Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 098
      Ultra-Performance Liquid Chromatographic and densitometric methods for sensitive determination of xipamide and triamterene in pure and pharmaceutical dosage forms
      N. FARES, H. EL FIKY*, A. BADAWEY, M. EL GHANY (*Ain Shams University, Faculty of Pharmacy, Analytical Chemistry Department, Cairo, Egypt, haitham.elfiky@fue.edu.eg)

      J. AOAC Int. 104, 19-25 (2022). HPTLC of xipamide (1) and triamterene (2) on silica gel with toluene - methanol - ethyl chloride - acetic acid 35:10:5:1. Quantitative determination by absorbance measurement at 300 nm. The hRF values for (1) and (2) were 52 and 37, respectively. Linearity was between 0.3 and 7.0 µg/zone for (1) and 0.3 and 12.0 µg/zone for (2). Inter-day and intra-day precisions were below 2 % (n=3). The LOD and LOQ were 47 and 141 ng/zone for (1) and 75 and 228 ng/zone for (2), respectively. Mean recovery was 100.2 % for (1) and 100.8 % for (2).

      Classification: 32a
      130 101
      Dual-mode gradient HPLC and TLC densitometry methods for the simultaneous determination of paracetamol and methionine in the presence of paracetamol impurities
      H. IBRAHIM*, A.M. HAMDY, H.A. MEREY, A.S. SAAD (*Anal. Chem. Dep., , Fac. of Pharmacy, Egyptian Russian Univ., Cairo-Suez Road, Badr City 11829, Egypt, hany.ibrahim@eru.edu.eg)

      J. AOAC Int. 104, 975-982 (2021). HPTLC of methionine (1) and paracetamol (2) on silica gel with butanol - dioxane - toluene - methanol 80:25:35:3. Quantitative determination by absorbance measurement at 220 nm. The hRF values for (1) and (2) were 26 and 83, respectively. Linearity was between 2 and 12 µg/zone for (1) 5 and 30 µg/zone for (2). Inter-day and intra-day precisions were below 1 % (n=3). The LOD and LOQ were 612 and 1856 ng/zone for (1) and 1525 and 4620 ng/zone for (2), respectively. Mean recovery was 101.3 % for (1) and 100.6 % for (2). 

      Classification: 32a
      130 029
      Efficient isolation of mycosporine-like amino acids from marine red algae by fast centrifugal partition chromatography
      M. ZWERGER, S. SCHWAIGER, M. GANZERA* (*Department of Pharmacognosy, Institute of Pharmacy, University of Innsbruck, Innsbruck, Austria; markus.ganzera@uibk.ac.at)

      Marine Drugs 20(2), 106 (2022). TLC was used to monitor the fractionation of hydro-methanolic extracts of Rhodophytes: Gracilaria gracilis (Gracilariaceae) (A), Porphyra sp. (Bangiaceae) (B), Spongoclonium pastorale (Ceramiaceae / Wrangeliaceae) (C); and to assess the purity of two isolated mycosporine-like amino acids: porphyra-334 and shinorine.  TLC on silica gel with n-butanol - acetic acid - water 3:1:1. Derivatization by spraying ninhydrin reagent for the detection of peptides and amino-acids; or by spraying anisaldehyde - sulfuric acid for most phytochemicals; in both cases, followed by 5 min heating at 100 °C. Visualization under white light and at 366 nm. Porphyra-334 (hRF 28) was isolated pure from (B) and (C). Shinorine (hRF 25), isolated from (A) and (B), contained a coeluting sugar (hRF 48), which was absent after further purification on solid phase extraction, and, only when isolated from (B), a coeluting peptide or amino-acid (hRF 35), which was absent after further purification on cyclodextrane column chromatography.

      Classification: 4d, 18a, 32e
      130 103
      Separation and determination of diflunisal and its impurity by two chromatographic methods: TLC–densitometry and HPLC
      N. FARID*, I. NAGUIB, R. MOATAMED, M. EL GHOBASHY (*Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmad Hegazy St, Beni-Suef, 62514, Egypt, radwasaeed84@gmail.com)

      J. AOAC Int. 104, 1719-1725 (2021). HPTLC of diflunisal (1) and its impurity biphenyl-4-ol (2) on silica gel with toluene - acetone - acetic acid 7:13:2. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 59 and 79, respectively. Linearity was between 0.5 and 3 µg/zone for (1) and 0.3 and 1.7 µg/zone for (2). Inter-day and intra-day precisions were below 1 % (n=3). The LOD and LOQ were 131 and 398 ng/zone for (1) and 72 and 219 ng/zone for (2), respectively. Mean recovery was 100.2 % for (1) and 100.5 % for (2). 

