Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      130 023
      Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances
      F. LEONG (Leong Fong), X. HUA (Hua Xue), M. WANG (Wang Mei), T. CHEN (Chen Tongkai), Y. SONG (Song Yuelin), P. TU (Tu Pengfei), X. CHEN (Chen Xiao-Jia)* (*State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China; xiaojiachen@um.edu.mo)

      Chinese Medicine 15, 76 (2020). This review compared the 2020 editions of Chinese (ChP) and European Pharmacopoeas (EuP) in different aspects of quality control of traditional Chinese medicinal plants (73 of which drugs were common to both, but with differences in species or organs for 17 of them). Discussed points included history, identification, plant origin and processing, sample preparation, marker selection, tests and assays, as well as advanced analytical techniques for quality control and for the establishment of comprehensive quality standard. TLC was discussed in relation to its following aspects: purposes, markers/references, techniques and result description.
      (A) The main uses of TLC and HPTLC were (1) chemical-based identification of the plant in a more accurate and precise method than by macroscopic and microscopic observation only, and in a more direct and easily interpretation than HPLC, and allowing the simultaneous analysis of multiple samples in parallel; (2) control of possible adulterants; (3) quantification of active compounds. Both uses (1) and (2) were combined in some EuP monographs: as example were given the roots of Angelica dahurica, A. pubescens, A. sinensis, using TLC for identification of the species and of adulterants from other species (Angelica, Levisticum and Ligusticum).
      (B) In ChP, identification through TLC was in most cases achieved by fingerprint comparison to an official reference extract or herb (herbal reference substance). At the opposite, EuP often indicated analytical markers, irrespective of any pharmacological activity, but chosen only for analytical purposes in TCM identification and quantification. Examples were: aescin and arbutin as analytical markers for TLC identification of Anemarrhena asphodeloides rhizome and Panax notoginseng root.
      For the TLC system suitability assessment tests, ChP used the same intensity markers or active markers that were chosen for the identification or assay; whereas EuP often used other specific references, e.g. isoeugenol and methyleugenol in the case of Ophiopogon japonicus roots.
      (C) For the techniques, conventional separations and chemical derivatizations were used. Hyphenations of TLC to other analytical methods (e.g. MS) were absent. Only one monograph applied an effect-directed analysis directly on TLC chromatogram (free DPPH• radical scavenging assay for TLC identification of Rehmannia glutinosa root, in ChP).
      Sometimes, the TLC methods were different between both reference books for the same species. Example was given for Belamcanda chinensis (=Iris domestica) rhizome: in EuP, development on silica gel with cyclohexane – ethyl acetate – acetic acid 20:80:1, detection under UV 254 nm, comparison to standards coumarin and irisflorentin; whereas in ChP, development on polyamide layer with chloroform – butanone – methanol (3:1:1), detection under UV 365nm after derivatization with aluminium chloride, comparison to a reference rhizome powder.
       (D) Finally, the results in ChP were described as a text stating the similarity of sample profile with the profile of the chosen reference, whereas the results in EuP were described with a schematic box indicating the positions of bands of interest.

      Classification: 1, 2a, 32e
      130 056
      Ecofriendly appraisal of stability-indicating high-performance chromatographic assay of canagliflozin and metformin with their toxic impurities; in silico toxicity prediction
      R. EMAM, A. EMAM* (*Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62514, Beni-Suef, Egypt, aml17484@gmail.com)

      J. Sep. Sci. 202200754 (2022). HPTLC of canagliflozin (1) and its major degradation product (2); metformin (3) and its major degradation product cyanoguanidine (4), and metformin toxic impurity melamine (5) on silica gel with acetone - ethyl acetate - acetic acid 40:10:1. Quantitative determination by absorbance measurement at 205 nm. The hRF values for (1) to (5) were 21, 35, 47, 71 and 83, respectively. Linearity was between 200 and 3000 ng/zone for (1), 50 and 3000 ng/zone for (2), 50 and 2500 ng/zone for (3), 20 and 2500 ng/zone for (4) and (5). Inter-day and intra-day precisions were below 2 % (n=9). The LOD and LOQ were 17 and 51 ng/zone for (2), 6 and 19 ng/zone for (4) and 3 and 11 ng/zone for (5). Mean recovery was 101.8 % for (1), 100.9 % for (2), 99.4 % for (3), 102.0 % for (4) and 98.4 % for (5). 

