear in the range of 10-200 ng/band. The plant was found to contain 1.54 % of mangiferin.

Chinese J. Mod. Drug Appl. 4(15), 16-17 (2010). TLC on silica gel 1) with n-butanol – glacial acetic acid – water 4:1:5 for Xanthium sibiricum Patr., detection by exposure to iodine vapors; 2) with trichloromethane – diethyl ether 5:1 for Flos magnoliae, detection by spraying with 10 % sulfuric acid in ethanol and heating at 90 °C until the zones were visualized; 3) with n-butanol – ethyl acetate 17:3 for herba Menthae, detection by spraying with vanillin reagent and heating at 105 °C until the zones were visible; 4) with petroleum ether (30-90 °C) – ethyl acetate 17:3 for Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., detection under UV 366 nm.

Planta Med. 75, 711-718 (2009). For many decades, planar chromatography has been used for the analysis of plants, in particular today in its most advanced form of HPTLC. The technique is e. g. used for the identification of medicinal plants and dietary supplements, and for the detection of adulteration and quantitative determination of marker substances. Reliable qualitative and quantitative results can be achieved based on suitable instrumentation and adequate methodological concepts. The manageability of the entire planar chromatographic process has improved. Integration of biological detection systems as well as hyphenation to mass spectroscopy has widened the applicability of planar chromatography as an important analytical technique. The introduction is followed by explanation of HPTLC, use of HPTLC in plant analysis, limitations, applications (identification, detection of adulteration and quantitation), and instrumentation (chromatogram development, documentation, detection and evaluation).

Tianjin J. of Trad. Chinese Med. & Pharm. 28 (2), 164-166 (2011). TLC of the extracts of the traditional Mongolian medicine on silica gel 1) for artificial cow-bezoar, with isooctane - ethyl acetate - glacial acetic acid 6:3:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected under UV 365 nm, identification by comparison with the fingerprint of the individual drug components and cholic acid as reference; 2) for Coptidis rhizoma, with benzene - ethyl acetate - isopropanol - methanol - water 20:10:5:5:1, detection under UV 365 nm after exposure to ammonia vapour, identification by comparison of the fingerprint with the individual drug components and berberine hydrochloride as reference; 3) for light yellow Sophora root, with ethyl acetate - propanone - benzene - ammonia water 20:15:10:1, detection by spraying with 5 % potassium iodobismuthate solution, identification by comparison of the fingerprint with the individual drug components and with matrine as reference.

J. Planar Chromatogr. 24, 66-71 (2011). HPTLC of extracts of Stresroak premix and gallic acid (1), mangiferin (2), and withanolide A (3) as standards on silica gel with A) ethyl acetate - formic acid - acetic acid - water 100:11:11:27, for (1) and (2), and B) chloroform - methanol 9:1 for (2) in a twin trough chamber. Quantitative determination by absorbance measurement at 280 nm for (1), 330 nm for (2), and 225 nm for (3). The hRf values of (1), (2) and (3) were 76, 29, and 48, respectively. The average recoveries were 100.4 % (1), 99.3 % (2) and 98.0 % (3). The linear concentration range was 50-150 ppm for (1), and 40-100 ppm for (2) and (3). The LOD, defined as the amount of compound required to produce a signal at least three times the noise level, for gallic acid, mangiferin, and withanolide A was 80, 110, and 200 ng for (1), (2), and (3), respectively. The LOQ was 20, 27, and 38 µg, respectively.