      Classification: 32a
      130 104
      DoE and Risk-Based DMAIC Principle for Implementation of Enhanced Analytical Quality by Design Approach to Multipurpose-Chromatography Method for Simultaneous Estimation of Multiple Fixed-Dose Combination Products of Aspirin
      P. PRAJAPATI*, K. JAYSWAL, S. SHAH (*Uka Tarsadia University, Maliba Pharmacy College, Department of Quality Assurance, Bardoli-Mahuva Road, Tarsadi, Mahuva, Surat-394 350, Gujarat, India, pintu21083@gmail.com)

      J. AOAC Int. 104, 1430-1441 (2021). HPTLC of eight different fixed-dose combination products of aspirin (1) with ramipril (2), caffeine (3), metoprolol (4), paracetamol (5), atorvastatin (6) and simvastatin (7) on silica gel with toluene - ethyl acetate - 1 % formic acid in methanol 35:65:3. Quantitative determination by absorbance measurement at 229 nm. The hRF values for (1) to (7) were 88, 15, 34, 49, 59, 66 and 78, respectively. Linearity was between 2000 and 6000 ng/zone for (1) and (5), 100 and 300 ng/zone for (2), 600 and 1800 ng/zone for (3), 1000 and 3000 ng/zone for (4), 200 and 600 ng/zone for (6) and 800 and 2400 ng/zone for (7). Inter-day and intra-day precisions were below 2 % (n=3). The LOD and LOQ were 107 and 323 ng/zone for (1), 32 and 98 ng/zone for (2), 17 and 51 ng/zone for (3), 3 and 10 ng/zone for (4), 9 and 27 ng/zone for (5), 109 and 331 ng/zone for (7), respectively. Recovery ranged 99.2-100.8 % for (1), 99.2-100.2 % for (2), 98.2-101.3 % for (3), 99.4-100.6 % for (4), 100.2-101.4 % for (5), 98.7-101.3 % for (6) and 99.6-101.3 % for (7).

      Classification: 32a
      130 106
      Characterization of quality differences of Ophiopogonis Radix from different origins by TLC, HPLC, UHPLC-MS and multivariate statistical analyses
      L. JIANG (Jian Ling), Y. QIU (Qiu Yixing), Z. CHEN (Chen Zhuliang), L. LUO (Luo Lu), H. TANG (Tang Hongxia), X. ZHOU (Zhou Xudong), H.YUAN (Yuan Hangwen), W. WANG (Wang Wei)*, P. LIU (Liu Pingan) (*TCM and Ethnomedicine Innovation and Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China, wangwei402@hotmail.com)

      J. Liq. Chromatogr. Relat. Technol. https://doi.org/10.1080/10826076.2022.2159977 (2021). HPTLC of Ophiopogonis Radix from different habitats on silica gel with toluene - methanol - glacial acetic acid 800:50:1. Detection under UV light at 254 nm. 

       

      Classification: 32e
      130 028
      The effect of extractive lacto-fermentation on the bioactivity and natural products content of Pittosporum angustifolium (gumbi gumbi) extracts
      Snezana AGATONOVIC-KUSTRIN*, V. GEGECHKORI, D.W. MORTON
      (*Department of Pharmaceutical and Toxicological Chemistry, Institute of Pharmacy, Sechenov University, Moscow, Russia, and School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Bendigo, Australia; s.kustrin@latrobe.edu.au)

      J Chromatogr A, 1647, 462153 (2021). Samples were extracts of Pittosporum angustifolium leaves (Pittosporaceae), either pure or fermented 1-4 weeks in NaCl solution, as well as acarbose, gallic acid, β-sitosterol, caffeic and chlorogenic acids, as standards. HPTLC on silica gel (prewashed with methanol and dried 15 min at 105 °C) with n-hexane – ethyl acetate – acetic acid 15:9:1. Derivatization by immersion (speed 5 cm/s, time 1 s): (A) into DPPH• 0.2 % solution, to detect radical scavengers; (B) into neutralized ferric chloride (3 % in ethanol), followed by 5 min heating at 110 °C, for detection of phenolic compounds; (C) into anisaldehyde – sulphuric acid reagent, followed by 10 min heating at 110 °C, to detect terpenes and steroids. Effect-directed analysis (EDA) for α-amylase inhibition assay (D) by immersion into enzyme solution, incubation 15 min at 37 °C, immersion into substrate solution (starch 2 % in water), incubation 20 min at 37 °C and immersion into Gram’s iodine solution for detection (inhibition zones appear blue on white background). In all cases, visualization under white light. Quantification was performed on pictures using image processing software, and expressed as equivalents to the respective standards used for calibration curves: (A) and (B) gallic acid (LOQ 250 and 740 ng/band, respectively), (C) β-sitosterol (LOQ 1.5 µg/band), (D) acarbose (LOQ 8 µg/band). Zones of interest, scraped from untreated plates and washed with ethyl acetate, were submitted by ATR-FTIR analysis. An amylase inhibiting zone (hRF 85) present in all extracts was identified as fatty acid esters: ethyl palmitate in unfermented and methyl linoleate in fermented extracts. Moreover, fermented extracts contained antioxidant zones (hRF 15 – 20), identified as monomers and oligomers (including hydroxycinnamic, guaiacyl, syringyl derivatives) from decomposed lignin.