      Classification: 32a
      130 057
      Sensitive and selective bioscreening of the most commonly used coronavirus disease drug, favipiravir, and its co-administered therapeutic, meropenem, in human plasma
      R. ABDELFATAH, E. ABDELALEEM, E. ABDELMOMEN*, R. ABDELMOETY, A. EMAM (*Faculty of Pharmacy, Nahda University (NUB), New Beni-Suef City, Egypt, dresraahossam90@gmail.com)

      J. Sep. Sci. 45, 3800-3810 (2022). HPTLC of favipiravir (1) and meropenem (2) in human plasma on silica gel with ethyl acetate - methanol - water - formic acid 50:40:15:3. Quantitative determination by absorbance measurement at 300 nm for (1) and (2). The hRF values for (1) and (2) were 34 and 10, respectively. Linearity was between 0.1 and 20 µg/mL for (1) and 10 and 60 µg/mL for (2). Inter-day and intra-day precisions were below 5 % (n=3). The LOQ were 0.1 and 10 µg/mL for (1) and (2), respectively. Mean recovery was 99.7 % for (1) and 98.0 % for (2).

      Classification: 32f
      130 059
      Evaluation of the efficacy and safety of Unani formulations in Pityriasis versicolor: A randomized controlled trial
      B. ISMAIL, M. NAWAB*, S. FATIMA, A. MINHAJUDDIN, M. NAIKODI (*Department of Moalajat (Medicine), National Research Institute of Unani Medicine for Skin Disorders, Opp. ESI Hospital, Eragadda, Hyderabad, 500038, India, ccrumnawab@gmail.com)

      J. Ethnopharmacol. 289, 115035 (2022). HPTLC of Unani pharmacopoeial preparations Itrifal Hakim Ali (1) and Habb-e-Kalaf (2) on silica gel with toluene - ethyl acetate 9:1 for (1) and toluene - ethyl acetate 4:1. Detection under UV light at 254 and 366 nm. 

      Classification: 32e
      130 060
      Genus Rauvolfia: A review of its ethnopharmacology, phytochemistry, quality control/quality assurance, pharmacological activities and clinical evidence
      S. KUMAR*, D. KUMARI, B. SINGH (*Department of Chemistry, Ma. Kanshiram Government Degree College, Ninowa, Farrukhabad, 209602, India, sunilchem1986@gmail.com)

      J. Ethnopharmacol. 295, 115327 (2022). Review of modern applications for the analysis of Rauvolfia species, including traditional uses, phytochemistry, quality control, pharmacological properties, as well as clinical evidence that may be useful in the drug discovery process. The paper described qualitative and quantitative methods, including HPTLC methods for the analysis of targeted and non-targeted compounds in different extracts of plant parts of Rauvolfia species.

       

      Classification: 32e
      130 067
      High-performance thin-layer chromatography coupled attenuated total reflectance-Fourier-transform infrared and NMR spectroscopy-based identification of α-amylase inhibitor from the aerial part of Asparagus racemosus Willd
      P. DAS, G. ASHRAF, T. BAISHYA, T. DUA, P. PAUL, G. NANDI, R. SAHU* (*Ranabir Sahu, Department of Pharmaceutical Technology, University of North Bengal, Darjeeling 734013, India, ranabirsahu@nbu.ac.in)

