J. of China Pharm. 22 (9), 14-16 (2013). Sanyu Xugu Tie plaster is a herbal TCM preparation for the treatment of traumatic injury, contusion, stasis of blood and innominate toxic swelling. For quality control, TLC on silica gel 1) for Radix Cynanchi paniculati and the standard 2'-hydroxy-4'-methoxyacetopheone, with cyclohexane – ethyl acetate 3:1, detection by spraying with 5 % ferric chloride in ethanol and heating until the zones are visible in daylight; 2) for Oleum eucalypti and the standard cineole, with cyclohexane – ethyl acetate 19:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 3) for Herba sarcandrae and the standard isofraxidin, with toluene – ethyl acetate – formic acid 9:4:1, detection under UV 366 nm; 4) for Kusnezoff monkshood root and the standard acetylbenzoylaconine, with cyclohexane – ethyl acetate diethylamine 12:8:1, detection by spraying with 5 % potassium iodobismuthate in water – hydrochloric acid 200:1 and viewing in daylight.

using a polyamide plate with nonaqueous and reversed micellar mobile phases. J. Planar Chromatogr. 26, 463-469 (2013). 2D TLC fingerprint of Helleborus thibetanus Franch on polyamide with chloroform - ethyl acetate - methanol 15:40:22 in the first dimension and isooctane - n-propyl alcohol - water 20:5:1 + 0.28 M sodium dodecyl sulfate in the second dimension, developing distances were 70 mm for both. Detection under UV 366 nm. Nine zones were detected for the identification of Helleborus thibetanus Franch.

Chinese J. of Heilongjiang Med. 26 (3), 382-385 (2013). Gongxueting Keli granules are a herbal TCM preparation for treating women’s asthenia of both the spleen and kidney, and blood stasis due to menstruation and metrorrhagia. For quality control, TLC on silica gel 1) for Eclipta and the standard ecliptasaponin A, with dichloromethane – ethyl acetate – methanol – water 30:40:15:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 2) for Leonurus Artemisia (Laur.) S. Y. Hu F and the standard stachydrine hydrochloride, with acetone – ethanol – hydrochloric acid 10:6:1, detection by heating at 105 °C for 15 min followed by spraying with 10 % sulfuric acid in ethanol and then heating, followed by spraying with 5 % potassium indobismuthate – 1 % ferric chloride in ethanol 10:1, viewing in daylight.

Chinese J. of Ethnomed. & Ethnopharm. 3, 41-43 (2012). Yufukang Jiaonang capsules are a herbal TCM preparation for the treatment of numbness of the tongue, language disadvantage, choke and cough when eating, aphasia, etc. For quality control, identification of the main component crude drug, the root of Salvia miltiorrhiza and the standard tanshinone llA by TLC on silica gel with toluene – ethyl acetate 19:1, detection in daylight.

Chinese J. of Med. Guide 11 (16), 507-509 (2013). Xiaoxumingtang keli granule is a herbal TCM effective for promoting blood circulation and removing blood stasis, prescribed as an assistant drug for the treatment of stroke, hemiplegia，rheumatis, etc. For quality control, TLC 1) for Saposhnikovia divaricata (Trucz.) Schischk. and the standards isoacteoside and 4’-o-beta-glucopyranosyl-5-o-methylvisamminol on silica gel with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection under UV 254 nm; 2) for Panax ginseng C. A. Mey. and the standards ginsenoside Rg1, Re, and Rb1 on silica gel with chloroform – methanol 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight and under UV 366 nm; 3) for Ligusticum chuanxiong Hort. and the standards ligustrazine and ferulic acid on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 4) for Paeonia veitchii and the standard paeoniflorin, on silica gel with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 5) for Ephedra sinica Stapf and the standard (1R,2S)-(-)-ephedrine hydrochloride, on silica gel with chloroform – methanol – concentrated ammonia 40:10:1, detection by spraying with 0.1 % ninhydrin in acetone, heating at 105 °C until the zones are visible in daylight; 6) for Scutellaria baicalensis Georgi and standard baicalin, on plates made of silica gel - sodium acetate – 0.3 % CMC 50:8:150, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation in daylight.

J. Planar Chromatogr. 27, 199-203 (2014). HPTLC of myotonin in QiYi capsules (preparation with astragalus, myotonin, thunder god vine and centipede) on silica gel with petroleum ether - diethyl ether - glacial acetic acid 83:17:1. Detection by spraying with vanillin reagent (1 % in sulfuric acid), followed by heating at 105 ºC for 3 min. Quantitative determination by absorbance measurement at 365 nm. The hRf value for myotonin was 19. The intermediate/interday/intra-day precisions were below 3.4 % (n=5).

Chinese J, Mod. Drug Appl. 7 (14), 1-3 (2013). Tongbianning Pian tablet is a herbal TCM for the treatment of habitual constipation disease. For quality control, TLC on silica gel 1) for Folium Sennae, with chloroform – methanol – ammonia 190:8:1, detection by exposure to ammonia vapors under UV 366 nm; 2) for Pharbitis nil (Linn.) Choisy, with toluene – glacial acetic acid 100:1, detection by spraying with 5 % vanillin in sulfuric acid – ethyl acetate 22:1 and heating mildly until the zones are visible in daylight; 3) for Amomum microcarpum C. F. Liang et D. Fang and Amomum kravanh, with cyclohexane – glacial acetic acid 100:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zones are visible in daylight. Quantification of aloe emodin by HPLC.

Phytochemistry 106, 94-103 (2014). HPTLC of corosolic acid in Lagerstroemia speciosa on silica gel with ethyl acetate - methanol 49:1. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 580 nm. The hRF value of corosolic acid was 47. Linearity was between 20 and 100 ng/zone. The intermediate precisions were below 2 % (n=3).