      Classification: 4e, 7, 8b, 11a, 32e
      130 013
      Characterization of natural herbal medicines by thin-layer chromatography combined with laser ablation-assisted direct analysis in real-time mass spectrometry
      Y. CHEN (Chen Yilin), L. LI (Li Linnan)*, R. XU (Xu Rui), F. LI (Li Fan), L. GU (Gu Lihua), H. LIU (Liu Huwei), Z. WANG (Wang Zhengtao), L. YANG (Yang Li)** (*Shanghai Key Laboratory of Compound Chinese Medicines, and Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China; **Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China; *linnanli@shutcm.edu.cn, **yl7@shutcm.edu.cn)

      J Chromatogr A, 1625, 461230 (2020). Samples were extracts of Chinese plants: Acorus tatarinowii (= Acorus calamus var. angustatus) rhizomes (Araceae / Acoraceae) (1), Angelica sinensis roots (Apiaceae) (2), Gynura japonica rhizomes (Asteraceae) (3), Phellodendron chinense bark (Rutaceae) (4), Picrasma quassioides twigs and leaves (Simaroubaceae) (5), Rheum sp. roots and rhizomes (R. palmatum, R. tanguticum and/or R. officinale) (Polygonaceae) (6), Sophora flavescens roots (Fabaceae) (7), Dendrobium stems (D. aphyllum, D. aurantiacum var. dennaeanum, D. chrysanthum, D. chrysotoxum, D. gratiosissimum, D. hercoglossum, D. thyrsiflorum, D. trigonopus and D. williamsonii) (Orchidaceae) (8). Standards were: gigantol (from D. sonia); methoxycarbonyl-β-carboline (MCC from (5)); caffeic acid, emodin; senecionine and β-asarone; crategolic acid (= maslinic acid), corosolic acid, oleanic acid, ursolic acid; sesquiterpenoids (atractylenolides I – III) from Atractylodes macrocephala (Asteraceae); flavonoids (baicalein, baicalin, daidzin, hesperidin, wogonin) from Scutellaria baicalensis roots (Lamiaceae). HPTLC on silica gel with 10 mobile phases, depending on the samples. Detection under UV 254 nm and white light. For (3), derivatization with Dragendorff’s reagent (bismuth potassium iodide solution) for visualization of alkaloids. Zones of interest on underivatized plates were identified by a triple-quadrupole ­– linear ion-trap MS, the compounds being removed from the layer by a continuous-wave (445 nm) diode laser pointer through a DART interface (Direct Analysis in Real-Time, helium as gas for plasma-based ambient ionization, discharge needle voltage 1.5 kV, grid voltage 350 V, capillary temperature 300 °C and voltage 40 V, full scan in positive ionization mode in m/z range 150-800). Pigment standards were used for validation of this laser-assisted HPTLC-DART-MS method: malachite green, crystal violet, chrysoidin, auramine O, rhodamine B, Sudan red I – IV, Sudan red G, dimethyl yellow. Afterwards, the same HPTLC-MS method was applied to the origin / species determination of Dendrobium samples, based on the presence of four bibenzyl compounds erianin, gigantol, moscatilin, tristin. Erianin was present only in D. chrysotoxum, whereas none of these were detected in D. hercoglossum. Several components of the extracts were thus identified: asarone (a phenylpropanoid) in (1); phthalide lactones (butenylphthalide, ligustilide and chuanxiong lactone) in (2); co-eluting pyrrolizidine alkaloids (senecionine and seneciphylline) in (3); benzylisoquinoline alkaloid berberine in (4); alkaloids (canthinone alkaloids and MCC) in (5); anthraquinones (rhein, aloe-emodin, emodin, emodin methyl ether, chrysophanol) and (in negative mode) caffeic acid (a hydroxycinnamic acid) and corosolic, maslinic and oleanic acids (triterpenoids) in (6); quinolizidine alkaloids (matrine, oxymatrine, oxysophocarpine, sophoridine) in (7).

      Classification: 4e, 7, 8a, 8b, 15a, 22, 32e
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