      Phytochem. Anal. 33, 1018-1027 (2022). HPTLC of an α-amylase inhibitor from the aerial part of Asparagus racemosus Willd on silica gel with n-hexane - ethyl acetate - acetic acid 20:10:1. Detection of free-radicals scavenging active compounds by spraying with 0.2 % DPPH• solution in methanol, followed by incubation for 30 min in the dark at room temperature. The antioxidant activity was measured by adding the area of the bright yellow zones and expressing it to gallic acid equivalents (GAEs), using gallic acid (1–15 μg/μL) to construct a calibration curve as the model analyte. Biochemical analysis by dipping into freshly prepared α-amylase solution, followed by incubation at 37 °C for 40 min in a humid chamber. After incubation, the plate was immersed in the substrate solution (2 % starch solution), followed by incubation for another 15 min to allow the enzyme-substrate reaction to occur. Detection by spraying with Gram's iodine solution. By comparing the area of blue bands obtained by samples and standard acarbose the inhibitory activity was measured. Linearity was in the range of 1-7 μg/zone for acarbose and 1-15 μg/zone for gallic acid. The LOD and LOQ were 3 and 9 μg for both acarbose and gallic acid. Further analysis by total reflectance-Fourier-transform infrared (ATR-FTIR) and nuclear magnetic resonance (NMR) spectroscopy.

      Classification: 32e
      130 079
      Recent advances in exhaled breath sample preparation technologies for drug of abuse detection
      F. XU (Wu Fei), J. ZHOU (Zhou Jiedan), H. YANG (Yang Hai), L. CHEN (Chen Linzhou), J. ZHONG (Zhong Jinjian), Y. PENG (Peng Yihong), K. WU (Wu Ke), Y. WANG (Wang Yukai)*, H. FAN (Fan Huajun), X. YANG (Yang Xiangliang), Y. ZHAO (Zhao Yuliang) (*The GBA National Institute for Nanotechnology Innovation, Guangzhou, 510535, China, gdgawhk@163.com)

      Trends Anal. Chem. 157, 116828 (2022). Review of the analysis of drugs of abuse in exhaled breath. The paper described sampling devices, sample pretreatment techniques and the application of chromatographic techniques, including TLC for the analysis of drugs of abuse.

      Keywords: HPTLC review toxicology
      Classification: 32d
      130 024
      A multivariate analysis on the comparison of raw notoginseng (Sanqi) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography
      X. ZHOU, V. RAZMOVSKI-NAUMOVSKI, K. CHAN* (National Institute of Complementary Medicine, University of Western Sydney, Penrith, and Faculty of Pharmacy, The University of Sydney, Sydney, Australia; *k.chan@uws.edu.au)

      Chinese Medicine 10, 13 (2015). Samples were root and rhizome extracts of Panax notoginseng (Araliaceae), either raw or in the form of commercial granules. Standards were ginsenosides Rg1, Rb1, Rd, Re and Rg2, notoginsenoside NR1. TLC on silica gel with chloroform – ethyl acetate – methanol – water 15:40:22:9, followed by 10 min air drying. Derivatization for ginsenosides by immersion into sulfuric acid (10 % in ice cold methanol), followed by 10 min air drying and 5 min heating at 100 °C. Quantification by densitometric fluorescence measurement (deuterium and tungstene lamp, 366 nm). For each standard the linear range was 0.05-1 mg/mL (LOQ comprised between 38 and 431 µg/µL). As NR1 and Re (ratio ca. 2:1) had almost the same hRF, they were quantified together as one substance. Multivariate analysis through hierarchical (HCA) and principal component analyses (PCA) was used to order the samples into two clusters, according to the analyte concentrations, the raw plant extracts being richer than most of the commercial products. This TLC method was compared to quantification through UPLC-PDA (Ultra-performance liquid chromatography with photo diode array), which was more sensitive (LOQ between 10 and 49 µg/µL) but did not allow the separation between Rg1 and Re (ratio ca. 6:1).

      Classification: 14, 32e
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