IPC 56th 2004, Abstract No. GP-5. Simultaneous HPTLC determination of amlodipine and atenolol on silica gel with ethyl acetate - methanol - ammonia 60:40:3. Quantitative determination by scanning at 254 nm. The method was found linear in the range of 0.5 – 5.0 mg/mL amlodipine and 5.0 mg – 50 mg/mL atenolol. Recovery was 98.11 – 101.5 % for both of the compounds. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

J. Planar Chromatogr. 17, 224-228 (2004). TLC of fluvoxamine and moclobemide on silica gel in horizontal chambers with benzene - acetone - ethanol - 25 % ammonia 9:7:2:1. Densitometric detection and quantification were performed at 249 nm and 236 nm, respectively. The range of linearity was 1 - 10 µg per spot; the RSD was less than 2.5 % for densitometry and less than 5.1 % for videodensitometry.

J. Planar Chromatogr. 18, 330-332 (2005). TLC of diazepam, phenobarbitone, chloral hydrate, copper sulfate, sulfadiazine, and sacharin on silica gel in a presaturated chamber with chloroform - acetic acid 9:1, n-hexane - acetone - methanol 16:6:1 and n-hexane - acetone - butanol 24:16:1. Detection by treatment with chlorine followed by spraying with o-tolidine reagent and 1 % phosphomolybdic acid.

J. AOAC Int. 88, 70-79 (2005). HPTLC of haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in 25 selected psychotropic drugs on silica gel with 30 mobile phases, eight of them were selected based on spot location and developing time. Identification and quantification were carried out based on UV densitometric measurements. In addition to retention factors, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Limit of detection ranged from 0.009 to 0.260 µg depending on the wavelength used. A satisfying recovery, ranging from 92.9 to 104.7 %, was achieved for individual constituents.

J. Planar Chromatogr. 18, 336-343 (2005). HPTLC of thirty-three compounds with benzodiazepine properties on silica gel after prewashing first with methanol and then with dichloromethane - methanol 19:1 in a saturated horizontal chamber and three optimized mobile phases: dichloromethane - methanol 19:1, ethyl acetate - cyclohexane - 25 % ammonia 50:40:0.1, and in a third run cyclohexane - acetone - methyl t-butyl ether 3:2:1. Diode-array HPTLC makes it possible to identify all the compounds with high certainty down to a level of 20 ng. An algorithm for spectral recognition which is combined with Rf values from the three separation steps into one fit factor is presented. This set of data is unique for each of the compounds investigated and enables unequivocal identification.

J. Liq. Chrom. & Rel. Technol. 27, 3121-3134 (2004). TLC of six quinazoline derivatives on silica gel with chloroform - ethyl acetate 19:1 and ethyl acetate - acetonitrile 7:13 in a horizontal chamber. Detection under UV light at 254 nm.

Indian J. Pharm. Sci. 67 (3), 351-355 (2005). HPTLC of carbamazepine, phenytoin, and phenobarbitone in tablets on silica gel with toluene - acetone 5:2. Quantification by absorbance measurement at 217 nm. This method was quantitatively evaluated in terms of linearity, accuracy, precision, repeatability and specificity proving its utility in estimation of drug content in tablet dosage form.

J. Planar Chromatogr. 19, 302-306 (2006). HPTLC of pantoprazole sodium sesquihydrate and domperidone on silica gel, prewashed with methanol, at 23 - 27 °C in a pre-saturated twin-trough chamber with methanol - water - ammonium acetate 8:2:1. Quantitative determination by absorbance measurement at 286 nm. The method was validated in accordance with ICH guidelines.