Indian Drugs 41 (6), 354-357 (2004). HPTLC on silica gel with toluene - methanol - acetone - 10 % ammonia 80:40:20:1. Quantitative determination by absorbance measurement at 254 nm. The Rf value of pioglitazone hydrochloride was found to lie between 0.49-0.55. The linear dynamic response was found to be 2.0-4.0 µg/spot for pioglitazone hydrochloride. The results of the analysis have been validated statistically and by recovery studies. A simple, fast, specific and precise HPTLC method has been developed for the estimation of pioglitazone hydrochloride in its tablet dosage form.

IPC 56th 2004, Abstract No. D-9. HPTLC for the standardization of the alkaloid conessine in several market formulations containing kurchi bark, on silica gel with toluene - methanol - chloroform 1:2:1. Detection by spraying with Dragendorff’s reagent. Densitometric evaluation at 460 nm. The method was validated for accuracy, precision, linearity range, specificity, LOD, LOQ and found suitable for routine analysis of herbal formulations containing Kurchi as main ingredient.

Part I. J. Planar Chromatogr. 17, 275-279 (2004). HPTLC of nicotinic acid and selected derivatives (methyl nicotinate, ethyl nicotinate, isopropyl nicotinate, butyl nicotinate, hexyl nicotinate, benzyl nicotinate, nicotinamide, N-methyl nicotinamide) on RP-18 with methanol - water in different volume proportions after chamber saturation for 30 min. Detection under UV light at 254 nm. Investigation of the lipophilicity by TLC and use of the data for quantitative structure-activity relationships.

J. AOAC Int. 87, 827-833 (2004). TLC of trimetazidine dihydrochloride and degradation products (e. g. piperazine and 2,3,4-trimethoxymethyl benzene) on silica gel with methanol - ammonia 200:3. Detection under UV light at 254 nm and densitometry at 215 nm. The method was applicable over a concentration range of 2.00-9.00 µg/spot with a mean percentage accuracy of 99.86 +/- 0.92.

J. Planar Chromatogr. 18, 450-454 (2005). TLC of twenty-seven 2-benzylsulfanybenzothiazole derivatives on silanized silica gel in a pre-equilibrated normal chamber with 0.05 M phosphate buffer (pH 7.4 or 3.0) and methanol as organic modifier. Detection under UV light at 254 nm.

Investigations on RP 18 WF254 plates. Part II. J. Planar Chromatogr. 18, 300-304 (2005). HPTLC of nicotinic acid, methyl nicotinate, ethyl nicotinate, isopropyl nicotinate, butyl nicotinate, hexyl nicotinate, benzyl nicotinate, and N-methylnicotinamide on RP-18 W after presaturation of the chamber with methanol - water in different volume proportions. Visualization under UV light at 254 nm.

Indian Drugs 42 (10), 650-653 (2005). A simple rapid, precise and cost-effective HPTLC method has been developed for the determination of ursolic acid in Oscimum sanctum (Tulsi) leaves and its formulations (Tulsi ghan tablets and Tulsi capsules). HPTLC on silica gel with toluene - ethyl acetate - acetic acid 30:3:1. Detection with anisaldehyde in sulphuric acid reagent followed by heating in an oven at 110 °C. Quantitative determination by absorbance measurement at 580 nm. Linearity of the detector response was given in the range of 40 - 280 ng. LOD was 8 ng. The correlation coefficient obtained from linearity was 0.9985. The standard error was 26.511. The mean assay values of ursolic acid wa found to be 3.485 mg/g, 0.553 mg/g and 3.221 mg/g in tulsi ghan tablets, tulsi capsule and tulsi leaves respectively.

J. Planar Chromatogr. 19, 204-207 (2006). HPTLC of florfenicol on silica gel in a twin-trough chamber with ethyl acetate - n-hexane 4:1. Quantitative determination by absorbance measurement at 223 nm. Linearity range of calibration curve was 20 -80 ng with a correlation coefficient r2 of 0.9987. Limit of detection was 2.55 mg / kg and limit of quantification was 8.50 mg / kg. Recovery was 101.7 % at 50 mg /kg, 85.2 % at 500 mg / kg and 81.9 % at 1500 mg / kg. Precision was evaluated based on intra-laboratory dispersion or repeatability. RSD for 50, 500, and 1500 mg / kg was 2.30, 2.72, and 3.57 % respectively.