J. Liq. Chromatogr. Relat. Technol. 34, 579-590 (2011). HPTLC fingerprint of andrographolide (1), kutkoside (2), picroside-I (3), phyllanthin (4), and hypophyllanthin (5) in pharmaceutical formulations containing Andrographis paniculata, Phyllanthus amarus and Picrorhiza kurroa extracts, on silica gel with toluene - ethyl acetate - methanol - formic acid 8:7:2:1. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) - (5) were 58, 13, 21, 73 and 82, respectively. The repeatability (%RSD) of sample application and measurement of peak areas was 0.1 and 0.3 % for (1), 0.04 and 0.14 % for (2), 0.13 and 0.11 % for (3), 0.08 and 0.07 % for (4), and 0.16 and 0.12 % for (5), respectively. Linearity was between 1000-3000 ng/zone for (1), 350-1050 ng/zone for (2) and (3), 175-525 ng/zone for (4), and 50-150 ng/zone for (5). The intermediate/inter-day/intra-day precisions (n = 7) were 0.2 % for (1-3), 0.1 % for (4), and 0.3 % for (5) .

J. Planar Chromatogr. 24, 541-544 (2011). HPTLC of alpha-asarone (alpha-(1,2,4-trimethoxy-5-[(E)-prop-1-enyl]benzene) in rhizome extracts of Acorus calamus on silica gel with toluene - ethyl acetate 8:3 in a twin-trough chamber with saturation for 30 min. Detection and quantitative determination by densitometry at 254 nm. The linearity was in the range of 200-1000 ng/zone with a correlation coefficient of 0.996. The hRf was 71. LOD and LOQ of the standard were 60 and 173 ng/zone, respectively. Robustness was performed at concentration levels of 200, 400, and 800 ng/zone and the %RSD of peak areas was calculated. The %RSD for robustness analysis was less than 2 % in all cases, which indicated that the experimental procedure was in the range of acceptability as there was not much deviation.

J. of Integrated Modern Trad. Chinese & Western Med. 20(13), 1634-1636 (2011). TLC of the extracts of Yinlishuang lotion on silica gel 1) for Phellodendron with chloroform - methanol - ammonia 50:10:1, detection under UV 365 nm; 2) for radix Sophorae flavescentis, with toluene - acetone - methanol 8:3:1, detection by spraying with diluted potassium iodobismuthate; 3) for fructus Cnidii, with toluene - ethyl acetate - n-hexane 3:3:2, detection under UV 365 nm. Identification by comparison of the fingerprint with the standards of the individual drug components.

J. of China Pharm. 20 (9), 19-20 (2011). Shujian capsules are a herbal TCM preparation prescribed clinically to treat shoulder arthritis. For quality control, TLC on silical gel for 1) for Cassia twig, with petroleum ether (60-90 ºC) – ethyl acetate 17:3, detection by spraying with 2 % 2,4-dinitrophenylhydrazine in ethanol and viewing in daylight; 2) for Radix sileris, with chloroform – methanol 4:1, detection at UV 254 nm; 3) for Angelica sinensis and Ligusticum wallichii, with toluene - ethyl acetate – formic acid 40:10:1, detection by spraying with 1 % ferric chloride – 1 % potassium ferricyanide 1:1 and viewing in daylight; 4) for the root of Kudzu vine, with chloroform – methanol – water 28:10:1, detection under UV 366 nm.

J. of China Pharm. 26 (5), 503-504 (2012). E Ling capsule is a herbal TCM for activating blood circulation to dissipate blood stasis, relieving pain, eliminating stagnation, and is prescribed clinically to treat pelvic endometriosis. For quality control, TLC of the extracts of the medicine on silica gel 1) for the root of red-rooted salvia, with toluene – ethyl acetate 19:1, detection by viewing in daylight; 2) for Fructus Aurantii, first over 4 cm with ethyl acetate – methanol – water 100:17:10, then over 8 cm with toluene – ethyl acetate – formic acid – water 20:10:1:1, detection at UV 366 nm after spraying with 1 % aluminium chloride in ethanol; 3) for Rhizoma Corydalis, with n-hexane – chloroform – methanol 15:8:2, detection at UV 366 nm.

J. Planar Chromatogr. 25, 295-300 (2012). HPTLC of vanillin-ß-D-glucoside (1), p-hydroxybenzaldehyde (2), vanillic acid (3), p-hydroxybenzoic acid (4) and vanillin (5) on silica gel with n-hexane - chloroform - methanol 5:36:4 + 1 drop glacial acetic acid as mobile phase. Quantitative determination by absorbance measurement at 254 nm for (3) and (4), 280 nm for (1) and (2) and 313 nm for (5). The hRf values for compounds (1) to (5) were 9, 62, 57, 42 and 77, respectively. Linearity was in the range of 24-121 ng/band for (1), 7-33 ng/band for (2), 20-102 ng/band for (3), 21-106 ng/band for (4) and 14-70 ng/band for (5). Limits of detection and quantification were 8 and 20 ng/band for (1), 2 and 6 ng/band for (2), 14 and 20 ng/band for (3), 6 and 20 ng/band for (4) and 4 and 8 ng/band for (5), respectively. Intermediate/inter-day/intra-day precision was below 1.4 % (n=6). Mean recovery for the compounds was between 96.8 and 101.4 %.

J. Planar Chromatogr. 25, 523-527 (2012). TLC of a mixture of 12 non-steroidal anti-inflammatory drugs on silica gel with ethyl acetate - toluene - methanol - acetic acid 40:40:1:1. Quantitative determination by absorbance measurement at 254 nm. Limits of detection were in the range of 5-500 ng/zone. The method showed comparable results to a validated HPLC method.

J. Liq. Chromatogr. Relat. Technol. 35, 1565-1584 (2012). HPTLC of negundoside (1), ursolic acid (2), eugenol (3), lupeol (4), and beta-sitosterol (5) in the leaves of Vitex Negundo on silica gel with toluene - methanol 9:1. Detection by dipping in anisaldehyde sulfuric acid reagent for approximately 1 min, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 525 nm. The hRf value of compounds (1) to (5) were 47, 61, 56, 54 and 38, and selectivity regarding matrix was given. Linearity was in the range of 200-800 ng/zone for (1), 72-576 ng/zone for (2), 200-1000 ng/zone for (3), 150-900 ng/zone for (4) and 80-480 ng/zone for (5). Limits of detection and quantification were 80 and 200 ng/zone for (1), 18 and 72 ng/zone for (2), 60 and 200 ng/zone for (3), 50 and 100 ng/zone for (4) and 20 and 60 ng/zone for (5), respectively. The method provides acceptable intra-day and inter-day precision for (1) to (5). The average recoveries for compounds (1) to (5) were found to be 99.9, 100.1, 99.7, 100.0, and 99.9 %, respectively.